| Project/Area Number |
13660172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
LEE Tai-hung Hokkaido Univ., Grad. Of Fisheries Science, Instructor, 大学院・水産科学研究科, 助手 (60210811)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Tsukasa Nihon Univ. College of Bioresource Sciences, Lecturer, 生物資源科学部, 講師 (60241379)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | androgenic gland / androgenic gland hormone / crustacean / Macrobrachium rosenbergii / decapod / prawn / sex differentiation |
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| Research Abstract |
Based on the genetic information of Armadillidium vulgare's androgenic gland hormone (AGH), this study aimed to find a way of screening AGH gene in the prawn Macrobarachium rosenbergii, and to study the mechanism of its expression. In addition, we made use of the phenomenon that androgenic glands hypertrophy after the eye stalks of the animals are ablated and developed a reliable method for obtaining and androgenic glands whose AGH gene is in expression.
On the 3rd, 7th and 15th days following the of the ablation of eye stalks, androgenic glands were removed and used for RNA analysis. Northern hybridization was performed using the RNA from A. vulgare but there were no signals in any samples, indicating that the amount of the AGH gene expression in the tissues was extremely small even after the ablation of the eye stalks. Therefore, to increase AGH RNA, SP6 or T7 primmer sites were fused to oligo-dt primmer, and used for synthesis of cDNA libraries. Then, PCR and 3'-Race were performed using the libraries after RNA synthesis using RNA polymerase and 360bp of a gene fragment was obtained. The gene showed 84.16% of similarity to the AGH of A. vulgare.
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