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MECHANISMS IN EXPRESSIONS OF TWO FORMS OF VITELLOGENINS IN FISH

Research Project

Project/Area Number 13660175
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

ADACHI Shinji  Hokkaido Univ., Grad. School of Fish. Sci., Asso. Pro., 大学院・水産科学研究科, 助教授 (40231930)

Co-Investigator(Kenkyū-buntansha) MATSUBARA Takahiro  Hokkaido Natl. Fish. Res. Inst., Head Researcher, 北海道区水産研究所, 室長
HARA Akihiko  Hokkaido Univ., Grad. School of Fish. Sci., Pro., 大学院・水産科学研究科, 教授 (40091483)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsVitellogenin / Japanese goby / Gene expression / Phosvitin / Lipovitellin / β'-Component / Estradiol-17β / Teleost / エストラジールー17β
Research Abstract

Recently, two forms of vitellogenin (Vg) were found in Japanese goby. In this study, we investigated the hormonal regulations of the two forms of Vg using liver culture system.
Organ culture with slices from both male and female liver was attempted for the analyses. The Vg levels were measured inculture media by newly developed specific enzyme-linkedimmunosorbent assay for the each Vg (530kVg and 320kVg) after the incubation for 7 days with various doses of estradiol-17β (E2).
In male liver, almost the same amount of 530k and 320kVgwere produced in response to E2 in dose dependent manner. Incontrast, the levels of both Vgs in female liver at vitellogenic stage were apparently higher than those in male liver, although 2-3fold 530kVg was detected as compared with 320kVg. Inaddition, the levels of both Vgs produced by the female liver incubated without E2 were not significantly different from those with E2.
These results suggest that 530kVg and 320kVg were regulated differentially.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Ohashi: "Production of two forms of vitellogenin in cultured liver of Japanese goby (Acanthogobius flavinonus)"Fisheries Sci.. 68. 969-970 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsubara: "Changes in mRNA levels of vitellogenin 1 and 2 during artificial maturation in Japanese eel, Anguilla japonica"Fisheries Sci.. 68. 971-972 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohkubo: "Development of enzyme-linked immunosorbent assays (ELISAs) for two forms of vitellogenin in Japanese Common Goby (Acanthogobius flavimanus)"Gen. Comp. Endocrinol.. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohashi et al.: "Production of two forms of vitellogenin in cultured liver of Japanese goby (Acanthogobius flavinonus)"Fisheries Sci.. 68. 969-970 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsubara et al.: "Changes in mRNA levels of vitellogenin 1 and 2 during artificial maturation in Japanese eel, Anguilla japonica"Fisheries Sci.. 68. 971-972 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohkubo et al.: "Development of enzyme-linked immunosorbent assays (ELISAs) for two forms of vitellogenin in Japanese Common Goby (Acanthogobius flavimanus)"Gen. Comp. Endocrinol.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ohashi: "Production of two forms of vitellogenin in cultured liver of Japanese goby (Acanthogobius flavinonus)"Fisheries Sci.. 68. 969-970 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsubara: "Changes in mRNA levels of vitellogenin 1 and 2 during artificial maturation in Japanese eel, Anguilla japonica"Fisheries Sci.. 68. 971-972 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohkubo: "Development of enzyme-linked immunosorbent assays (ELISAs) for two forms of vitellogenin in Japanese Common Goby (Acanthogobius flavimanus)"Gen. Comp. Endocrinol.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohashi: "Production of two forms of vitellogenin in cultured liver of Japanese goby (Acanthogobius flavinonus)"Proceedings of the 70th anniversary international commemorative symposium of JSFS. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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