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Studies on the production of Porphyrae yezoensis reeds tolerant to the red rot disease by the gene transduction of anti-Pythium protein

Research Project

Project/Area Number 13660186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionSaga University

Principal Investigator

KAMEI Yuto  Saga University, Costal Bioenvironment Center, Associate Professor, 海浜台地生物環境研究センター, 助教授 (50192547)

Project Period (FY) 2001 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsthe Ariake Sea / Porphyrae yezoensis / red rot disease / Pythium porphyrae / disease-tolerant Porphyrae yezoensis / antifungal protein / cell wall degrading activity / gene transduction / 真菌ピシウム / ノリプロトプラスト / ノリ細胞壁分解酵素 / 組換え体粗酵素 / 組換え作物 / 抗ピシウムタンパク / 作用機序 / 膜透過性 / ピシウム菌 / Pseudomonas sp.PE2 / キチナーゼA / 糖質結合領域 / polycystic kidney diseasse / 抗真菌性遺伝子 / 耐病性 / プロトプラスト
Research Abstract

In the present study, we conducted to screen the antifungal proteins against Pythium porphyare to establish the new breeds of Pophyrae yezoensis tolerant to the red rot disease, and isolated 13 strains of bacteria with the cell wall degrading activity to P. porphyare from the Ariake Sea. Pseudomoas sp. PE2, the most potent active bacterium showed chitin, β-1,3;1,4-glucan, and β-1,3-glucan degrading activities and then these genes were cloned by the expression of these enzymes. When we analyzed these genes, these proteins have been proved to have the functional regions with a variety of binding activities to several polysaccharides and specifically bound to some polysaccharides. Among these enzymes, β-1,3(4)-glucanase A has been found to be most important for the cell wall degradation. More over, when we purified and characterized the anti-P. porphyare protein (SAP) from the culture supernatant of the marine bacterial isolate, Sreptomyces sp. AP77, SAP was proved to have three subunits and the homologies of these subunits are very high to the known proteins. The mode of action of SAP seems to be related to the permeability of cell membrane of P.porhyrae.
Thus, the present study proves that above enzymes and antifungal proteins are very useful and expectable to establish the disease-tolerant breeds of P.yezoensis by the gene transduction of those proteins.

Report

(5 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All 2005 2003 2002 Other

All Journal Article (10 results) Publications (6 results)

  • [Journal Article] ノリプロトプラスト化能を有する海洋細菌Pseudomonas sp. ND137株由来のβ-1,4キシラナーゼ遺伝子のクローニング2005

    • Author(s)
      松本雅子, 青木幸久, 亀井勇統
    • Journal Title

      Coastal Bioenvironment 4

      Pages: 45-52

    • NAID

      110001077555

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Molecular cloning, sequencing and expression of the gene encoding a novel chitinase A from a marine bacterium, Pseudomonas sp. PE2 and its domain structure2003

    • Author(s)
      E.Kitamura, Y.Kamei
    • Journal Title

      Applied Microbiology and Biotechnology 61

      Pages: 140-149

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Antifungal mechanism of an anti-Pythium protein (SAP) from the marine bacterium, Streptomyces sp. AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp.2003

    • Author(s)
      J.H.Woo, Y.Kamei
    • Journal Title

      Applied Microbiology and Biotechnology 62

      Pages: 407-413

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning, sequencing and expression of the gene encoding a novel chitinase A from a marine bacterium, Pseudomonas sp. PE2 and its domain structure.2003

    • Author(s)
      E.Kitamura, Y.Kamei
    • Journal Title

      Applied Microbiology and Biotechnology 61

      Pages: 140-149

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Molecular cloning, sequencing and expression of the gene encoding a novel chitinase A from a marine bacterium, Pseudomonas sp. PE2, and its domain structure2003

    • Author(s)
      Etsushi Kitamura, Yuto Kamei
    • Journal Title

      Applied Microbiology & Biotechnology 61

      Pages: 140-149

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Antifungal mechanism of an anti-Pythium protein (SAP) from the marine bacterium, Streptomyces sp. AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp..2003

    • Author(s)
      Jung-Hee Woo, Yuto Kamei
    • Journal Title

      Applied Microbiology & Biotechnology 62

      Pages: 407-413

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Polysaccharolytic activities of bacterial enzymes that degrade the cell walls of Pythium porphyrae, a causative fungus of red rot disease in Porphyra yezoensis2002

    • Author(s)
      E.Kitamura, H.Myouga, Y.Kamei
    • Journal Title

      Fisheries Sciences 68

      Pages: 436-445

    • NAID

      10008273486

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] An antifungal protein from the marine bacterium, Streptomyces sp. AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp2002

    • Author(s)
      J.H.Woo, E.Kitamura, E.Myouga, Y.Kamei
    • Journal Title

      Applied and Environmental Microbiology 68

      Pages: 2666-2675

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Polysaccharolytic activities of bacterial enzymes that degrade the cell walls of Pythium porphyrae, a causative fungus of red rot disease in Porphyra yezoensis.2002

    • Author(s)
      E.Kitamura, H.Myouga, Y.Kamei
    • Journal Title

      Fisheries Sciences 68

      Pages: 436-445

    • NAID

      10008273486

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] protein from the marine bacterium, Streptomyces sp. AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp.2002

    • Author(s)
      J.H.Woo, E.Kitamura, E.Myouga, Y.Kamei
    • Journal Title

      Applied and Environmental Microbiology 68

      Pages: 2666-1675

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Etsushi Kitamura: "Molecular cloning, sequencing and expression of the gene encoding a novel chitinase A from a marine bacterium, Pseudomonas sp. PE2 and its domain structure"Applied Microbiology & Biotechnology. 61. 140-149 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Jung-Hee Woo: "Antifungal mechanism of an anti-Pythium protein (SAP) from the marine bacterium, Streptomyces sp.AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp."Applied Microbiology & Biotechnology. 62. 407-413 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Jung-Hee Woo, Etsushi Kitamura, Hisashi Myoga, Yuto Kamei: "An Antifungal Protein from the Marine Bacterium, Streptomyces sp.AP77 is Specific to Pythium porphyrae, a Causative Agnet of Red Rot Disease in Porhyra"Applied and Environmental Microbiology. 68・6. 2666-2675 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Etsushi Kitamura, Yuto Kamei: "Molecular cloning, sequencing and expression of the gene encoding a novel chitinase A from a marine bacterium, Pseudomonas sp. SE2 and its domain structure"Applied Microbiology & Biotechnology. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Etsushi Kitamura, Hisashi Myouga, Yuto Kamei: "Polysaccharolytic Activities of Bacterial Enzymes That Degrade the Cell Walls of Pythium porphyrae, a Causative Fungus of Red Rot Disease in Porphyra yezoensis"Fisheries Sciences. 68・2. 436-445 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Jung-Hee Woo, Etsushi Kitamura, Hisashi Myoga, Yuto Kamei: "An Antifungal Protein from the Marine Bacterium, Streptomyces sp. AP77 is Specific to Pythium porphyrae, a Causative Agent of Red Rot Disease in Porphyra spp"Applied and Environmental Microbiology. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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