• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Basic study on the development of "Bio-Defence Foods" by DNA motifs from dairy lactic acid bacteria

Research Project

Project/Area Number 13660265
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionTohoku University

Principal Investigator

KITAZAWA Haruki  Tohoku University, Agriculture, Associate Professor, 大学院・農学研究科, 助教授 (10204885)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsProbiotics / L.bulgaricus / L.gasseri / DNA / cytokine / Peyer's patch / TH1 / Lactic acid bacteria / L.bulgaricu / 樹状細胞 / 走化性因子
Research Abstract

Fermented milks and their related dairy lactic acid bacterla have long been thought to be of value for health maintenance. Recently, corroborative experiments on the physiological functions of dairy lactic acid bacteria (LAB), especially the probiotic LAB, and their derivatives have been carried out. A great interest has been focused on biological functions such as immunomodulations. In our recent study, we have been particularly focusing on the DNA from LAB as one of the immunomoduratory factors in the LAB. The present study was conducted to elucidate the cytokine induction by the DNA in the gut. After stimulation of swine peyer's patch and mesenteric lymph node wlth DNA motifs from the LAB, induction of cytokine mRNA was estimated by real time PCR method. In addition, a novel function of L gasseri was investigated. The DNA motifs augmented induction of TH1 type cytokine (IL-6, IL-12, IFNy) mRNA in peyer's patches. The macrophage clemotaxis induced by L gasseri JCM1131^T culture supematants, which the chemotactic factor was designated as "Gasserokine", was discovered to be a new biological function exerted by probiotic lactic acid bacteria. Therefore, the activity is expected to be used for one of the functional parameters in the immunomodulating properties of probiotic lactic acid bacteria. This study demonstrated that immunostimulatory DNA motif from the LAB exerted the cytokine induction in the gut and proposed that the LAB is a good candidate of a starter culture for the production of new functional foods, "Bio-Defense Foods".

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Kitazawa H.et al.: "A novel immunostimulating aspect of Lactobacillus gasseri : induction of "Gasserokine" as chemoattractants for macrophages"Int.J.Food Microbiol.. 77. 29-38 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kitazawa H.et al.: "Rapid activation of Mac-1(CD11b/CD18) molecules on macrophages by a new chemotactic factor 'Gasserokine' produced by Lactobacillus gasseri JCM1131^T."Animal Sci.J.. 73. 395-401 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kitazawa H.et al.: "Discovery of immunostimulatory DNA motifs from lactic acid bacteria and their application to "Bio-Defence Foods""Biosci.Biotechnol.Biochem.. 76. 833-836 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kitazawa H.et al.: "Immunostimulatory oligonucleotide, CpG like motif exists in Lactobacillus delbrueckii ssp. bulgaricus NIAIB6"Int.J.Food Microbiol.. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimosato, T., H.Kitazawa et al.: "Swine Toll-like receptor 9 recognizes CpG motifs of human cell stimulant"Biochim.Biophys.Acta. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Haruki Kitazawa: "A novel immunostimulating aspect of Lactobacillus gasseri induction of "Gasserokine" as chemoattractants for macrophages"Int.J.Food Microbiol.. 77. 29-38 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Haruki Kitazawa: "Rapid activation of Mac-1(CD11lb/CD18) molecules on macrophages by a new chemotactic factor 'Gasserokine' produced by Lactobacillus gasseri JCM1131^T"Animal Sci.J.. 73. 395-401 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Haruki Kitazawa: "Dlscovery of immunostimulatory DNA motifs from lactic acid bacteria and their application to "Bio-Defence Foods""Biosci.Biotechnol.Biochem.. 76. 833-836 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Haruki Kitazawa: "Immunostimulatory oligonucleotide, CpG like motif exists in Lactobacillus delbrueckii ssp. bulgaricus NIAIB6"Int.J.Food Microbiol.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takeshi Shimosato: "Swine Toll-like receptor 9 recognizes CpG motifs of human cell stimulant"Biochim.Biophys.Acta.. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kitazawa, H. et al.: "A novel immunostimulating aspect of Lactobacillus gasseri : induction of "Gasserokine" as chemoattractants for macrophages"Int. J. Food Microbiol.. 77. 29-38 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kitazawa, H. et al.: "Rapid activation of Mac-1(CDI11b/CD18) molecules on macrophages by a new chemotactic factor 'Gasserokine' produced by Lactobacillus gasseri JCM1131^T"Animal Sci. J.. 73. 395-401 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kitazawa, H. et al.: "Discovery of immunostimulatory DNA motifs from lactic acid bacteria and the application to "Bio-Defence Foods""Biosci. Biotechnol. Biochem.. 76. 833-836 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kitazawa, H. et al.: "Immunostimulatory oligonucleotide, CpG like motif exists in Lactobacillus delbrueckii ssp. bulgaricus NIAIB6"Int. J. Food Microbiol.. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimosato, T., H.Kitazawa et al.: "Swine Toll-like receptor 9 recognizes CpG motifs of human cell stimulant"Biochim. Biophys. Acta. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kitazawa, H. et al.: "A novel immunostimulating aspect of Lactobacillus gasseri : induction of "Gasserokine" as chemoattractants for macrophages"Int. J. Food Microbiol.. (in press).

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi