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A cell-autonomous activity of mouse Sox17 gene in mouse embryogenesis

Research Project

Project/Area Number 13660295
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

KANAI Yoshiakira  The University of Tokyo, Graduate School of Agriculture and Life Sciences, Research Associate, 大学院・農学生命科学研究科, 助手 (30260326)

Co-Investigator(Kenkyū-buntansha) HAYASHI Yoshihiro  Graduate School of Agriculture and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (90092303)
YONEKAWA Hiromichi  The Tokyo Metropolitan Institute of Medical Science, Vice-director, 実験動物研究部, 副所長(研究職) (30142110)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsSox17 / Endoderm / ES cells / Gut / ノックアウトマウス
Research Abstract

In the mouse, the definitive endoderm is derived the epiblast during gastrulation, and, at the early organogenesis stage, forms the primitive gut tube, which gives rise to the digestive tract, liver, pancreas and associated visceral organs. The transcriptional factors, Sox17 (a Sry-related HMG box factor) and its upstream factors, Mixer and Casanova, have been shown to function as endoderm determinants in Xenopus and zebrafish, respectively. However, whether the mammlian orthologues of these genes are also involved with endoderm formation is not known. We show that Sox17^<-/-> mutant embryos are deficient of gut endoderm. The earliest recognisable defect is the reduced occupancy by the definitive endoderm in the posterior and lateral region of the prospective mid-and hundgut of the headfold stage embryo. The prospective forgut develops properly until the late neural place stage. Thereafter, elevated levels of apoptosis lead to a reductin in the population of the definitive endoderm in the forgut. In addition, the mid- and hingut tissues fail to expand. These are accompanied by the replacement of the definitive endoderm in the lateral region of the entire length of the embryonic gut by cells that resemble the visceral endoderm. In the chimeras, although Sox17-null ES cells can contribute unrestrictedly to ectoderm and mesodermal tissues, few of them could colonize the forgut endoderm and they are completely excluded from the mid- and hindgut endoderm. Our findings indicate an important role of Sox17 in endoderm development in the mouse, highlighting the idea that the molecular mechanisms for endoderm formation in likely to be conserved among vertebrates.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Kanai-Azuma M, Kanai Y, 他9名: "Depletion of definitive gut endoderm in Sox17-null mutant mice"Development. 129(10). 2367-2379 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Noma T, Kanai Y, 他7名: "Stage-and sex-dependent expressions of Usp9x, an X-linked mouse orthologue of Drosophila Fat facets, during gonadal development and oogenesis in mice"Cenc Expr. Patterns (the section of Mech. Dev.). 2. 87-91 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 水上拓郎, 金井克晃 他5名: "マウス末分化生殖原基の器官培養による精巣、卵巣への分化誘導"J. Reprod. Develop.. 48. XX-XXIII (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 金井克晃 他7名: "哺乳類の生殖腺の性分化-SryからMis/テストステロン分泌まで"比較内分泌学会ニュース. 106. 14-21 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakano, K, Kanai-Azuma, M, Kanai Y, 他4名: "Cofilin Phosphorylation and Actin Polymerization by NRK/NESK, a Member of the Germinal Center Kinase Family"Exp. Cell Res.. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tam PPL, Kanai-Azuma, M, Kanai Y.: "Early endoderm development in vertebrates : lineage differentiation and morphogenetic function"Curr. Opin. Genet. Dev.. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tam, P.P.L., Masami Kanai-Azuma, and Yoshiakira Kanai: "Early endoderm development in vertebrates : lineage differentiation and morphogenetic function"Curr Opin Genet Dev. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakano K, Kanai-Azuma M, Kanai Y, Moriyama K, Yazaki K, Hayashi Y, and Kitamura N: "Cofilin Phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family"Exp Cell Res. in press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kanai-Azuma M., Kanai Y., Gad J.M., Tajima Y., Taya C., Kurohmaru M., Sanai Y., Yonekawa H., Yazaki K., Tam P.P.L, and Hayashi Y.: "Depletion of definitive gut endoderm in Soxl7-null mutant mice"Development. 129(10). 2367-2379 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Noma T., Kanai Y., Kanai-Azuma M., Ishii M., Fujisawa M., Kurohmaru M., Kawakami H., Wood SA., and Hayashi Y.: "Stage- and sex-dependent expressions of Usp9x, and X-linked mouse orthologue of Drosophila Fat facets, during gonadal development and oogenesis in mice"Gene Expr Patterns (Mech. Dev.). 2. 87-91 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kanai-Azuma M, Kanai Y, 他9名: "Depletion of definitive gut endoderm in Sox17-null mutant mice"Development. 129(10). 2367-2379 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Noma T, Kanai Y, 他7名: "Stage-and sex-dependent expressions of Usp9x, an X-linked mouse orthologue of Drosophila Fat facets, during gonadal development and oogenesis in mice"Cene Exp.Pattern (the section of Mech.Dev.). 2. 87-91 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 水上拓郎, 金井克晃他5名: "マウス末分化生殖原基の器官培養による精巣、卵巣への分化誘導"J.Reprod.Develop.. 48. XX-XXIII (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 金井克晃, 他7名: "哺乳類の生殖腺の性分化-SryからMis/テストステロン分泌まで"比較内分泌学会ニュース. 106. 14-21 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakano, K, Kanai-Azuma, M, Kanai Y, 他4名: "Cofilin Phosphorylation and Actin Polymerization by NRK/NESK, a Member of the Germinal Center Kinase Family"Exp.Cell Res.. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tam PPL, Kanai-Azuma, M, Kanai Y.: "Early endoderm development in vertebrates : lineage differentiation and morphogenetic function"Curr.Opin.Genet.Dev.. (印刷中). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kanai-Azuma, et al.: "Depletion of definitive gut endoderm in Sox17-null mutant mice"Development. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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