| Project/Area Number |
13660297
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Gifu University |
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Principal Investigator |
TAKEWAKI Tadashi Gifu University, Agriculture, Professor, 大学院・連合獣医学研究科, 教授 (00021717)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Yasutake Gifu University, Agriculture, Associate Professor, 農学部, 助教授 (40243802)
UNNO Toshihiro Gifu University, Agriculture, Associate Professor, 農学部, 助教授 (90252121)
KOMORI Seiichi Gifu University, Agriculture, Professor, 農学部, 教授 (70195866)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | vascular tone / blood flow / sympathelic / endothelium / interplay / hibernation / small artery / rasular resistance / 末消血流 / 神経 |
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| Research Abstract |
The interaction between sympathetic nerves and endothelial cell was investigated by measuring the changes in smooth muscle membrane potential or muscle tone. The present experiments provide evidence that ATP released from sympathetic nerve terminals innervating hamster mesenteric arteries induces release of endothelium-derived hyperpolarizing factor (EDHF) from endothelial cells. Neurally released ATP evoked biphasic responses that were initially excitatory junction potentials (a direct action) on the smooth muscle. This is the first demonstration of neurally released ATP mediating hyperpolarization through EDHF. The present findings demonstrate that ATP released from the sympathetic nerves may diffuse as far as the endothelium and activate P2Y_2-like receptors to induce the release of EDHF in thin arteries, but not in thick arteries. EDHF appears to be a primary functional factor in small arteries, thus it's proposed that EDHF released by neural ATP may play an important physiological role in the local regulation of vascular resistance. Additionally, we established that UTP released EDHF, which hyperpolarized the smooth muscle cells of hamster mesenteric artery. EDHF-mediated purinergic neuromodulation may play a very important physiological role in the control of vascular resistance in small arteries.
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