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Pharmacological characterization of prostanoid receptors present in the porcine uterus

Research Project

Project/Area Number 13660304
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionRakuno Gakuen University

Principal Investigator

TANEIKE Tetsuro  Rakuno Gakuen University, School of Veterinary Medicine, Professor, 獣医学部, 教授 (30048110)

Co-Investigator(Kenkyū-buntansha) KITAZAWA Takio  Rakuno Gakuen University, School of Veterinary Medicine, Associate Professor, 獣医学部, 助教授 (50146338)
YOKOTA Hiroshi  Rakuno Gakuen University, School of Veterinary Medicine, Professor, 獣医学部, 教授 (90137414)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsPorcine uterus / Prostaglandins / Prostanoid EP receptor / Prostanoid DP receptor / Prostanoid FP receptor / Prostanoid TP receptor / Pregnancy / cyclooxygenase / Prostanoid FP受容体 / Prostanoid DP受容体 / Prostanoid EP受容体 / 子宮角 / 子宮頸管 / Prostanoid 受容体 / 子宮筋(ブタ) / Thromboxane / TP 受容体 / 筋層差 / 子宮収縮薬 / 子宮収縮制御 / Prostanoid受容体
Research Abstract

In the present research project, prostanoid receptor subtype and distribution of cyclooxygenase (COX) in the porcine uterus were characterized using pharmacological and biochemical techniques, and physiological roles of prostanoid in regulation of myometrial contractility were discussed. Contractile (EP1, EP3, FP and TP) and relaxant prostanoid receptors (DP, IP and EP2) were demonstrated in the porcine uterus muscle and these receptor populations were consistent with human. Therefore the porcine uterus might be a useful animal model for assessing the mechanical actions of prostanoid receptor agonists in the human uterus. Prostanoid receptors in the porcine uterus was suggested to distribute heterogeneously depending on smooth muscle types (longitudinal and circular muscle) and uterine regions (cornu, corpus and cervix). In the longitudinal muscle, responsiveness to contractile prostanoid receptor agonists was high compared with the circular muscle and decreased gradually from cornu to … More cervix. In contrary, responsiveness to relaxant prostanoid receptor agonists was low in the cornu and increased toward cervix. On the other hand, although responses to relaxant prostanoid receptor agonists were high in the circular muscle, regional difference in responsiveness to both contractile and relaxant prostanoid receptor agonists was not marked in comparison with the case of longitudinal muscle. The heterogeneous distribution of contractile and relaxant prostanoid receptor contributes to produce a gradient of the intra-luminal pressure from the cornu (high) to cervix (low) that is convenient for transportation of luminal contents. COX-1 but not COX-2 was expressed in the non-pregnant porcine uterus and distributed heterogeneously depending on muscle layer and uterine regions as did contractile prostanoid receptors. Inhibitors of COX and thromboxane A2 synthase decreased the spontaneous contraction of longitudinal muscle but not circular muscle. Present results suggest that prostanoid modulates, spontaneous myometrial contractility in pig. In the uterus of pregnant pig, COX-2 was expressed intensely and the responsiveness to a FP receptor agonist increased, suggesting a pregnancy-associated change in the physiological role of COX-prostanoid pathway. Further examinations might be necessary to clarify how steroid hormones regulate prostanoid receptor and COX expression, and myometrial motility. Less

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Shayibuzhati M, Hatakeyama H, Tajima T, Kitazawa T, Taneike T: "ブタ子宮筋に存在するプロスタノイド受容体サブタイプの薬理学的解析"J Smooth Muscle Res.. 5. J69-J80 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cao J, Shayibuzhati M, Tajima T, Kitazawa, T, Taneike T: "In vitro pharmacological characterization of the prostanoid receptor polulation in the non-pregnant porcine myometrium"European J Pharmacology. 442. 115-123 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inoue M, Kitazawa T, Cao J, Taneike T: "5-HT7 receptor-mediated relaxation of the oviduct in nonpregnant proestrous pigs"European J Pharmacology. 461. 207-218 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kitazawa T, Hatakeyama H, Cao J, Taneike T: "Pregnancy-associated changes in responsiveness of the porcine myometrium to bioactive substances"European J Pharmacology. 469. 135-144 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cao J, Wakatsuki A, Yoshida M, Kitazawa T, Taneike T: "Thromboxane A_2 (TP) receptor in the non-pregnant porcine myometrium and its role in regulation of spontaneous contractile activity"European J Pharmacology. 485. 317-327 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shayibuzhati M, Hatakeyama H, Tajima T, Kitazawa T, Taneike T: "Pharmacological characterization of prostanoid receptors in non-pregnant porcine myometrium"J Smooth Muscle Res.. 5(2). 169-180 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cao J, Shayibuzhati M, Tajima T, Kitazawa T, Taneike T: "In vitro pharmacological characterization of the prostanoid receptor population in the non-pregnant porcine myometrium"European J Pharmacology. 442. 115-123 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Inoue M, Kitazawa T, Cao I, Taneike T: "5-HT7 receptor mediated relaxation of the oviduct in nonpregnant proestrous pigs."European J Pharmacology. 461. 207-218 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kitazawa T, Hatakeyama T, Cao J, Taneike T: "Pregnancy-associated changes in responsiveness of the porcine myometrium to bioactive substances."European J Pharmacology. 469. 135-144 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cao J, Wakatsuki A, Yoshida M, Kitazawa T, Taneike T: "Thromboxane A2 (TP) receptor in the non-pregnant porcine myometrium and its role in regulation of spontaneous contractile activity."European J Pharmacology. 485. 317-327 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Cao J, Wakatsuki A, Yoshida M, Kitazawa T., Taneike T.: "Thromboxane A2 (TP) receptor in the non-pregnant porcine myometrium and its role in relation of spontaneous contractile activity."European J Pharmacology. 485(1-3). 317-327 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kitazawa T, Hatakeyama H, Cao J, Taneike T.: "Pregnancy-associated changes in responsiveness of the porcine myometrium to bioactive substances."European J Pharmacology. 469(1-3). 135-144 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Cao, J., Shayibuzhati, M., Tajima, T., Kitazawa, T., Taneike, T.: "In vitro pharmacological characterization of the prostanoid receptor population in the non-pregnant porcine myometrium"European Journal of Pharmacology. 442. 115-123 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Inoue, M., Kitazawa, T., Cao, J., Taneike, T.: "5-HT7 receptor-mediated relaxation of the oviduct in non-pregnant proestrus pigs"European Journal of Pharmacology. 461. 207-218 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kitazawa, T., Hatakeyama, H., Cao, J., Taneike, T.: "Pregnancy-associated changes in responsiveness of the porcine myometrium to bioactive substances"European Journal of Pharmacology. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 沙衣布扎提米克熱木, 畠山洋文, 田島剛, 北澤多喜雄, 種池哲朗: "ブタ子宮筋に存在するプロスタノイド受容体サブタイプの薬理学的解析"J.Smooth Muscle Res (Jap Section). 5. J69-J80 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Cao J, Shayibuzhati M, Tajima T, Kitazawa T, Taneike T.: "In vitro pharmacological characterization of the prostanoid receptor population in the non-pregnant porcine myometrium"Eur.J.Pharmacol.. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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