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Pathogenesis of swine hemagglutinating encephalomyelitis virus for targeting neurons and estabishing persistent infection

Research Project

Project/Area Number 13660314
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionIWATE UNIVERSITY

Principal Investigator

HIRANO Norio  IWATE UNIV. FACULTY OF AGRICULTURE, Associate Professor, 農学部, 助教授 (40092308)

Co-Investigator(Kenkyū-buntansha) TOHYAMA Koujiro  IWATE MED. UNIV. SCH. OF MEDICINE, Associate Professor, 医学部, 助教授 (10129033)
TAIRA Hideharu  IWATE UNIV. FACULTY OF AGRICULTURE, Professor, 農学部, 教授 (70045756)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordscoronavirus / hemaggutinating encephalomyelitis virus / neurotropic / trans-synaptic pathway / persistent infection / CNS / trans-synaptic tracer / neuroscience / 血球凝集性脳脊髄炎ウイルス / 神経細胞親和性 / グリア細胞 / 軸索 / 坐骨神経
Research Abstract

I. In mice inoculated by various routes, the routes of infection, in descending order if efficacy, were intracerebral, intranasal, subcutaneous, intraperitoneal, intravenous and oral. In mice inoculated by subcutaneous route in hind leg, viral antigen was detected immunohistochemically in neurons of the lumbar spinal cord, cerebral cortex, and in Purkinje cells of cerebellum.
II. The virus spread was detected in the regenerating axons but not in the Schwann cells of peripheral nerves of rats, suggesting that the virus might spread from peripheral nerves to the CNS trans-synaptically as same as rabies virus.
III. The infected cells were identified immunohistochemically as neurons by double or triple staining using anti-HEV antibody with anti-GFAP (astrocytes) antibody or TB-4 (microglial) antibody.
IV. From these results, 1) the virus spreads in axons trans-synaptically from peripheral nerve to the CNS, 2) the Virus-targeting cells are neurons, 3) the infected neurons appeared to be non-cytopathological. 4) the cellular responses were not detected in the CNS.
These neurotropic properties of HEV are responsible for persistent infection, virus-travelling from peripheral nerves to the CNS by trans-synaptic pathways.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Hirano, N., Tohyama, K., Taira, H.: "Swine coronavirus (HEV) as a transneuronal tracer for analysing polysynaptic pathways in the CNS of laboratory animals"Animal Research and Welfare. Lavoratory Animals. 41-42 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, N., Sada, F., Haga, S., Tohyama, K.: "Susceptibility of rats of different ages to inoculation with swine hemaggluti-Nating encephalomyelitis virus (coronavirus) by various routes"Journal Comparative Pathology]. 125. 8-14 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaguchi et al.: "Immunohistochemical study of age-dependant brain lessions in mice infected Intranasally with Kasba (Chuzan) virus"Journal Comparative Pathology]. 124. 36-45 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, N., Tohyama, K., Taira, H., Hashikawa, T.: "Spread of hemagglutinating encephalomyelitis virus in the CNS of rats by Intransal route"Advances for Experimental Biology and Medicine. 494. 127-132 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, N., Ootani, N., Tohyama, K., Hashikawa, T: "Infection of hemagglutinating encephalomyelitis virus aat the visual pathways of rats"Advances for Experimental Biology and Medicine. 494. 149-154 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, N., Nomura, R., Tawara, T.: "Neurotropism of Haemagglutinating encephalomyelitis virus (coronavirus) in mice depending upon host age and route of infection"Journal of Comparative Pathology. (accepted). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, N., Sada, F,. Haga, S., Tohyama, K.: "Susceptibility of rats of different ages inoculation with swine Haemagglutinating encephalomyelitis virus (coronavirus) by various routes"J. Comp. Pathol.. 125(1). 8-14 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yamaguchi, R., et al.: "Immunohistochemical study of age-dependantbrain lesions in mice infected intracerebrally with Kasba (Chuzan) virus"J. Comp. Pathol.. 124. 36-45 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, N., Tohyama, K., Taira, H., Hashikawa, T.: "Spread of hemagglutinating encephalomyelitis virus in the CNS of rats by intranasal route"Adv. Exp, Med. Biol.. 494. 127-132 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, N., Ootani, N., Tohyama, K., Hashikawa, T.: "Infection of hemagglutinating encephalomyelitis virus at the visual pathywas of rats"Adv. Exp, Med. Biol.. 494. 149-154 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, N., Nomura, R., Tawara, T., Tohyama, K.: "Neurotropism of swine Haemagglutinating encephalomyelitis virus (coronavirus) in mice depending upon Host age and route of infection"J. Comp. Pathol.. (Accepted). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, N., Tohyama, K., Taira. H.: "Swine coronavirus (HEV) as a transneuronal tracer for analysing polysynaptic pathways in the CNS of laboratory animals ; In : Animal Resarch and Welfare"Laboratory Animals. Ltd. London. 41-42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirano, N., Tohyama, K., Taira, H.: "Swine coronavirus (HEV) as a transneuronal tracer for analysing polysynaptic pathways in the CNS of laboratory animals"In : Animal Research and Welfare. Laboratory Animals. 41-42 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirano, N., Sada, F., Haga, S., Tohyama, K.: "Susceptiblity of rats of different ages to inculation with swine hemaggluti-Nating encephalomyelitis virus (coronavirus) by various routes"Journal Comparative Pathology. 125. 8-14 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yamaguchi et al.: "Immunohistochemical study of age-dependant brain lesions in mice infected Intranasally with Kasba (Chuzan) virus"Journal Comparative Pathology. 124. 36-45 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirano, N., Tohyama, K., Taira, H., Hashikawa, T.: "Spread of hemagglutinating encephalomyelitis virus in the CNS of rats by Intransal route"Advances for Experimental Biology and Medicine. 494. 127-132 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirano, N., Ootani, N., Tohyama, K, Hashikawa, T: "Infection of hemagglutinating encephalomyelitis virus aat the visual pathways of rats"Advances for Experimental Biology and Medicine. 494. 149-154 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirano, N., Nomura, R., Tawara, T.: "Neurotropism of Haemagglutinating encephalomyelitis virus (coronavirus) in mice depending upon host age and route of infection"Journal of Comparative Pathology. (accepted). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirano, N., Tohyama, K., K, Taira, H.: "Swine coronavirus(HEV) as a transneural tracer for analysing polysynaptic pathways In the CNS of laboratory animals. p 41-42"In Animal Research and Welfare. A Partnership. Lab. Anim. Ltd. London(2001). 41-42 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamaguchi, R, et. al.: "Immunohistochemical study of age-dependant brain lesions in mice infected Intranasally with Kasba(Chuzan) virus"J. Comp. Pathol. 124(1). 36-45 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirano, N., et al.: "Susceptibility of rats of different ages to inoculation with swine hemagglutinating encephalomyelitis virus (a coronavirus) for rats"J. Comp. Pathol. 125(1). 8-14 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirano, N., Tohyama, K., K, Taira, H.: "Spread of hemagglutinating encephalomyelitis virus in the CNS of rats by intranasal route"Adv. Exp. Mcd. Biol. 494. 127-132 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirano, N., Ootani, N., Tohyama, K.: "Infection of hemaagglutinating encephalomyelitis virus (HEV) at the visual pathways of rats"Adv. Exp. Med. Biol. 494. 149-154 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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