• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Real-time bioimaging of the cellular function of an actin-regulatory protein, gelsolin

Research Project

Project/Area Number 13670001
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

FUJITA Hisakazu  Hokkaido Univ., Institute for Genetic Medicine, Instructor, 遺伝子病制御研究所, 助手 (30212187)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsGelsolin / GFP-fusion protein / Bioimaging / Fluorescence Resonance Energy Transfer : FRET
Research Abstract

Gelsolin, an actin-regulatory protein has at least three different activities, nucleating, severing and capping. These activities are tightly regulated by calcium ions (Ca^<2+>), pH, and polyphosphoinositides. Gelsolin regulates actin dynamics in cells by interaction with both filamentous (F-) and monomeric (G-) actins and controlling the length of actin polymers through a variety of mechanisms. In this study, we sought to obtain an image of spatio-temporal activation of gelsolin in the living cells by means of FRET (Fluorescence Resonance Energy Transfer).
For bioimaging of gelsolin activities in cells, mouse gelsolin cDNA were inserted between ECFP and EYFP, derivatives of green fluorescence protein, to create a probe (CGY) for FRET, and then the mammalian expression vector for CGY was constructed. The CGY expression vector was transfected in fibroblast derived from gelsolin knock-out mice and expression of CGY was confirmed by western blot analysis. The expression of CGY in G- cells resulted in increased rate of cell crawling, indicating CGY, chimeric gelsolin protein was functional in cells. Fluorescence intensity of EYFP was decreased at cell peripheral, whereas it was higher around nulei, suggesting FRET was occurred at the central region of cells, and gelsolin was activated at the edge of cells, because FRET was lost.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] N.Sagawa, et al.: "Gelsolin suppresses tumor igenicity through Inhibiting a PKC activation in lung cancer cell line"Br. J. Cancer. 88. 606-612 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Furukawa, et al.: "Identification of a novel gelsolin truncate in the vertical and metastatic phase malignant melanomas"Melanoma Res.. 12. 523-528 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Sazawa, et al.: "Adenovirus-mediated gelsolin gene therapy for orthotopic human urinary bladder cancer in nude mice"J. Urol.. 168. 1182-1187 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N. Sagawa, et al.: "Gelsolin suppresses tumor igenicity through Inhibiting a PKC activation in lung cancer cell line"Br. J. Cancer. 88. 606-612 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Furukawa, et al.: "Identification of a novel gelsolin truncate in the vertical and metastatic phase malignant melanomas"Melanoma Res.. 12. 523-528 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Sazawa, et al.: "Adenovirus-mediated gelsolin gene therapy for orthotopic human urinary bladder cancer in nude mice"J. Urol.. 168. 1182-1187 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N.Sagawa: "Gelsolin suppresses tumor igenicity through Inhibiting a PKC activation in lung cancer cell line"Br. J. Cancer. (in press).

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Furukawa: "Identification of a novel gelsolin truncate in the vertical and metastatic phase malignant melanomas"Melanoma Res.. 12. 523-528 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] A.Sazawa: "Adenovirus-mediated gelsolin gene therapy for orthotopic human urinary bladder cancer in nude mice"J. Urol.. 168. 1182-1187 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Fujita H.: "Gelsolin function as a metastasis suppressor and reguirement of the carboxyl-terminus for its effect"International Journal of Cancer. 93. 773-780 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi