| Project/Area Number |
13670002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tohoku University |
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Principal Investigator |
ITOH Tsunetoshi Tohoku Univ., School of Medicine, Professor, 大学院・医学研究科, 教授 (90004746)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGISHI Mai Tohoku Univ., School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (90332501)
SOGA Hiroyuki Tohoku Univ., School of Medicine, Research Associate, 大学院・医学系研究科, 助手 (20282121)
NAKAMURA Masanori Showa Univ., School of Dentistry, Professor, 歯学部, 教授 (50180394)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Thymus / Thymocytes / Cell sorter / Selection / Differentiation / Surface marker / Gene expression / TCR / セルソーティング / 同定 |
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| Research Abstract |
Thymocytes, derived from hematopoietic stem cells in the bone marrow, proliferate and differentiate into mature T cells after entering the thymus. One of the biggest problems for thymocyte differentiation is that the accurate selection mechanism is still unresolved. How T cell receptor is closely and chronologically interrelated with "the selection process", how self-reactive clones are eliminated, and how CD4 and CD8 are differentially expressed, are still uncertain.
To address these issues, we believe that the important key should be the isolation and identification of the subset about to go through the selection process. In this project, experiments were designed to analyse gene expression in subsets just before or after the selection. On the other hand, since, we realized surface markers and the computer application capable of analyzing their expression are not sufficient and not well coordinated, we undertook the substantial improvement of the analyzing program. With this renewed application, we could identify several subsets not noticed so far but may have apparent significance in thymocyte differentiation.
A fundamentally new pathway for thymocyte differentiation is now being progressively constructed, and the findings would be an important infomation to delineate and identify thymocyte subsets about to enter the critical selection process.
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