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Optical mapping of neuronal activities in the submucous plexus with single cell resolution

Research Project

Project/Area Number 13670036
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionTokyo Medical and Dental Universily

Principal Investigator

SAKAI Tetsuro  Tokyo Medical and Dental University, Graduate School and Faculty of Medicine, Associate Professor, 大学院・医歯学総合研究科, 助教授 (40153845)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordselectrical activity / submucous plexus / optical recording / voltage-sensitive dye / ganglion / styryl
Research Abstract

In order to identify functional units of the neural network within the submucous plexus of the guinea pig, multiple-site optical recording of transmembrane voltage activity (MSORTV) with single cell resolution, using a voltage-sensitive styryl dye (di-8-ANEPPS) and a 464 element photodiode array, were employed to monitor the spontaneous electrical activity of the neurons of which the separate ganglia in the plexus are composed. Spike sorting analysis of the raw optical data is used to identify the active neurons and isolate their individual spike trains. Computer generated animations are used to show the patterns of neuronal activity. Preliminary observations of quasicoincident bursts recorded from neurons in separate ganglia suggest the existence of supraganglionic neuronal networks, which form the functional units of the submucous plexus.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Sakai, T.: "Optical mapping approaches to cardiac electrophysiological functions"Japanese Journal of Physiology. 51(1). 1-18 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai, T.: "Optical imaging of the spatiotemporal patterns of the_excitation_spread_in_the_atrium_using a voltage-sensitive dye"Japanese Journal of Physiology. 51(suppl.). S49 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai, T.: "In vitro model of atrial arrythmia : optical mapping of tachycardia-like excitation in the rat atrium"Japanese Journal of Physiology. 52(suppl.). S73 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai, T.: "Optical mapping of excitation spread during tachycardia-like excitation in the isolated rat atrium"American Journal of Physiology. (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai T., Kamino K.: "Optical mapping approaches to cardiac electrophysiological functions"Japanese Journal of Physiology. 51. 1-18 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai T.: "Optical imaging of the spatiotemporal patterns of the excitation spread in the atrium using a voltage-sensitive dye"Japanese Journal of Physiology. 51 (suppl.). S49 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai T.: "In vitro model of atrial arrythmia : optical mapping of tachycardia-like excitation in-the rat atrium"Japanese Journal of Physiology. 52 (suppl.). S73 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai T.: "Optical mapping of excitation spread during tachycardia-like excitation in the isolated rat atrium"American Journal of Physiology. submitted.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sakai, T.: "In vitro model of atrial arrhythmiai optical mapping of tachycardia-Like excitation in the rat atrium"Japanese Journal of Physiology. 52(Suppl). S73 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tetsuro Sakai: "Optical mapping approaches to cardiac electrophysiological functions"Japanese Journal of Physiology. 51. 1-18 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tetsuro Sakai: "Optical imaging of the spatioemporal patterns of the excitation spread in the atrium using a voltage-sensitive clye"Japanese Journal of Physiology. 51(suppl.). 549 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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