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Mechanisms of hibernation and cell protection during hypothermic condition in golden hamster

Research Project

Project/Area Number 13670106
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General pharmacology
Research InstitutionFukuyama University

Principal Investigator

SHIOMI Hirohito  Fukuyama University, Faculty of Pharmacy and pharmaceutical Sciences, Professor, 薬学部, 教授 (60025715)

Co-Investigator(Kenkyū-buntansha) OMOTO Mikiko  , 助手 (20309636)
TAMURA Yutaka  Fukuyama University, Faculty of Pharmacy and pharmaceutical Sciences, Assistant Professor, 薬学部, 助教授 (30217202)
NAKAMURA Akiriro  Fukuyama University, Faculty of Pharmacy and pharmaceutical Sciences, Professor, 薬学部, 教授 (70172393)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordshibernation / adenosine / β-endorphin / Thyrotropin-releasing hormone (TRH) / brown adipose tissue (BAT) / hypothermia / hyperthermia / neuroprotection factor / アドレナリンβ3受容体 / 冬眠調節機構 / アデノシンA1受容体 / アデノシンA2受容体 / オピオイド / オピオイドδ受容体
Research Abstract

It is known that golden hamster hibernates in cold environment. The hibernation of the hamster is classified into entrance stage, maintenance stage and arousal stage. In entrance stage, the body temperature (B.T.) of hamster lowers to almost environmental temperature, and the lowered B.T. continues in maintenance stage. Intracerebroventricular (i.c.v.) administrations of adenosine (ADO) and ATP produced hypothermia in hamster. The hypothermia produced by ADO and ATP suppressed by CPT, an adenosine A1 receptor antagonist. Moreover, i.c.v. administrated CHA, an adenosine A1 receptor agonist, lowered the B.T. to almost environmental temperature. I.c.v. administrated β-endorphin produced hypothermia in a dose-dependent manner. DAMGO, a selective μ-receptor agonist, also lowered the B.T. to almost environmental temperature. In entrance stage, administration (i.c.v.) of CPT produced uprise of the B.T., but did not in maintenance stage. Administration of naloxone in maintenance stage produced … More uprise of the B.T., but did not in entrance stage. The A1 receptor number of the hypothalamus in entrance stage decreased. These results suggest that adenosine regulates the B.T. of hamster in entrance stage via A1 receptor, and opioid regulates B.T. in maintenance stage via μ receptor.
During arousal from hibernation, the body temperature of hibernating hamster rapidly rises from 6 ℃ to 37 ℃. Thermogenesis in brown adipose tissue (BAT) is import component for the hyperthermia in the rodents including hamster. Thyrotropin-releasing hormone (TRH) produces the hyperthermia by increase of base metabolism. Therefore, we investigated the functional linkage of central TRH and BAT in the hyperthermia during arousal from hibernation. Intracerebroventricular (i.c.v.) administration of TRH produced hyperthermia in the hibernating hamster, and the hibernation was interrupted. In acute experiment, temperature of rectum and inter scapular BAT (iBAT) rose by the i.c.v. administration of TRH. The hyperthermic effect of TRH was suppressed by surgical denervation of iBAT and/or administration of SR59230 , a β3 adrenoceptor antagonist. I.c.v. administration of TRH caused increase of norepinephrine (NE) content and NE turnover rate in the iBAT. These results suggest that central TRH system and peripheral BAT system have functionally linked through the sympathetic nervous system, and it is play an important role in the hyperthermia during arousal from hibernation. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Shintani, M., Tamura, Y., Omoto, M., Nakamura, A., Shiomi, H.: "Central thermoregulating system of hibernation in golden hamster"The Japanease Journal of Pharmacology. 88 Suppl.I. 238 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura, Y., Hyodo, Y., Fukui, T., Omoto.M.Shiomi, H.: "Effects of hibernation-regulating substances on low temperature-induced cell death in cultured neurons"The Japanease Journal of Pharmacology. 88 Suppl.I. 246 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shintani, M., Tamura, Y., Monden, M., Nakamura, A., Shiomi, H.: "Thrmo-regulating system of hamster during entrans stage and meintenance stage of hibernation. -Role of central adenosine and opioids -"Journal of Pharmacological Sciences. 91 Suppl.I. 172 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura, Y., Yokoyama, M., Shintani, M., Monden, M., Nakamura, A., Shiomi, H: "Mechanism of hyperthermia during arousal from hibernation in hamster. -Functional linkage of central thyrotropin-releasing hormone (TRH) and brown adipose tissue (BAT)"Journal of Pharmacological Sciences. 91 Suppl.I. 172 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shintani, M., Tamura, Y., Omoto, M., Nakamura, A. and Shiomi, H.: "Central thermoregulating system of hibernation in golden hamster"The Japanese Journal of Pharmacology. Vol.88 suppl.1. 238 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura, Y., Hyodo, Y., Fukui, T., Omoto, M. and Shiomi, H.: "Effects of hibernation-regulating substances on low temperature-induced cell death in cultured neurons"The Japanese Journal of Pharmacology. Vol.88 suppl.1. 246 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shintani, M., Tamura, Y., Monden, M., Nakamura, A. and Shiomi, H.: "Thermo-regulating system of hamster during entrans stage and maintenance stage of hibernation - Role of central adenosine and opioids -"Journal of Pharmacology Sciences. Vol.91 suppl.1. 172 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura, Y., Yokoyama, M., Shintani, M., Monden, M., Nakamura, A. and Shiomi, H.: "Mechanism of hyperthermia during arousal from hibernation in hamster - Functional linkage of central thyrotropin-releasing hormone (TRH) and brown adipose tissue (BAT) -"Journal of Pharmacology Sciences. Vol.91 suppl.1. 172 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shintani, M., Tamura, Y., Monden, M., Nakamura, A., Shiomi, H.: "Thrmo-regulating system of hamster during entrans stage and meintenance stage of hibernation. -Role of central adenosine and opiolds -"Journal of Pharmacological Sciences. 91 suppl. I. 172 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tamura, Y, Yokoyarna, M., Shintani, M., Monden, M., Nakamura, A., Shiomi, H.: "Mechanism of hyperthermia during arousal from hibernation in hamster. -Functional linkage of central thyrotropin-releasing hormone (TRH) and brown adipose tissue (BAT) -"Journal of Pharmacological Sciences. 91 suppl. I. 172 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shintani, M., Tamura, Y., Omoto, M., Nakamura, A., Shiomi, H.: "Central thermoregulating system of hibernation in golden hamster"Japanease Journal of Pharmacology. 88・Suppl.I. 238 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tamura, Y., Hyodo, Y., Fukui, T., Omoto, M., Shiomi, H.: "Effects of hibernation-regulating substances on low temperature-in-duced cell death in cultured neurons"Japanease Journal of Pharmacology. 88・Suppl.I. 246 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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