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Mechanism of somite segmentation by bHLH transcription factors

Research Project

Project/Area Number 13670116
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

BESSHO Yasumasa  Institute for Virus Research Associate Professor, ウイルス研究所, 助教授 (70261253)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordssomite / segmentation / transcription factor / bHLH / oscillation / proteasome / Ubiquitination / molecular clock / bHLH因子 / ノックアウトマウス
Research Abstract

Somites, the metameric units of vertebrate embryos, are generated by periodical segmentation, which is regulated by a molecular clock. Hes7, a bHLH gene essential for somite segmentation, displays cyclic expression in the presomitic mesoderm (PSM) with 2 h cycles synchronized with somite formation. Hes7 is also essential for the oscillatory expression of the other gene such as Lunatic fringe (Lfng). It is likely that the cyclic expression of Hes7 is involved in the molecular clock in somite segmentation. Thus, we analyzed the mechanism of the oscillatory expression of Hes7.
We show that Hes7 protein is also expressed in a dynamic manner, which depends on proteasome-mediated degradation. Spatial comparison revealed that Hes7 and Lfng transcription occurs in the Hes7 protein-negative domains. Furthermore, Hes7 and Lfng transcription is constitutively up-regulated in the absence of Hes7 protein and down-regulated by stabilization of Hes7 protein. Thus, periodic repression by Hes7 protein is critical for the cyclic transcription of Hes7 and Lfng, and this negative feedback represents a molecular basis for the segmentation clock.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Hirata, H., et al.: "Hes1 and Hes3 regulate maintenance of the isthmic organizer and development of the mid/hindbrain"EMBO J.. 20. 4454-4466 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satow, T., et al.: "The bHLH gene hesr2 promotes gliogenesis in mouse retina"J. Neurosci.. 21. 1265-1273 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Bessho, Y., et al.: "Dynamic expression and essential functions of Hes7 in somite segmentation"Genes & Dev.. 15. 2642-2647 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue, T., et al.: "Math3 and NeuroD regulate amacrine cell fate specification in the retina"Development. 129. 831-842 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirata, H., et al.: "Oscillatory expression of the bHLH factor Hes1 regulated by a negative feedback loop"Science. 298. 840-843 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Bessho, Y., et al.: "Periodic repression by the bHLH factor Hes7 is an essential mechanism for the somite segmentation clock"Genes & Dev.. 17(In Press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirata, H., et al.: "Hes1 and Hes3 regulate maintenance of the isthmic organizer and development of the mid/hindbrain"EMBO J.. 20. 4454-4466 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Satow, T., et al.: "The bHLH gene hesr2 promotes gliogenesis in mouse retina"J. Neurosci.. 21. 1265-1273 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Bessho, Y., et al.: "Dynamic expression and essential functions of Hes7 in somite segmentation"Genes & Dev.. 15. 2642-2647 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue, T., et al.: "Math3 and NeuroD regulate amacrine cell fate specification in the retina"Development. 129. 831-842 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hirata, H., et al.: "Oscillatory expression of the bHLH factor Hes1 regulated by a negative feedback loop"Science. 298. 840-843 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Bessho, Y., et al.: "Periodic repression by the bHLH factor Hes7 is an essential mechanism for the somite segmentation clock"Genes & Dev.. 17(in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue, T., et al.: "Math 3 and NeuroD regulate amacrine cell fate specification in the retina"Development. 129. 831-842 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Hirata, H., et al.: "Oscillatory expression of the bHLE factor Hes1 regulated by a negative feedback loop"Science. 298. 840-843 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Inoue, T., et al.: "Math3 and NeuroD regulate amacrine cell fate specification in the retina"Development. 129. 831-842 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Bessho, Y., et al.: "Dynamic expression and essential functions of Hes7 in somite segmentation"Genes & Dev. 15. 2642-2647 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Inoue, C., et al.: "Math6, a bHLH gene expressed in the developing nervous system, promotes neuronal differentiation"Genes to Cells. 6. 977-986 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hirata, H., et al.: "Hes1 and Hes3 regulate maintenance of the isthmic organizer and development of the mid/hindbrain"EMBO J. 20. 4454-4466 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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