| Project/Area Number |
13670162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
IIJIMA Tatsuo University of Tsukuba, Institute of Basic Medical Sciences, Pathology, Lecturer, 基礎医学系, 講師 (40222799)
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| Co-Investigator(Kenkyū-buntansha) |
MORISHITA Yukio University of Tsukuba, Institute of Clinical Medicine, Pathology, Assistant Professor, 臨床医学系, 助教授 (20271562)
NOGUCHI Masayuki University of Tsukuba, Institute of Basic Medical Sciences, Pathology, Professor, 基礎医学系, 教授 (00198582)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Pulmonary adenocarcinoma / Invasion / Fibroblast / Matrix Metalloproteinase / MMP-2 / MMP-9 / Zymography / Eary cancer / 腺維芽細胞 / matrix metaloproteinase-2 / microdissection / supression subtractive hybridization / matrix metaloproteinase-2 (MMP-2) / 繊維芽細胞 / laser capture microdissection (LCM) / gelatin zymography / real time PT-PCR / 免疫細胞染色 / 活性化 / Gelatin zymography / Film in situ zymography / Macro array |
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| Research Abstract |
Small pulmonary adenocarcinoma can be classified on the basis if their histological characteritstics and prognosis, and when classified as such, the prognosis of replacing-type adenocarcinoma with active fibroblast proliferation is significantly worse than adenocarcinoma without fibroblast proliferation. I n order to clarify the biological mechanisms of the key to the morphological changes associated with active fibroblast proliferation, we examined the activities of matrix metalloproteinase (MMP)-2 and MMP-9, which are important enzymes in the stromal invasion by cancers. The active MMP-2 and MMP-9 content 40 pulmonary adenocarcinomas that were less than 20 mm in diameter was measured by the gelatin zymography method. The quantity of active MMP-2 in the pulmonary adenacarcinomas with active fibroblast proliferation was higher than in the pulomnary adenocarcinoma without active fibroblast proliferation (P<0.001), but there were no correlations between the histological features and the activation of MMP-9. The presence of active fibroblast proliferation in small pulmonary adenocarcinomas suggests that the cancer cells have acquired the ability to invade through the action of active MMP-2, and this is thought to be one of the reasons for the worse prognosis of pulmonary adenocarcinoma with active fibroblast proliferation
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