| Project/Area Number |
13670169
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Mie University |
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Principal Investigator |
YOSHIDA Toshimichi Mie University, Faculty of Medicine, Professor, 医学部, 教授 (80166959)
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| Co-Investigator(Kenkyū-buntansha) |
INADA Hiroyasu Mie University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (90283522)
YOSHIDA Kyoko Mie University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (00242967)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Tenascin-C / Cancer cells / Receptor |
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| Research Abstract |
In pathological tissues undergoing the remodeling, tenascin-C (TN-C) is re-expressed. Breast cancer cases highly expressing TN-C show poorer prognosis of the patients. In vitro studies have demonstrated that TN-C promotes proliferation and migration of the cancer cells. However, it has been not yet clarified domains of TN-C have the promotive function and receptors of cancer cells bound to TN-C. In this project, we, first, prepared recombinant proteins of each domains of TN-C and showed that the alternative spliced domain of fibronection type III repeats (FN III) enhances cell proliferation and cell migration. Using a monoclonal antibody aginst the domain, we demonstrated that TN-C splice variants including the domain were expressed in the invading sites and that the expression was related to the cell proliferation in human samples of breast cancer tissues. Next, we analyzed the binding proteins by two-hybrid system using cDNA encoding the domain and a HeLa cell library. Aprotein having 7 trans-membrane domains was found and we confirmed that the protein bound to the FNIII domain in vitro. We produced an antibody to the binding protein in a rabbit and are now affinity-purifying the antibody. We will analyze the distribution of the protein in breast cancer tissues.
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