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Cartilage Tissue Engineering Using Chondrogenic Cell Line N1511

Research Project

Project/Area Number 13670231
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionAichi Medical University

Principal Investigator

WATANABE Hideto  Institute for Molecular Science of Medicine Associate Professor, 分子医科学研究所, 助教授 (90240514)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordscartilage / chondrocyte / cell line / differentiation / tissue engineering / p53 / knockout mouse / アグリカン / バーシカン / プロテオグリカン / 細胞外マトリックス / 再生医学
Research Abstract

The aim of the project was to develop a method of the cartilage tissue engineering using a chondrogenic cell line N1511, which had been established from chondrocytes of a p53-null mouse.
First, we characterized N1511 cells. Induction of chondrocyte differentiation was attained when the cells were treated with bone morphogenetic proteins (BMPs) and insulin, or parathyroid hormone and dexamethasone. Recovery of p53 expression in N1511 cells inhibited cell proliferation, suggesting that the cell proliferation could be regulated with p53 in this cell line. These results indicate that N1511 undergoes multiple steps of chondrocyte differentiation and because proliferation could be regulated by expression of p53, N1511 could be an excellent model for studies of chondrogenesis, the function of p53, and genetic engineering of cartilage tissue. We are currently studying the roles of various proteoglycans in chondrocyte differentiation, using this cell line.
We also performed two studies related to this project: The effect of ultrasound on chondrocyte differentiation of mesenchymal stem cells, and characterization of an anti-decorin mouse monoclonal antibody 6B6. The results of these studies will facilitate studies on chondrocyte differentiation and cartilage tissue engineering.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Kamiya, N.: "Establishment of a novel chondrocytic Cell line N1511 derived from P53-null mice"J Bone Miner Res. 17. 1832-1842 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sawada, H.: "Characterization of an anti-decorin monoclonal antibody, and its utility"J Biochem. 132. 997-1002 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 蛯沢 克己: "軟骨再生、特集「バイオマテリアルと再生医療」"バイオマテリアル(日本バイオマテリアル学会誌). 21(印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 渡辺 秀人: "プロテオグリカン不全マウス"細胞. 34. 64-67 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 渡辺 秀人: "軟骨マトリックスの機能と遺伝子異常"細胞. 35. 122-125 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 渡辺 秀人: "ポストゲノム時代の糖鎖生物学がわかる(わかる実験医学シリーズ)"羊土社. 131 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kamiya, N., et al.: "Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice"J Bone Miner Res. 17-10. 1832-1842 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sawada, H., et al.: "Characterization of an anti-decorin monoclonal antibody, and its utility"J Biochem. 132-6. 997-1002 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ebisawa, K., et al.: "Cartilage tissue engineering"Biomaterial. in press. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Watanabe, H., and Kimata, K.: "Knockout mice of proteoglycans"The Cell. 34-2. 64-67 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Watanabe, H.: "Cartilage matrix: Function and genetic diseases"The Cell. 35-4. 122-125 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Watanabe, H. and Kimata, K., Ed. Taniguchi, N.: "Structure and function of proteoglycans, in Glycobiology in post-genome era"Yodosha. 53-59 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kamiya, N: "Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice"J Bone Miner Res. 17・10. 1832-1842 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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