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Protective immune mechanisms of Mongolian jirds (Meriones unguiculatus) against infectious diseases with a special reference to the mucosal defense mediated by IgA

Research Project

Project/Area Number 13670240
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionHirosaki University

Principal Investigator

SATO Hiroshi  Hirosaki University School of Medicine, Department of Parasitology, Lecturer, 医学部, 講師 (90211945)

Co-Investigator(Kenkyū-buntansha) INABA Takashi  Hirosaki University School of Medicine, Department of Parasitology, Associated Professor, 医学部, 助教授 (60003612)
KAMIYA Haruo  Hirosaki University School of Medicine, Department of Parasitology, Professor, 医学部, 教授 (70002079)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsMongolian jird (Meriones unguiculatus) / Laboratory model / Monoclonal antibody / Immune mechanism / IgA / Parasitic disease / Passive immunity / Dendritic cells / 腸管免疫 / 感染防御 / 排虫 / lgA
Research Abstract

To assess the potential role of IgA antibody in expulsion of the nematode from the intestine, a panel of IgA monoclonal antibodies (mAbs) were produced from the mesenteric lymph nodes of BALB/c mice orally vaccinated with irradiated muscle larvae of Trichinella britovi. One IgA mAb, HUSM-Tb1, formed immunoprecipitates on the surface of live muscle larvae, and by immunohistochemistry reacted with their stichocytes and cuticular surface. Intraperitoneal injection of BALB/c mice with this mAb 5 hours before challenge conferred a high level of protection (more than 95%) against Trichinella infection, when 2.0 mg of specific IgA per 20 g body weight was given to a mouse. The same treatment produced a similar effect in SCID mice and rats, but not in Mongolian jirds (Meriones unguiculatus). In vivo clearance of intraperitoneally-injected IgA mAb was different between mice and jirds. In mice, more than a half of detectable fecal IgA was excreted within 5 hours after injection, and the excretio … More n was almost completed within 12 hours. In contrast, jirds excreted more then a half of detectable fecal IgA between 5 and 12 hours after injection, and completed its excretion by 24 hours. Correlating with it, prolonged retention of more IgA in the blood was noticed in jirds than mice. It is speculated that jirds might have some defect(s) in mucosal transportation of murine IgA, and it is needed to explore more on the mucosal IgA defense system unique to jirds.
Along with the works mentioned above, we have performed the following observations to clarify the characteristics of jird immune systems; 1) complement activity using the complement-dependent anti-Thy-1 glomerulonephritis model, 2) T-cell dependent elimination of intestinal cestode (Taenia crassiceps) infection and blood trypanosome (Trypanosoma grosi) infection, by use of T-cell-depleted jirds using anti-jird T-cell mAb (HUSM-M.g.15), 3) In vivo interaction of jird cutaneous dendritic cells with skin-penetrating parasites (Schistosoma mansoni) by use of anti-jird MHC class II mAb (HUSM-M.g.30), and 4) characterization of two IgG subclasses in jirds and temporal changes in humoral immune responses of T-cell-depleted, T. grosi-infected jirds. These works increase our knowledge on the unique immune defense system of Mongolian jirds that are highly useful as a laboratory model of various infectious diseases of medical importance. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Sato H, Chisty MM, Kamiya H: "Anti-Thy-1 monoclonal antibody-induced glomerulonephritis in Mongolian gerbils"Comparative Medicine. 50. 603-608 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato H, Ihama Y, Kamiya H: "Survival of destrobilated adults of Taenia crassiceps in T-cell-depleted Mongolian gerbils"Parasitology Research. 86. 284-289 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato H, Chisty MM, Nargis M, Inaba T, Yagisawa M, Kamiya H: "Monoclonal antibodies reactive with dendritic cells of Mongolian gerbils"Comparative Medicine. 51. 234-238 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato H, Kamiya H: "Defect of protective immunity to Schistosoma mansoni infection in Mongolian gerbils involves limited recruitment of dendritic cells in the vaccinated skin"Parasite Immunology. 23. 627-632 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chisty MM, Nargis M, Sato H, Inaba T, Takahashi G, Kamiya H: "Schistosoma mansoni : kinetics of glomerulonephritis in Mongolian gerbils and its correlation with intensity and duration of infection"Parasite. 9. 143-151 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inaba T, Sato H, Kamiya H: "Monoclonal IgA antibody-mediated expulsion of Trichinella from the intestine of mice"Parasitology. 126(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inaba T, Sato H, Kamiya H: "Impeded establishment of the infective stage of Trichinella in the intestinal mucosa of mice by passive transfer of an IgA monoclonal antibody"Journal of Veterinary Medical Science. 65(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato H, Ishita K, Matsuo K, Inaba T, Kamiya H, Ito M: "Persistent infection of Mongolian jirds with a non-pathogenic trypanosome, Trypanosoma (Herpetosoma) grosi"Parasitology. 126(in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato H, Chisty MM, Kamiya H: "Anti-Thy-1 monoclonal antibody-induced glomerulonephritis in Mongolian gerbils"Comparative Medicine. 50. 603-608 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato H, Ihama Y, Kamiya H: "Survival of destrobilated adults of Taenia crassiceps in T-cell-depleted Mongolian gerbils"Parasitology Research. 86. 284-289 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato H, Chisty MM, Nargis M, Inaba T, Yagisawa M, Kamiya H: "Monoclonal antibodies reactive with dendritic cells of Mongolian gerbils"Comparative Medicine. 51. 234-238 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato H, Kamiya H: "Defect of protective immunity to Schistosoma mansoni infection in Mongolian gerbils involves limited recruitment of dendritic cells in the vaccinated skin"Parasite Immunology. 23. 627-632 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Chisty MM, Nargis M, Sato H, Inaba T, Takahashi G, Kamiya H: "Schistosoma mansoni: kinetics of glomerulonephritis in Mongolian gerbils and its correlation with intensity and duration of infection"Parasite. 9. 143-151 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inaba T, Sato H, Kamiya H: "Monoclonal IgA antibody-mediated expulsion of Trichinella from the intestine of mice"Parasitology. 126 (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inaba T, Sato H, Kamiya H: "Impeded establishment of the infective stage of Trichinella in the intestinal mucosa of mice by passive transfer of an IgA monoclonal antibody"Journal of Veterinary Medical Science. 65 (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sato H, Ishita K, Matsuo K, Inaba T, Kamiya H, Ito M: "Persistent infection of Mongolian jirds with a non-pathogenic trypanosome, Trypanosoma (Herpetosoma) grosi"Parasitology. 126 (in press). (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inaba, T.: "Monoclonal IgA antibody-mediated expulsion of Trichinella from the intestine of mice"Parasitology. 126(in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sato, H.: "Functional visualization of the excretory system of adult Schistonoma mansoni by the fluorescent marker resorufin"Parasitology. 125・6. 527-535 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Chisty, M.M.: "Schistosoma mansoni: kinetics of glomerulonephritis in Mongolian gerbils and its correlation with intensity and duration of infection"Parasite. 9・1. 143-151 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sato, H.: "Monoclonal antibodies reactive with dendritic cells of Mongolian gerbils"Comparative Medicine. 51・3. 534-538 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sato, H.: "Defect of protective immunity to Schistosoma mansoni infection in Mongolian gerbils involves limited recruitment of dendritic cells in the vaccinated skin"Parasite Immunology. 23・12. 627-632 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sato, H., Chisty, M., Nargis, M., Inaba, T., Yagisawa, M., Kamiya, H.: "Monoclonal antibodies reactive with dendritic cells of Mongolian gerbils"Comparative Medicine. 51・3. 534-538 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sato, H., Kamiya, H.: "Defect of protective immunity to Schistosoma mansoni infection in Mongolian gerbils involves recruitment of dendritic cells in the vaccinated skin"Parasite Immunology. 23・12. 627-632 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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