| Project/Area Number |
13670256
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Jikei University School of Medicine |
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Principal Investigator |
ASAO Makioka JIKEI UNIVERSITY SCHOOL OF MEDICINE, TROPICAL MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (90119850)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Entamoeba invadens / excystation / development / cytochalasin D / calcium ion / aphidicolin / protein kinase C / cysteine protease / 脱糞 / 後嚢子 / protein kinase C / phosphatidylinositol 3-kinase / Entamoeba in invadens / カルモデュリン / オリザリン / アクチン細胞骨格 / latrunculin A / jasplakinolide / プロテアソーム / lactacystin |
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| Research Abstract |
We examined the effects of various inhibitors on the excystation and metacystic development of Entamoeba invadens to elucidate the mechanism for these processes. Among three actin-modifying drugs, latrunculin A and jasplakinolide inhibited the excystation and metacystic development, whereas cytochalasin D unexpectedly enhanced these processes. The dinitroaniline herbiside oryzalin, an inhibitor of microtubule assembly, inhibited both processes. The calcium chelators EGTA and EDTA inhibited the excystation. EGTA delayed metacystic development, while EDTA made it unusual. The calcium channel blocker bepridil inhibited both processes, associating with reduced cyst viability at higher concentrations. The calmodulin inhibitor trifluoperazine also inhibited the excystation and metacystic development. Aphidicolin, a specific inhibitor of the replicative DNA polymerases, inhibited these processes, following an inhibition of change in the expression of proteins during metacystic development. The protein kina~e C inhibitors staurosporine, chelerythrine and calphostin C inhibited the excystation and delayed the metacystic development. Wortmannin, a potent inhibitor of phosphatidylinositol 3-kinase, also inhibited these processes. The cysteine protease inhibitors Z-Phe-Ala-DMK and EST (E-64d) inhibited the excystation and metacystic development with no significant effect on cyst viability.
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