| Project/Area Number |
13670268
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Bacteriology (including Mycology)
|
|---|
| Research Institution | Hamamatsu University School of Medicine |
|---|
Principal Investigator |
KOIDE Yukio Hamamatsu University School of medicine, Department of Microbiology and Immunology, Professor, 医学部, 教授 (30126809)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
UCHIJIMA Masato Hamamatsu University School of Medicine, Department of Microbiology and Immunology, Assistant Professor, 医学部, 助手 (20252174)
NAGATA Toshi Hamamatsu University School of Medicine, Department of Microbiology and Immunology, Associate Professor, 医学部, 助教授 (90275024)
OHARA Naoya Nagasaki University Graduate School of Biomedical Sciences, Course of Medical and Dental Sciences, Division of Microbiology and Oral Infection, Associate Professor, 歯学部, 助教授 (70223930)
AOSHI Taiki Hamamatsu University School of Medicine, Department of Microbiology and Immunology, Assistant Professor, 医学部, 助手 (10324344)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
|
|---|
| Keywords | Mycobacterium tuberculosis / Epitopes / DNA vaccines / Algorism / T cells |
|---|
| Research Abstract |
The best vaccine available against tuberculosis (TB), BCG, gives variable protective efficacy. Therefore, there remains an urgent need for an improved vaccine. DNA vaccine is capable of inducing strong cellular immunity involved in the protection against TB. Therefore, DNA vaccine is a candidate to induce more effective protective immunity against TB. We, previously, observed that DNA vaccine expressing a single epitope is more potent in the induction of protective immunity than that encoding whole molecules. In this project, therefore, we tried to identify T-cell epitopes of M. tuberculosis antigens. As we demonstrated that MPB51 antigen is one of major protective antigens of M. tuberculosis. We attempted T-cell epitopes of MPB51 employing overlapping peptide library and computer algorism. Then, we found I-A^b-restricted CD4 T-cell epitopes in amino acid residues 171-190 and 191-210 and an H-2^b-restricted CD8 T-cell in residues 21-40. Furthermore, employing H-2 class I knockout, HLA-A^*0201-transgenic mice, we found an HLA-A^*0201-restricted CD8^+T-cell epitope of MPB51.
|
