|Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2002: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
In a previous study, we reported on a filamentous bacteriophage, f237, that is specifically associated wit the recent pandemic 03:K6 serovar strains of Vibrio parahaemolyticus. In this study, we examined distribution of the phage among clinical V. parahaemolyticus strains and characterized the phage itself. The followings are what we have obtained during this research term.
1. We examined the distribution of f237 among more recent clinical V. parahaemolyticus, and revealed that f237 is associated with not only 03:K6 serovar strains, but also other recent pandemic strains with serovar 04:K68 or 01:KUT. These results suggest that f237 is related to newly emerged pandemic strains of V. parahaemolyticus (Emerg. Infect. Dis. 7: 477-478, 2001).
2. We demonstrated that f237 is integrated into the host bacterial chromosome. Also we precisely determined the integration site of the phage (J. Bacteriol. 184: 4933-4935, 2002. Lancet 361: 743-749, 2003).
3. From the non-pandemic V. parahaemolyticus strains, we isolated two filamentou.s bacteriophages that are related to f237, and determined the whole genome sequences of the phages (manuscript in preparation).
4. We identified a new genetic marker of the pandemic V. parahaemolyticus strains (manuscript in preparation).