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Induction of adhesion molecules expression on vascular endothelial cells mediated by Bartonella species.

Research Project

Project/Area Number 13670278
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionKagoshima University

Principal Investigator

MAENO Nobuaki  Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (20305113)

Co-Investigator(Kenkyū-buntansha) MATAYOSHI Seiken  Kagoshima University, Graduate School of Medical and Dental Sciences, Assistant Professor, 大学院・医歯学総合研究科, 講師 (00128456)
YOSHIIE Kiyotaka  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (70174886)
ODA Hiroshi  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (40107868)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2003: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsBartonella species / cat scratch disease / endothelial cells / adhesion molecule / growth factor / cytokine / chemokine / emerging infectious disease / 人畜共通感染症 / Bartonella henselae / 人獣共通感染症
Research Abstract

1.B. henselae upregulated the adhesion molecules expression (ICAM-1, VCAM-1, and E-selectin) on human umbilical vein endothelial cells (HUVECs). The effect was abolished when B. henselae were separated from HUVECs by a filter membrane. The nonpiliated strain induced ICAM-1 expression to the same extent as the piliated strain. Inactivated B. henselae did not alter its stimulatory activity. Polymyxin B, which strongly inhibited the effect of LPS, did not exert any influence on the stimulatory activity. Some heat-stable component of B. henselae might bind to the endothelial cell surface, inducing adhesion molecules expression.
2.Viable and inactivated B. henselae dose-dependently induced chemokine (IL-8 and MCP-1) secretion in HUVECs. The time course of IL-8 and MCP-1 mRNA expressions were in accordance with those of protein production. Polymyxin B or boiling did not weaken the stimulatory effect of B. henselae. The induction of IL-8 and MCP-1 by B. henselae was partially inhibited by anti CD14 antibody. However, anti TLR-2 and 4 antibodies did not alter the chemokine production. Our results suggest that some heat-stable component of B. henselae may be responsible for the induction of IL-8 and MCP-1 in HUVECs by a CD14-dependent mechanism.
3.Purified B. henselae LPS showed lower activities in stimulating HUVECs compared with E. cob LPS.
4.We tried to isolate the endothelial cell stimulating factor from culture supernatant of B. henselae using ultrafiltration, gel filtration, and perfusion chromatography. As a result, the stimulating factor was estimated to be a protein of molecular weight about 30,000.
5.Viable and inactivated B. henselae inhibited the spontaneous apoptosis of endothelial cells and neutrophils in vitro. In addition, culture supernatant of B. henselae possessed the antiapoptotic activity. This factor seemed to be distinct from the stimulating factors for growth of HUVECs.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Nobuaki Maeno: "A heat-stable component of Bartonella henselae up-regulates intercellular adhesion molecule-1 expression on vascular endothelial cells"Scandinavian Journal of Immunology. 55. 366-372 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Song Mao: "CD 14-mediated induction of interleukin-8 and monocyte chemoattractant protein-1 by a heat-resistant constituent of Porphyromonas gingivalis in endothelial cells"Scandinavian Journal of Immunology. 56. 484-491 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuaki Maeno: "Highly elevated serum levels of interleukin-18 in systemic juvenile idiopathic arthritis but not in other juvenile idiopathic arthritis or in Kawasaki disease"Arthritis & Rheumatism. 46. 2539-2541 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Song Mao: "The induction of intercellular adhesion molecule-1 on human umbilical vein endothelial cells by a heat-stable component of Porphyromonas gingivalis"Current Microbiology. 48. 108-112 (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nobuaki Maeno: "Increased interleukin-18 expression in bone marrow of a patient with systemic juvenile idiopathic arthritis and unrecognized macrophage activation syndrome"Arthritis & Rheumatism. 50(In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 小田 紘: "現代感染症事情 ネコひっかき病"医歯薬出版 中山宏明,他(編). 298-311 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeno N, Yoshiie K, Matayoshi S, Fujimura T, Mao S, Wahid MR, Oda H: "A heat-stable component of Bartonella henselae up-regulates intercellular adhesion molecule-1 expression on vascular endothelial cells."Scandinavian Journal of Immunology. 55. 366-372 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mao S, Maeno N, Yoshiie K, Matayoshi S, Fujimura T, Oda H: "CD14 mediated induction of interleukin-8 and monocyte chemoattractant protein-1 by a heat-resistant constituent of Porphyromonas gingivalis in Endothelial cells."Scandinavian Journal of Immunology. 56. 484-491 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeno N, Takei S, Nomura Y, Imanaka H, Hokonohara M, Miyata K: "Highly elevated serum levels of interleukin-18 in systemic juvenile idiopathic arthritis but not in other juvenile idiopathic arthritis or in Kawasaki disease."Arthritis & Rheumatism. 46. 2539-2541 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mao S, Maeno N, Matayoshi S, Yoshiie K, Fujimura T, Oda H: "The induction of intercellular adhesion molecule-1 on human umbilical vein endothelial cells by a heat-stable component of Porphyromonas gingivalis."Current Microbiology. 48. 108-112 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Maeno N, Takei S, Imanaka H, Yamamoto K, Kuriwaki K, Kawano Y, Oda H: "Increased interleukin-18 expression in bone marrow of a patient with systemic juvenile idiopathic arthritis and unrecognized macrophage activation syndrome."Arthritis & Rheumatism. 50(in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Song Mao: "The induction of intercellular adhesion molecule-1 on human umbilical vein endothelial cells by a heat-stable component of Porphyromonas gingivalis"Current Microbiology. 48. 108-112 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nobuaki Maeno: "Increased interleukin-18 expression in bone marrow of a patient with systemic juvenile idiopathic arthritis and unrecognized macrophage activation syndrome"Arthritis & Rheumatism. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nobuaki Maeno: "Anti-agalactosyl IgG antibodies in juvenile idiopathic arthritis, juvenile onset Sjogren's syndrome, and healthy children"Journal of Rheumatology. in press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nobuaki Maeno: "Highly elevated serum levels of interleukin-18 in systemic juvenile idiopathic arthritis but not in other juvenile idiopathic arthritis or in Kawasaki disease"Arthritis & Rheumatism. 46. 2539-2541 (2002)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nobuaki Maeno: "A Heat-Stable Component of Bartonella henselae Up-Regulates Intercellular Adhesion Molecule-1 Expression on Vascular Endothelial Cells"Scandinavian Journal of Immunology. 55. 366-372 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mao Song: "CD14-Mediated Induction of Interleukin-8 and Monocyte Chemoattractant Protein-1 by a Heat-Resistant Constituent of Porphyromonas gingivalis in Endothelial Cells"Scandinavian Journal of Immunology. 56. 484-372 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nobuaki Maeno: "A Heat-Stable Component of Bartonella henselae Up-Regulates Intercellular Adhesion Molecule-1 Expression on Vascular Endothelial Cells"Scandinavian Journal of Immunology. (In press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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