Analysis of axonal transport of herpes simplex virus and examination of the possibility as a virus vector
| Project/Area Number |
13670291
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | Asahikawa Medical College |
|---|
Principal Investigator |
OGASAWARA Masahiro Asahikawa Medical College, Faculty of Medical science, Instructor, 医学部, 助手 (40185492)
|
|---|
| Project Period (FY) |
2001 – 2003
|
| Project Status |
Completed (Fiscal Year 2003)
|
| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2003: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
|---|
| Keywords | US3 / US11 / UL13 / UL15 / UL28 / UL34 / Dynein / Kinesin / ヘルペス / UL25 / Dynein IC1A / Dynein IC2C / DCTN1 |
|---|
| Research Abstract |
There are the microtubule-dependent motor protein dynein for retrograde axonal transport, the kinesin for anterograde transport. We were examined the interaction between those proteins and virus tegment proteins for the mechanism of axonal transport of herpes simplex virus (HSV). This examination suggested that the tegument protein UL34 has a high affinity for neuron-specific motor protein dynein IC1A variant, a low affinity for conventional dynein IC2C variant and cargo-adaptor protein DCTN1 (p150^<GIued>). To investigate the interactions In vivo, recombinant virus (rGFP-UL34) expressing a GFP-UL34 fusion protein was prepared. UL34 was located to nucleus and accumulated to nuclear membrane but not to microtubules in rGFP-UL34 infected-HeLa cells. Unfortunately, a UL34-expressed helper cells is necessary for the replication of rGFP-UL34. This result suggests that a GFP-UL34 fusion protein impaired a physical function in tegment protein and/or does not included in a virion.
A recent repo
… More
rt demonstrates an interaction between US11 and the ubiquitous kinesin as the other motor protein. We prepared the recombinant virus (rGFP-US11) expressing a GFP-US11 fusion protein to observe the anterograde axonal transport of HSV. US11 was expressed in the cytoplasm and located to nucleoli but not to microtubules in rGFP-US11 infected-HeLa cells. To investigate the participation of virus protein kinases (UL13 and US3) to the axonal transport, the following recombinant viruses was prepared ; rGFP-US11 virus, US3 deletion virus, UL13 deletion virus, US3 deletion/rGFP-US11 virus, UL13 deletion/rGFP-US11l virus. Unfortunately, the differences among these viruses-infected cells were not observed in the release of viruses to culture media. Since US11 is essential for axonal transport of HSV in vivo but not for productive viral growth in cultured cells, we are studying in vivo experiments such as the primary culture of ganglia cells or in vivo system of rodents. On the other hand, we investigate the packaging of virus genomes, especially cleavage mechanism for the formation of unit-length genomes. Less
|
Report
(4 results)
Research Products
(11 results)
