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Gene analysis of human parainfluenza virus type 4 and analysis of virus receptor

Research Project

Project/Area Number13670304
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionSuzuka University of Medical Science

Principal Investigator

KOMADA Hiroshi  Suzuka University of Medical Science, Faculty of Health Science, Professor, 保健衛生学部, 教授 (10144247)

Project Period (FY) 2001 – 2003
Project Status Completed(Fiscal Year 2003)
Budget Amount *help
¥3,600,000 (Direct Cost : ¥3,600,000)
Fiscal Year 2003 : ¥500,000 (Direct Cost : ¥500,000)
Fiscal Year 2002 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 2001 : ¥2,400,000 (Direct Cost : ¥2,400,000)
KeywordsParainfluenza virus type 4 / gene analysis / lymphocyte / stimulation / CD98HC / IL-2 / IL-2R / モノクローナル抗体 / リンパ球増殖 / パラインフルエンザウイルス4型 / transgenicマウス / HPIV-4 / Tgマウス / mitogenesis
Research Abstract

We analyzed the gene sequence of human parainfluenza virus type 4 L protein. More than 80% was finished, and only gene ending region was remaining.
CD98 is a widely expressed cell surface heterodimetric protein of 125kDa. Its expression is up-regulated during lymphocyte activation induced by mitogen, superantigen, conventional antigen, arid a combination of phorbol myristate acstate (PMA) and ionomycin. However, the role of CD98 in the immune system is not so well understood. The role of CD98 in lymphocyte proliferation was investigated, especially in correlation with IL-2/IL-2R system. Monoclonal antibody (mAb) (26-24) directed against murine CD98 heavy chain (mCD98HC) suppressed the proliferation of lymphocytes stimulated with concunavalin A (Con A). However, anti-CD98HC mAb (26-24) did not inhibit the production of interleukin 2 (IL-2) by lymphocyte stimulated with Coil A. In addition, IL-2 (recombinant), which induced considerable DNA synthesis of lymphocytes stimulated with sub-optimal dose of Con A, did not induce CD98HC expression in lymphocytes. Anti-mCD98HC mAb did not suress the expression of IL-2Rα. Anti-LL-2RαmAb, which suppressed DNA synthesis, did not inhibit the expression of CD98HC. Taken together with these findings, it was inferred that CD98 system is independent of IL-2-IL-2R system in lymphocyte activation.

Report

(4results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report

Research Products

(23results)

All Other

All Publications

  • [Publications] Tsumura H, Komada H.: "The targeted distribution of the CD98 gene results in emblionic lethality"BBRC. 308. 847-851 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishio M, Komada H.: "Characterization of Sendai virus persistently infected L929 cells and Sendai virus pi strain : recombinant Sendai virus having Mpi protein shows lower cytotoxicity and are incapable of establishing persistent infection"Virology. 314. 110-124 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kozuka Y, Komada H.: "Identification of amino acids essential for the human parainfluenza type 2 virus V protein to lower the intracellular levels of STAT2"Virology. 317. 208-214 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mori K., Komada H.: "The functional interactions between CD98 and CD147 in regulating virus-induced cell fusion and osteoclast formation"Med.Micro.Immunol.. (in press). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Komada H., Onoda T: "The role of CD98 heavy chain in lymphocyte proliferation and an attempt to make transgenic mice that have human CD98 heavy chain"Cell Struct Funct.. 28. 363-363 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Komada H., Hattori E.: "Tissue distribution of mouse CD98 heavy chain and role of CD98HC in lymphocyte proliferation"Cell Struct Funct. 27. 271-271 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yuji Kozuka, Yasufumi Yamashita, Mitsuo Kawano, Masato Tsurudome, Morihiro Ito, Machiko Nishio, Hiroshi Komada, Yasuhiko Ito: "Identification of amino acids essential for the human parainfluenza virus type 2 virus V protein to lower the intracellular levels of the STAT2."Virology. 317. 208-219 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Machiko Nishio, Masato Tsurudome, Morihiro Ito, Mitsuo Kawano, Hiroshi Komada, Yasuhiko Ito.: "Characterization of Sendai virus persistently infected L929 cells and Sendai virus pi strain : recombinant Sendai viruses having Mpi protein shows lower cytotoxicity arid are incapable of establishing persistent infection"Virology. 314. 110-124 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hideki Tsumura, Noboru Suzuki, Hiromitsu Saito, Mitsuo Kawano, Seiich Otake, Yuji Kozuka, Hiroshi Komada, Masato Tsurudome, Yasuhiko Ito: "The targeted disruption of the CD98 gene results in embryonic lethality"Biochemical and Biophysical Research Communications. 308. 847-851 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Morihiro Ito, Shin-ichiro Takebayashi, Katsuzumi Okumura, Takeshi Ohkubo, Machiko Nishio, Mitsuo Kawano, Hiroshi Komada, Yoshiro Ito, Masato Tusrudome: "Up-regulated expression of a novel gene in activated human peripheral blood mononuclear cells that is a truncated paralog of the human system L-amino acid transporter 1"The International Journal of Biochemistry and Cell Biology. 34. 1152-1163 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Machiko Nishio, Masato Tsurudome, Morihiro Ito, Mitsuo Kawano, Hiroshi Komada, Yasuhiko Ito: "High resistance of human parainfluenza type 2 virus protein-expressing cells to the antiviral and anti-cell proliferative"activities of alpha/beta interferons : cystein-rich V-specific domain is required for high resistance to the interferons Journal of Virology. 75. 9165-9176 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masato Tsurudome, Morihiro Ito, Machiko Nishio, Mitsuo Kawano, Hiroshi Komada, Yasuhiko Ito: "Hemagglutinin-neuraminidase-independent fusion activity of simian virus 5 fusion protein: difference in conformation between fusogenic and nonfusogenic F J)roteins on the cell surface."Journal of Virology. 75. 8999-9009 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mitsuo Kawano, Masahiko Kai to, Yuji Kozuka, Hiroshi Komada, Naoya Noda, Kazuyoshi Namba, Masato Tsurudome, Morihiro Ito, Machiko Nishio, Yasuhiko Ito.: "Recovery of infectious human parainfluenza type 2 virus from cDNA clones and properties of the defective virus without V-specific cystein-rich domain"Virology. 284. 99-112 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tsumura H, Komada H., Ito Y.et al.: "The targeted distribution of the CD98 gene results in emblionic lethality"BBRC. 308. 847-851 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nishio M, Komada H., Ito Y et al.: "Characterization of Sendai virus persistently infected L929 cells and Sendai virus pi strain : recombinant Sendai virus having Mpi protein shows lower cytotoxicity and are incapable of establishing persistent infection"Virology. 314. 110-124 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kozuka Y., Komada H., Ito Y et al.: "Identification of amimo acids essential for the human parainfluenza type 2 virus V protein to lower the intracellular levels of STAT2"Virology. 317. 208-214 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mori K., Komada H., Ito Y.et al.: "The functional interactions between CD98 and CD147 in regulating virus-induced cell fusion and osteoclast formation"Med.Micro.Immunol.. in press. (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Komada H., Onoda T., Xuramochi M.: "The role of CD98 heavy chain in lymphocyte proliferation and an attempt to make transgenic mice that have human CD98 heavy chain"Cell Struct Funct.. 28. 363-363 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Ito, H.Komada et al.: "Up-regulated expression of a novel gene in activated human peripheral blood mononuclear cells that is a truncated paralog of the human system L-amino acid transporter 1"The International Journal of Biochemistry and Cell Biology. 34. 1152-1163 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Komada, E.Hattori et al.: "Tissue distribution of mouse CD98 heavy chain and the role of CD98HC in lymphocyte"Cell Structure and Function. 27. 271-271 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishio M. et al.: "High resistance of human parainfluenza type 2 virus protein-expressing cells to the antiviral and anti-cell proliferative activities of alpha/beta interferons : cysteine-rich V-specific domain is required for high resistance to the interferons"J Vivol.. 75(19). 9165-9176 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsurudome M. et al.: "Hemagglutinin-neuraminidase-independent fusion activity of simian virus 5 fusion (F) protein : difference in conformation between fusogenic and nonfusogenic F proteins on the cell surface"J Vivol.. 75(19). 8999-9009 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kawano M. et al.: "Recovery of infectious human parainflueaza type 2 virus from cDNA clones and properties of the defective virus without V-specific cysteine-rich domain"Virology. 284(1). 99-112 (2001)

    • Related Report
      2001 Annual Research Report

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Published : 2001-04-01   Modified : 2016-04-21  

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