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Physiological role of human specific seminal ubiquitinated CD10-CD13 complex enzyme

Research Project

Project/Area Number 13670431
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionFUKUSHIMA MEDICAL UNIVERSITY

Principal Investigator

ABE Sumiko  Fukushima Medical University School of Medicine, Department of Legal Medicine, lecturer, 医学部, 助手 (50136975)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
KeywordsCD10-CD13 complex / γ-glutamyl transpeptidase active enzyme / human seman / HL60 / HeLa / mRNA / MBL
Research Abstract

Human semen-specific γ-glutamyl transpeptidase active protein (SEGAP) is a unique enzyme complex of ubiquitinated CD10 and CD13. In our previous study, we examined the potential of SEGAP to induce phosphorylation of tyrosine in HeLa (human cervical carcinoma) and HL60 (human promyelocytic leukemia) cells, and we observed phosphorylation of 110-kDa, 70-kDa and 60-kDa proteins that are thought to be PI3 kinase, Syk/ZAP-70 and Scr family proteins, respectively. These results suggested that SEGAP affects mRNA expressions of cytokines and other signals in HeLa and HL60 cells.
In this study, we investigated the changes in mRNA levels in SEGAP-treated HeLa and HL60 cells. IL-1β, IL-8, IL-12, TNF-a, Lyn, Fas and c-fos mRNAs were all expressed in HeLa cells, and their expression levels were not affected by SEGAP. Mannose-binding lectin (MBL) mRNAs were not expressed in control HeLa cells. On the other hand, SEGAP induced MBL mRNA expression in HeLa cells after 15 to 30 min of incubation. Since MBL initiates the lectin complement pathway that mediates the innate immune system, SEGAP may activate the defense system against invading microorganisms. SEGAP did not affect mRNA levels of IL-1β, IL-8, IL-12, TNF-a, Lyn or c-fos in HL60 cells. CT-1 and iNOS mRNAs were not expressed in control HL60 cells. In a manner similar to that in HeLa cells, SEGAP induced CT-1 and iNOS mRNA expressions in HL60 cells after 15 to 30 min of incubation. NO is one of the messenger molecules involved in the bacteria killing process. These results suggest that SEGAP induces lectin, CT-1 and iNOS mRNA expressions in order to protect sperm from invading microorganisms.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Abe S., et al.: "The effect of a tourniquet on NOS mRNA expression in a rat dorsal root ganglion"The proceedings of the 16^<th> Meeting of the International Association of Forensic Sciences. 85-89 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abe S., et al.: "Changes in mRNA expression patterns for cytokines in blood leukocytes of a rat tourniquet model"Legal Medicine. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mizusawa I., Abe S., et al.: "Expression of cytokines, neurotrophins, neurotrophin receptors and NOS mRNA in dorsal root ganglion of a rat tourniquet model"Legal Medicine. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abe S., et al.: "NOS mRNA expression in a rat dorsal root ganglion after application of a tourniquet to a hind limb"Journal of Forensic Medicine (16^<th> Meeting of the International Association of Forensic Sciences Abstract book). 45. 96 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mizusawa I., Abe S., et al.: "Expression of cytokines, Trk and NOS in dorsal root ganglia of a rat after application of a tourniquet to a hind limb"5^<th> International Symposium on Advances in Legal Medicine Abstracts. 112 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abe S., et al.: "Cytokine expression in blood leukocytes of a rat after application of a tourniuet to a hind llimb"5^<th> International Symposium on Advances in Legal Medicine Abstracts. 115 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sumiko Abe, Miwako Suto, Osamu Muraki, Mikio Kuraya, Teizo Fujita, Masahide Nakamura, Hirobumi Gunji, Kouichi Hiraiwa: "Immunohistochemical characterization of seminal γ-glutamyl transpeptidase active protein constituent-ubiquitin in prostate disease"Fukushima Medical Journal. 51. 189-197 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sumiko Abe, Miwako Suto, Kouichi Hiraiwa: "The effect of seminal γ-glutamyl transpeptidase active protein on tyrosine phosphorylation"Japanese Journal of Legal Medicine. 51, 65. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sumiko Abe, Ikubumi Mizusawa, Katsuyuki Kanno, Atsuko Yabashi, Takashi Honda, Miwako Suto, Mikio Kuraya, Kouichi Hiraiwa: "The effect of a tourniquet on NOS mRNA expression in a rat dorsal root ganglion"The proceedings of the 16^<th> Meeting of the International Association of Forensic Sciences. 85-89 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sumiko Abe, Ikubumi Mizusawa, Katsuyuki Kanno, Atsuko Yabashi, Takashi Honda, Miwako Suto, Mikio Kuraya, Kouichi Hiraiwa: "Changes in mRNA expression patterns for cytokines in blood leukocytes of a rat tourniquet model"Legal Medicine. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ikubumi Mizusawa, Sumiko Abe, Katsuyuki Kanno, Atsuko Yabashi, Takashi Honda, Miwako Suto, Kouichi Hiraiwa: "Expression of cytokines, neurotrophins, neurotrophin receptors and NOS mRNA in dorsal root ganglion of a rat tourniquet model"Legal Medicine. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Abe S., et al.: "The effect of a tourniquet on NOS mRNA expression in a rat dorsal root ganglion"The proceedings of the 16^<th> Meeting of the International Association of Forensic Sciences. 85-89 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Abe S., et al.: "Changes in mRNA expression patterns for cytokines in blood leukocytes of a rat tourniquet model"Legal Medicine. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizusawa I., Abe S., et al.: "Expression of cytokines, neurotrophins, neurotrophin receptors and NOS mRNA in dorsal root ganglion of a rat tourniquet model"Legal Medicine. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] Abe S., et al.: "NOS mRNA expression in a rat dorsal root ganglion after application of a tourniquet to a hind limb"Journal of Forensic Medicine (16^<th> Meeting of the International Association of Forensic Sciences Abstract book). 45. 96 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mizusawa I., Abe S., et al.: "Expression of cytokines, Trk and NOS in dorsal root ganglia of a rat after application of a tourniquet to a hind limb"5^<th> International Symposium on Advances in Legal Medicine Abstracts. 112 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Abe S., et al.: "Cytokine expression in blood leukocytes of a rat after application of a tourniuet to a hind llimb"5^<th> International Symposium on Advances in Legal Medicine Abstracts. 115 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 阿部すみ子: "前立腺病変による精液特異 γ-glutamyl transpeptidase活性酵素構成ユビキチンの免疫反応性の変化"福島医学雑誌. 51・3. 189-197 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 阿部すみ子: "下肢緊縛によるラット後根神経節細胞mRNA発現の変化"日本法医学雑誌. (印刷中).

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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