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Research for antifungal mechanisms and therapeutics in refractory deep mycoses

Research Project

Project/Area Number 13670462
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionNagasaki University

Principal Investigator

MIYAZAKI Yoshitsugu  Nagasaki University Hospital, Assistant professor, 医学部附属病院, 講師 (00311861)

Co-Investigator(Kenkyū-buntansha) KOHNO Shhigeru  Nagasaki Univ. Postgraduate Sch. Of Med Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (80136647)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsazole / candida albicans / sterol / treatment / virulence / filament / hyphe / fungus / Cryptococcus neoformans / アゾール系抗真菌薬 / ERG11 / 薬剤感受性 / アゾール耐性 / 深在性真菌症 / クリプトコッカス / 抗真菌薬
Research Abstract

We Sterols constituting cell membrane of Candida albicans, one of the most important opportunistic pathogen, play some important roles in causing disease in humans. Here, we created the C. albicans erg3 null mutant to describe effects of sterol dysbolism by defective delta-5, 6-desaturase on azole antifungal susceptibilities, pathogenicity and morphogenesis. The erg3/erg3 strain showed highly azole resistance and ergosta-7, 22-dien-3β-ol accumulated in place of ergosterol was not affected by fluconazole exposure. While, sterol dysbolism by defective delta-5, 6-desaturase increased vulnerability to Sodium dodecyl sulfate (SDS), reduced virulence in a mouse model of systemic candidiasis and suppressed filamentous growth on solid medium and in liquid culture without shaking. Our results are useful for further under standing linkage of multiple signaling pathways for morphogenesis.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Kaneko Y.: "Effects of parenterally administered ciprofloxacin in a murine model of pulmonary pseudomonas aeruginosa infection mimicking ventilator-associated pneumonia"Chemotherapy. 47. 421-429 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 掛屋 弘: "アゾール系抗真菌薬の標的酵素14α-Demethylaseの点変異の耐性機序への関与"化学療法の領域. 17. 125-132 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 平松啓一: "耐性菌感染症の理論と実際 第2版"医薬ジャーナル社. 281 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kaneko Y.: "Effects of parenterally administered ciprofloxacin in a murine model of pulmonary pseudomonas aeruginosa in fection mimicking ventilator-associated pneumonia"Chemotherapy. 47. 421-429 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hiroshi K: "Effect of mutation in 14 alpha-demthylase on resistant mechanism"Kagakuryoho-no-ryouiki. 17. 125-132 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kaneko Y.: "Effects of parenterally administered ciprofloxacin in a murine model of pulmonary pseudomonas aeruginosa infection mimicking ventilator-associated pneumonia"Chemotherapy. 47. 421-429 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 掛屋弘: "アゾール系抗真菌薬の標的酵素14α-Demethylaseの点変異の耐性機序への関与"化学療法の領域. 17. 125-132 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] 平松啓一: "耐性菌感染症の理論と実際 第2版"医薬ジャーナル社. 281 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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