Analysis of HLA-DRB1^*O901-binding HPV-16 E7 Helper T cell Epitope.
| Project/Area Number |
13670472
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Saitama Medical University |
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Principal Investigator |
OKUBO Mitsuo Saitama Medical School, Faculty of medicine, Assistant Professor, 医学部, 講師 (40260781)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Hiroo Saitama Medical School, Faculty of medicine, Professor, 医学部, 教授 (30134597)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2003: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | HLA-DR / T cell epitope / human papillomavirus / Th1, Th2 / cervical carcinoma / 子宮癌 / Th1 / Th2 / ヒトパピローマウイルス / HLA / T細胞 |
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| Research Abstract |
Objective : This study sought to determine the human papillomavirus(HPV)-16E7 epitopes that would be presented by HLA-DR molecules to CD4-positive T cells in patients with cervical carcinoma.
Methods : HLA-DR binding assays were performed using HPV-16 E7-derived synthetic peptides and, after incubation with these DR-binding peptides, helper T cell frequencies were analyzed in patients whose HLA and HPV genotypes were confirmed.
Results and Conclusion : We determined that the E7d peptide,^<61>CDSTLRLCVQSTHVDIRTL^<80>E, was bound by HLA-DRB1^*0901. An increased frequency(0.3-2.4%) of type 2 helper T cell responses was found in HLA-DRB1^*0901-positive patients with cervical dyaplasia and carcinoma. We found that when IL-12 was combined with E7d-peptide stimulation in vitro, the frequency of type 1helper T cell responses also increased in the patients with carcinoma. Thus HPV-16 E7d peptide as an HLA-DRB1^*0901-restricted helper T cell epitope might usefully be incorporated in understanding immunological mechanism and immunotherapy for this disease.
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Report
(4 results)
Research Products
(3 results)
