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Is gap junction the defensive factor against development, progression, and metastasis of gastric cancer?

Research Project

Project/Area Number 13670491
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionTokai University

Principal Investigator

MINE Tetsuya  Tokai University, School of Medicine, professor, 医学部, 教授 (20157572)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsGap junction / Intercellular communication / Cultured gastric cancer cells / Cultured gastric mucosal cells / Wnt signaling / WNT8B / FRAT2 / Connexin / lucifer yellow / dye transfer
Research Abstract

The role of gap junction in development ,progression and metastasis of gastric cancer was investigated in this project. First, gap junction formation was confirmed to be made during the culture and differentiation of gastric mucosal cells originated from rabbit fetus. Next, the expression of gap junction in the gastric cancer cell lines was examined and there was no expression during the culture and proliferation of the gastric cancer cell lines. To investigate the mechanism of progression and invasion of gastric cancer to the normal tissues, the expression of gap junction as well as proliferation was investigated using the coculture of normal gastric mucosal cells and gastric cancer cells. There was seen a gap junction between two cells and the proliferation activity of gastric cancer cells was decreased. At that time, it was considered that this project can be finished. Therefore, next, it was investigated how the expression of gap junction was regulated. Furthermore, it was confirmed that Wnt signaling including WNT8B and WNT10B was increased during the culture of gastric cancer cells. It seems that there is a reciprocal change between Wnt signaling and the expression of gap junction. Also, FRAT2, a mediator of Wnt signaling, was increased in parallel with proliferation of gastric cancer cells. From these data, it seems that the expression of gap junction might be regulated by Wnt signaling.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (45 results)

All Other

All Publications (45 results)

  • [Publications] Nagata J et al.: "Reversal of cisplatin and multidrug pesistance by ribozyme-mediated glutathione suppression"Biochem Biophys Res Commun. 286. 406-413 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T: "Proto-oncogenic WNT 10β is up-regulated by tumor necrosis factor α in human gastric cancer cell line MKN45"Inter J Oncol. 19. 1187-1192 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsukawa Y et al.: "Sero prevalence of Helicobacter pylon after bone marrow transplantation"Int J Immunotherapy. 17. 21 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T: "Up-regulation of Frezzled-10(FZD10) by β-estradiol in MCF-7 cell and by retinsic acid in NT_2 cells"Inter J Oncol. 20. 117-120 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasumi K et al.: "The roles of tumor necrosis factor α receptor type 1 and Fas in the Helicobacter pylori-induced apoptosis of gastric epithelial cells"J Gastroenterol Hepatol. 17. 651-658 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takayama I et al.: "The interstitial cells of Cajal and a gastroenteric pacemaker system"Arch Histol Cytol. 65. 1-26 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai K et al.: "Zonal difference in inhibition of DNA synthesis caused by anesthetics in cultured rat hepatocytes"Jap J Anesth. 51. 505-508 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagata J et al.: "Reversal of drug resistance using hammerhead ribozymes against multidrug resistance-associated protein and multidrug resistance 1 gene"Int J Oncol. 21. 1021-1026 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagata J et al.: "Angiopoietin-1 and vascular endothelial growth factor expression in human esophageal cancer"Int J Mol Med. 10. 423-426 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagata J et al.: "Correlation between interleukin 10 and vascular endothelial growth factor expression in human esophageal cancer"Int J Mol Med. 10. 169-172 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T et al.: "Up-regulation of WNT8B mRNA in human gastric cancer"Int J Oncol. 20. 343-348 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T et al.: "Molecular cloning and characterization of human GIPC3, a novel gene homologous to human GIPC1 and GIPC2"Int J Oncol. 20. 577-582 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T et al.: "Molecular cloning and characterization of mouse Gipc3"Int J Mol Med. 20. 251-256 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T et al.: "Molecular cloning and expression of proto-oncogene FRAT1 in human cancer"Int J Oncol. 20. 785-789 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T et al.: "Expression and regulation of WNT8A and WNT8B mRNAs in human tumor cell lines : up-regulation of WNT8B mRNA by beta-estradiol in MCF-7 cells, and down-regulation of WNT8A and WNT8B mRNAs by retinoic acid in NT2 cells"Int J Oncol. 20. 999-1003 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagata J et al.: "Reversal of cisplatin and multidrug resistance by ribozyme-mediated glutathione suppression"Biochem Biophys Res Commun. 286. 406-413 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T: "Proto-oncogenic WNT 10β is up-regulated by tumor necrosis α in human gastric cancer cell line MKN45"Inter J Oncol. 19. 1187-1192 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Matsukawa Y et al.: "Sero prevalence of Helicobacter pylori after bone marrow transplantation"Int J Immunotherapy. 17. 21 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T: "Up-regulation of Frezzled-10(FZD10) by β-estradiol in MCF-7 cell and by retinsic acid in NT_2 cells"Inter J Oncol. 20. 117-120 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Hasumi K et al: "The roles of tumor necrosis factor α receptor type 1 and Fas in the Helicobacter pylori-induced apoptosis of gastric epithelial cells"J Gastroenterol Hepatol. 17. 651-658 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takayama I et al: "The interstitial cells of Cajal and gastroenteric pacemaker system"Arch Histol Cytol. 65. 1-26 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Imai K et al: "Zonal difference in inhibition of DNA synthesis caused by anesthetics in cultured rat hepatocytes"Jap J Anesth. 51. 505-508 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagata J et al: "Reversal of drug resistance using hammerhead ribozymes against multidrug resistance-associated protein and multidrug resistance 1 gene"Int J Oncol. 21. 1021-1026 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagata J et al: "Angiopojetin-1 and vascular endothelial growth factor expression in human esophageal cancer"Int J Mol Med. 10. 423-426 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nagata J et al: "Correlation between interleukin 10 and vascular endothelial growth factor expression in human esophageal cancer"Int J Mol Med. 10. 169-172 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T et al: "Up-regulation of WNT8B mRNA in human gastric cancer"Int J Oncol. 20. 343-348 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T et al: "Molecular cloning and characterization of human GIPC3, a novel gene homologous to human GIPC1 and GIPC2"Int J Oncol. 20. 577-582 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T et al: "Molecular cloning and characterization of mouse Gipc3"Int J Mol Med. 20. 251-256 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T. et al.: "Molecular cloning and expression of proto-oncogene FRAT1 in human cancer"Int J Oncol. 20. 785-789 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saitoh T et al: "Expression and regulation of WNT8A and WNT8B mRNAs in human tumor cell lines: up-regulation of WNT8B mRNA by beta-estradiol in MCF-7 cells, and down-regulation of WNT8A and WNT8B mRNAs by retinoic acid in NT2 cells"Int J Oncol. 20. 999-1003 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takayama I et al.: "The interstitial cells of Cajal and a gastroenteric pacemaker system"Arch Histol Cytol. 65. 1-26 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Imai K et al.: "Zonal difference inhibition of DNA synthesis caused by anesthetics in cultured rat hepatocytes"Jap J Anesth. 51. 505-508 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagata J et al.: "Reversal of drug resistance using hammerhead ribozymes against multidrug resistance associated protein and multidrug resistance 1 gene"Int J Oncol. 21. 1021-1026 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagata J et al.: "Angiopoietin-1 and vascular endothelial growth factor expression in human esophageal cancer"Int J Mol Med. 10. 423-426 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagata J et al.: "Correlation between interleukin 10 and vascular endothelial growth factor expression in human esophageal cancer"Int J Mol Med. 10. 169-172 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saitoh T et al.: "Up-regulation of WNT8B mRNA in human gastric cancer"Int J Oncol. 20. 343-348 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saitoh T et al.: "Molecular cloning and characterization of human GIPC3, a novel gene homologous to human GIPC1 and GIPC2"Int J Oncol. 20. 577-582 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saitoh T et al.: "Molecular cloning and characterization of mouse Gipc3"Int J Mol Med. 20. 251-256 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saitoh T et al.: "Molecular cloning and expression of proto-oncogene FRAT1 in human cancer"Int J Oncol. 20. 785-789 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saitoh T et al.: "Expression and regulation of WNT8A and WNT8B mRNAs in human tumor cell lines : up-regulation of WNT8B mRNA by beta-estradiol"Int J Oncol. 20. 999-1003 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nagata J et al: "Reversal of cisplatin and multidrug resistance by ribozyme-mediated glutathione suppression"Biochem Biophys Res Commun. 286. 406-413 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saitoh T: "Proto-oncogenic WNT 10β is up-regulated by tumor necrosis factor α in human gastric cancer cell line MKN45"Inter J Oncol. 19. 1187-1192 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsukawa Y et al: "Sero prevalence of Helicobacter pylori after bone marrow transplantation"Int J Immunotherapy. 17. 21 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saitoh T: "Up-regulation of Frizzled-10(FZD10)by β-estradiol in MCF-7 cell and by retinsic acid in NT_2 cells"Inter J Oncol. 20. 117-120 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hasumi K et al: "The roles of tumor necrosis factor α receptor type 1 and Fas in the Helicobacter pylori-induced apoptosis of gastric epithelial cells"J.Gastroenterol Hepatol. (in press). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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