| Project/Area Number |
13670527
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Nagasaki University |
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Principal Investigator |
MURATA Ikuo Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学研究科, 教授 (20190910)
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| Co-Investigator(Kenkyū-buntansha) |
MURASE Kunihiko Graduate School of Biomedical Sciences, Associate professor, 大学院・医歯薬学研究科, 助教授 (30274640)
TSUKAMOTO Kazuhiro Graduate School of Biomedical Sciences, Associate professor, 大学院・医歯薬学研究科, 助教授 (30253305)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Gastroenterology / malignant tumor / malignant lymphoma / gastric MALT lymphoma / gene / genetic translocation / 胃悪性リンパ腫 / 遺伝子 / ヘリコパクターピロリ |
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| Research Abstract |
In this study, we aimed to find a new gene which may fuse to API2 gene in gastric MALT lymphoma. In the first year of the project, we examined mRNA in human biopsy specimens from 5 MALT lymphomas by using primers designed for API2 gene and putative new fused gene. However, we failed to find specific bands, partly because the primers were not specific enough to detect the putative gene.
In the second year, to get a proper primer with high specificity and the best experimental condition, we designed many primers and continued series of trials and errors on mRNA from a gastric cancer cell line-AGS cells and normal human biopsy and surgical specimens of the stomach in preliminary studies.
As a result, we could not detect a new gene that may play a role in the pathogenesis of MALT lymphoma within the project period. We intend to purify specific cDNA by nested PCR on PCR products and try to find an objective gene on the human MALT lymphoma tissue in the next year.
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