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Immunohistochemical study of disease-specific immune reaction in inflammatory bowel disease.

Research Project

Project/Area Number 13670545
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionOsaka City University Graduate School of Medicine

Principal Investigator

NAKAMURA Shiro  Osaka City University Graduate School of Medicine, 大学院・医学研究科, Instructor (50271185)

Co-Investigator(Kenkyū-buntansha) MATSUMOTO Takayuki  Osaka City University Graduate School of Medicine, 大学院・医学研究科, Assistant Professor (10209637)
Project Period (FY) 2001 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Keywordsinflammatory bowel disease / ulcerative colitis / Crohn's disease / lymphocyte / B cell / plasma cell / prolifaration / immunohistochemistry / IBD(炎症性腸疾患) / Ki67 / basal plasmacytosis / plasmacytosis / 腸間膜リンパ節 / 肉芽腫
Research Abstract

We analyzed ulcerative colitis from the standpoint of lymphocytic expansion in the loco by immunohistochem- istry and flow cytometry. Ulcerative colitis was character- ized by a diffuse distribution of Ki-67+ small round cells particularly in the ulcer base (that were CD19+ and CD20-), with a significant number of them also CD138+. Immuno- electron microscopy for CD19 revealed an abundance of rough endoplasmic reticulum in the cytoplasm. These in- dicated that they are of immature plasma lineage cells. By contrast, plasma cells in Crohn's disease were negative for CD19, and the labeling for Ki-67 was infrequent, showing mature phenotype. Flow cytometry revealed an occurrence of CD19+ and CD20- cells in ulcerative colitis but not in Crohn's disease. The labeling index of Ki-67 among CD19+ plasma cells was positively correlated with the clinical activity of ulcerative colitis. High labeling of Ki-67 in CD19+ plasma cells is specific for active ulcerative colitis that was resistant to med … More ical treatment by corticosteroids.
Next, We investigated he Th1 response and SLC and ELC in Crohn's disease pathogenesis immunohistochemicaly. ELC was almost undetectable in intestinal samples. SLC was found sporadically in lymphoid follicles, lymphoid aggregate venules, and lymphatic vessels. In mesenteric lymph nodes (MLNs), SLC was highly expressed in high endothelial venules (HEVs), lymphatic vessels, and stromal Dendritic cells (DCs), predominantly in T cell areas. ELC was highly expressed in mature DCs. There were significantly more SLC positive HEVs and ELC positive mature DCs, important components of T cell areas, in Crohn's disease. SLC, ELC, and CCR7 mRNA was significantly higher in Crohn's disease MLNs compared with Ulcerative colitis. Crohn's disease MLNs had increased expression of SLC and ELC, mainly in HEVs, mature DCs, and lymphatic vessels, inducing T cell hyperplasia. CCR7 mRNA was increased in T cell areas. The dominant Th1 immune response is facilitated by interaction of SLC positive HEVs/lymphatic vessels, ELC positive mature DCs, and CCR7 positive T cells in hyperplastic T cell areas. In Crohn's disease, memory T cells and mature DCs may home to MLN Less

Report

(5 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All 2006 2005 2004 2002 2001 2000 Other

All Journal Article (8 results) (of which Peer Reviewed: 3 results) Presentation (8 results) Publications (7 results)

  • [Journal Article] Infiltration of CD19+ plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant active ulcerative colitis.2006

    • Author(s)
      Jinno Y, et al.
    • Journal Title

      Virchows Arch. Vol 448

      Pages: 412-421

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] rifiltration of CD19+plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant activeulcerative colitis2006

    • Author(s)
      Jinno Y, et al.
    • Journal Title

      Virchows Arch. 448

      Pages: 412-421

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitis.2005

    • Author(s)
      Kawachiya T, et al
    • Journal Title

      Int J Mol Med. Vol15

      Pages: 417-423

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Augmented expression of secondary lymphoid tissue chemokine and EBI1 ligand chemokine in Crohn's disease.2005

    • Author(s)
      Kawashima D, et al.
    • Journal Title

      J Clin Pathol. Vol 58

      Pages: 1057-1063

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitis2005

    • Author(s)
      Kawachiya T, et al
    • Journal Title

      Int J Mol Med. 15

      Pages: 417-423

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Augmented expression of secondary lymphoid tissue chemokine and EBI1 ligand chemokinein Crohn's disease2005

    • Author(s)
      Kawashima D, et al.
    • Journal Title

      J Clin Pathol. 58

      Pages: 1057-1063

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Significance of increased proliferation of immature plasma cells in the appendix of patients with ulcerative colitis.2005

    • Author(s)
      Tomohiro Kawachiya, Nobuhide Oshitani, et al.
    • Journal Title

      International journal of molecular medicine 15

      Pages: 417-423

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Dislocation of tight junction proteins without F-actin disruption in inactive Crohn's disease.2005

    • Author(s)
      Nobuhide Oshitani, Kenji Watanabe et al.
    • Journal Title

      International journal of molecular medicine 15

      Pages: 407-410

    • Related Report
      2004 Annual Research Report
  • [Presentation] クローン病の病態形成におけるsecondary lymphoid-tissue chemokine(SLC)とEBI1-ligand chemokine(ELC)の役割に関する研究.2004

    • Author(s)
      川島大知, 他
    • Organizer
      第90回日本消化器病学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2004-04-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Presentation] Immunohistochemistrical analysis of SLC and ELC in Crohn's disease2004

    • Author(s)
      Daichi Kawashima
    • Organizer
      The 90th Annual meeting of the japanese society of Gastroenterology
    • Place of Presentation
      Sendai
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Presentation] 潰瘍性大腸炎病変部局所におけるCD20陰性CD19陽性細胞は形質芽細胞であり、その反応が病変を特徴づけている.2002

    • Author(s)
      神野良男, 他
    • Organizer
      第91回日本病理学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2002-03-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Presentation] Infiltration of CD19+ plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant active ulcerative colitis2002

    • Author(s)
      Yoshio Jinno
    • Organizer
      The 91th Annual meeting of the japanese society of Pathology
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Presentation] Infiltration of CD19+ plasma cells with frequent labeling of Ki-67 in corticosteroid-resistant active ulcerative colitis2002

    • Author(s)
      Yoshio Jinno
    • Organizer
      The 31th Annual meeting of the japanese society of Immunologu
    • Place of Presentation
      Osaka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Presentation] 胚中心を伴わないBリンパ球の増殖が潰瘍性大腸炎を特徴づける.2001

    • Author(s)
      神野良男, 他
    • Organizer
      第31回日本免疫学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2001-12-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Presentation] Immunohistochemistrical analysis of lymph and antigen presenting cells in Crohn's disease2001

    • Author(s)
      Shiro Nakamura
    • Organizer
      The 86th Annual meeting of the japanese society of Gastroenterology
    • Place of Presentation
      Niigata
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Presentation] クローン病におけるリンパ管と抗原提示細胞の動態に関する免疫組織化学的研究2000

    • Author(s)
      中村志郎, 他
    • Organizer
      第86回日本消化器病学会総会
    • Place of Presentation
      新潟
    • Year and Date
      2000-04-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] 松本誉之, 中村志郎, 他: "免疫異常から見たクローン病の病因・病態-腸管上皮細胞・マクロファージ・樹状細胞の役割-"G.I.Research. Vol11,no6. 27-34 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Oshitani N, Jinnno Y, Nakamura S, et al.: "Does colitid associated with Primary sclerosing cholangitis renresent an actual subset of ulcerative colitis?"Hepato-Gastroenterol. Vol50. 1830-1835 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Oshitani N. et al.: "Heterogeneity of HPLC profiles of human class II MHC-bound peptides isolated from intestine with inflammatory bowel disease"Dig Dis Sci. Vol.47. 2088-2094 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oshitani N. et al.: "Three cases of primary sclerosing cholangitis associated with ulcerative colitis; diagnostic usefulness of magnetic resonance cholangiopancreatography"Hepato Gastroenterol. 325-329 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Matsumoto T. et al.: "Role of granuloma in the immunopathogenesis of Crohn's disease"Digestion. 13-18 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Matsumoto T. et al.: "Immunopathological features of granuloma formation in the intestnial mucosa of patients of Crohn's disease"Springer-Trends in Gastreenterology and Hepatology. 370 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Oshitani N. et al.: "Decreased anti-Saccharomyces cerevisiae antibody titer by mesalazine in patients with Crohn's disease"J Gastroenterol Hepatol. 15. 1400-1403 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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