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Immuno-gene therapy for non-small lecclung cancer using innate immunity by NKG2D and its ligand.

Research Project

Project/Area Number 13670588
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionTohoku University

Principal Investigator

SAIJO Yasuo  Tohoku University Graduate School of Medicine, Department of Molecular Medicine and Gene Transfer Research, Associate Professor, 大学院・医学系研究科, 助教授 (10270828)

Co-Investigator(Kenkyū-buntansha) TOKUE Yutaka  Tohoku University School of Medicine, Research Associate, 医学部附属病院, 助手 (80292275)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordslung cancer / gene thransfer / NKG2D / RAE-1 / Fractalkine / RAE-1 / gene therapy / immunotherapy / C26
Research Abstract

NKG2D and its ligand, play a crucial role for innate immunity against to tumor. We developed adenoviral vector coding murine NKG2D ligand RAE-1 (Ad.CMV.RAE-1). When mouse colon cancer cells C26 were infected with Ad.CMV.RAE-1, these cells developed slightly slower than control C26 cells. Because RAE-1 showed very limited effects, we also constructed adenoviral vector expressing CX3C chemokine fractalkine (Ad.CMV.FKN). Intratumoral injection of Ad.CMV.FKN strongly suppressed C26 tumor by 65 % and B16 tumor by 82 %. Immunohistochemistry of tumor treated by Ad.CMV.FKN revealed abundant infiltration of NK cells, CD4 T cells, and CD8 T cells. Vaccinations with Ad.CMV.FKN treated B16 cells inhibited tumor growth of wild type B16. These results indicated that CX3C chemokine induced strong anti-tumor immune responses by recruiting NK cells and T lymphocytes.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Ishimoto O, Saijo Y, Narumi K, et al.: "High Level of Vascular Endotheial Growth Factor (VEGF) in Pleural Effusion"Oncology. 63. 70-75 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Obkouchi S, Yamada A, Saijo Y, et al.: "Non-mutated tumor-rejection antigen peptides elicit type-I allergy in the majority of healthy individuals"Tissue Antigens. 59. 259-272 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saijo Y, Tanaka M, Miki M, et al.: "Proinflammatory Cytokine Interleukin-1β Promotes Tumor Growth of Lewis Lung Carcinoma by Induction of Angiogenic Factors"Journal of Immunology. 169. 469-475 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Inoue A, Saijo Y, Maemondo M, et al.: "Severe Acute Interstitial Pneumonia and Gefitinib"Lancet. 361. 137-139 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Usui K, Saijo Y, Maemondo M, et al.: "N-terminal deletion augments the cell-death-inducing activity of BAX in the adenoviral gene delivery to nonsmall cell lung cancers"Oncogene. 22. 2655-2663 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Osamu Ishimoto, Yasuo Saijo, Ko Narumi, Yuichiro Kimura, Nobumichi Matsubara, Noboru Asou, Yushi Nakai, Masahito Ebina, Toshihiro Nukiwa.: "High level of vascular endothelial growth factor (VEGF) in pleural effusion."Oncology. 63. 70-75 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shinya Ohkouchi, Akira Yamada, Nobue Imai, Takashi Mine, Kenji Harada, Shigeki Shichijo, Yoshiaki Maeda, Yasuo Saijo, Toshihiro Nukiwa, and Kyogo Itoh.: "Non-mutated tumor-rejection antigen peptides elicit type-I allergy in the majority of healthy individuals."Tissue Antigens. 59. 259-272 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuo Saijo, Masashi Tanaka, Makoto Miki, Kazuhiro Usui, Takuji Suzuki, Makoto Maemondo, Xin Hong, Ryushi Tazawa, Toshiaki Kikuchi, Kouji Matsushima, and Toshihiro Nukiwa: "Proinflammatory cytokine interleukin-1β promotes tumor growth of Lewis lung carcinoma by induction of angiogenic factors : in vivo analysis of tumor-stromal interaction"J Immunol. 169. 469-475 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Inoue, Yasuo Saijo, Makoto Maemondo, Kazunori Gomi, Yutaka Tokue, Yuichiro Kimura, Masahito Ebina, Toshiaki Kikuchi, Takuya Moriya, and Toshihiro Nukiwa: "Severe Acute Interstitial Pneumonia and Gefitinib"Lancet. 361. 137-139 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kazuhiro Usui, Yasuo Saijo, Ko Narumi Shohei Koyama, Makoto Maemondo, Toshiaki Kikuchi, Ryushi Tazawa, Koichi Hagiwara, Yoshitomo Ishibashi, Shigeo Ohta, and Toshihiro Nukiwa.: "N-terminal deletion augments the cell-death inducing activity of BAX in adenoviral gene delivery to non-small cell lung cancers."Oncogene. 22. 2655-2663 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ishimoto O, Saijo Y, Narumi K, et al.: "High Level of Vascular Endothelial Growth Factor (VEGF) in Pleural Effusion"Oncology. 63. 70-75 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Ohkouchi S, Yamada A, Saijo Y, et al.: "Non-mutated tumor-rejection antigen peptides elicit type-I allergy in the majority of healthy individuals"Tissue Antigens. 59. 259-272 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saijo Y, Tanaka M, Miki M, et al.: "Proinflammatory Cytokine Interleukin-1β Promotes Tumor Growth of Lewis Lung Carcinoma by Induction of Angiogenic Factors"Journal of Immunology. 169. 469-475 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Inoue A, Saijo Y, Maemondo M, et al.: "Severe Acute Interstitial Pneumonia and Gefitinib"Lancet. 361. 137-139 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Iwakami S, Setoguchi Y, Saijo Y, Azuma M, Fukuchi Y.: "Replication-deficient adenovirus-mediated transfer of B7-1 (CD80) cDNA induces anti-tumor immunity in isolated human lung cancer"Respirology. 6. 135-144 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saijo Y, George S, Usui K, Sato M et al.: "Expression of nucleolar protein p120 predicts poor prognosis in patients with stage-I lung adenocarcinoma"Annals of Oncology. 12. 1121-1125 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Pradono P, Tazawa R Maemondo M, Saijo Y, et al.: "Gene transfer of thronbokisan (TX) A_2 synthase and prostaglandin (PG) I_2 synthase antithetically altered tumor angiogenesis and tumor growth"Cancer Research. 62. 63-66 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Maemondo M, Narumi K, Saijo Y, Usui K, et al.: "Inhibition of tumor angiogenesis by adenoviral mediated expression of hepatocyte growth factor antagonist, HGF/NK4"Molecular Therapy. 5. 177-185 (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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