| Project/Area Number |
13670614
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Respiratory organ internal medicine
|
|---|
| Research Institution | DOKKYO UNIVERSITY SCHOOL OF MEDICINE |
|---|
Principal Investigator |
ISHII Yoshiki DOKKYO UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PULMONARY MEDICINE AND CLINICAL IMMUNOLOGY, ASSOCIATE PROFESSOR, 医学部, 助教授 (20254914)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Fumiya DOKKYO UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PULMONARY MEDICINE AND CLINICAL IMMUNOLOGY, 医学部, 助手 (50333000)
MIYOSHI Masaaki DOKKYO UNIVERSITY SCHOOL OF MEDICINE, DEPARTMENT OF PULMONARY MEDICINE AND CLINICAL IMMUNOLOGY, 医学部, 助手 (90326900)
|
|---|
| Project Period (FY) |
2001 – 2002
|
| Project Status |
Completed (Fiscal Year 2002)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2001: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | pulmonary fibrosis / dendritic cell / fibroblast / collagen / peripheral blood stem cell / antigen presenting cell / 抗原提示能 / 血液幹細胞 / 筋線維芽細胞 / 線維芽細胞様樹状細胞 |
|---|
| Research Abstract |
We isolated fibroblast-like dendritic cells (FDC) from human peripheral blood and examined their characteristics. Although adherent FDC showed fibroblast-like appearance on a culture plate, they demonstrated dendritic cell-like villi on the cell surface when removed from the plate. FDC can be differentiated from not only CD34 positive progenitor cells but also CD14 positive-monocytes. We found that FDC expressed higher levels of type I collagen and fibronectin mRNA than monocyte-derived DC from the same individuals or alveolar macrophages. FDC also displayed cell surface antigens such as CD83, CD86, and HLA-DR. FDC induced antigen-presenting cell-dependent naive T cell proliferation. This proliferation acitivity was significantly higher than that induced by monocytes. Furthermore, we demonstrated that TGF-β1 (10ng/ml), an important fibrogenic cytokine, increased the differentiation and expression of α-smooth muscle actin and prolyl 4-hydroxylase. These findings indicated that FDC have both fibroblast-like and DC-like characteristics. To clarify the role of FDC in the pathophysiology of pulmonary fibrosis might be very useful to develop a new therapy.
|
