| Project/Area Number |
13670691
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAJIMA Toshiaki The University of Tokyo, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (50227790)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Smooth muscle / Ca^<2+> permeable channel / Transient receptor potential / endothelin-1 / Ca^<2+>-ion / Non-selective cation channel / smooth muscle / Patch-clany / nonselective cation channel / Transient receptor potential / Endothelin-1 / Intracellular Ca^<2+> concentration |
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| Research Abstract |
The contractile agonists such as endothelin-1 and vasopressin increase Ca^<2+> influx via several receptor-activated Ca^<2+> channels (Ca^<2+>-release-activated Ca^<2+> channel (CRAC) and Ca^<2+> permeable nonselective cation channels (I_<CAT>)). The analysis of RT-PCR showed the expression of transient receptor potential (TRP1,2,4) and TRP6. The latter may be related to I_<CAT>, and the former may be related to CRAC. Diacylglycerol produced from the activation of PLC, but not IP_3 and protein kinase C, may be involved in the activation of I_<CAT>. Similar results were obtained in human bronchial smooth muscle cells (Am J Respir Cell Mol Biol, 2000 ; 2002). In addition, we have reported that I_<CAT> plays an important role in forming membrane potential in rabbit coronary arterial cells, and lysophosphatidylcholine (LPC) further activated I_<CAT>. Thus, it is likely that I_<CAT> may play a role in regulating smooth muscle tone under the pathophysiological conditions.
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