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Development of a Gene Transfer and Functional Evaluation System for an Isolated Single Cardiac Myocyte as a model for the Gene Therapy

Research Project

Project/Area Number 13670692
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionThe University of Tokyo

Principal Investigator

SUGIURA Seiryo  The University of Tokyo, Graduate School of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (10272551)

Co-Investigator(Kenkyū-buntansha) SATA Masataka  The University of Tokyo, Graduate School of Medicine, Assistant Professor, 医学部附属病院, 助手 (80345214)
YAMASHITA Hiroshi  The University of Tokyo, Graduate School of Medicine, Assistant Professor, 医学部附属病院, 助手 (50323572)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordscardiac myocyte / contractile force / carbon fiber
Research Abstract

In order to study the mechanical activity of a single cardiac myocyte under a wide range of load, we have developed a novel force measurement system using carbon fibers. Newly fabricated Carbon fibers greatly facilitate the firm attachment of cell membrane to the fibers. A pair of fibers was attached to both ends of the cell ; the rigid fiber as a mechanical ground and the compliant fiber for the strain gauge. By connecting the compliant fiber to the piezoelectric translator and applying the position signal to the driver, we could make the myocyte contract under wide range of loading conditions. Furthermore, we have implemented the optical system for the measurement of intracellular calcium concentration thus the simultaneous recording of force and calcium transient of a single cardiac myocyte was made possible. We observed a strong load dependence of calcium transient, which shows clear contrast to the previous reports. Feedback control of the system also enabled us to study the function of a single cardiac myocyte under physiological loading sequence. We have also measured the force developed by a myocyte transfected with GFP adenovirus. This system can be a useful tool in studying the mechanical activity of the cardiac myocyte under genetic interventions thus serving as a useful tool in the study of heart failure.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Yasuda S, Sugiura S, Kobayakawa N, Fujita H, Yamashita H, Katoh K, Saeki Y, Kaneko H, Suda Y, Nagai R, Sugi H: "A novel method to study contraction characteristics of a single cardiac myocyte using carbon fibers coupled with a feedback system"Am J Physiol. 281. H144-H1446 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saeki Y, Takigiku K, Iwamoto H, Yasuda S, Yamashita H, Sugiura S, Sugi H: "Protein Kinase A increases the rate of relaxation but not the rate of tension development in skinned rat cardiac muscle"Jpn J Physiol. 21. 427-433 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sata M, Nishimatsu Suzuki E, Sugiura S, Yoshizumi M, Ouchi Y, Hirata Y, Nagai R: "Endothelial nitric oxide synthase is essential for the HMG-GoA reductase inhibitor cerivastatin to promote collateral growth in response to ischemia"FASEB J. 15. U20-U33 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] WATANABE H, HISADA T, SUGIURA S, OKADA J, FUKUNARI H: "Computer Simulation of Blood Flow, Left Ventricular Wall Motion and Their Interrelationship by Fluid-Structure Interaction Finite Element Method"JSME International Journal Series C. 45. 1003-1012 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuda S, Sugiura S, Kobayakawa N, Fujita H, Yamashita H, Katoh K, Saeki Y, Kaneko H, Suda Y, Nagai R, and Sugi H: "A novel method to study contraction characteristics of a single cardiac myocyte using carbon fibers coupled with a feedback system"Am J Physiol. 281. H1442-1446 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Saeki Y, Takigiku K, Iwamoto H, Yasuda S, Yamashita H, Sugiura S and Sugi H: "Protein kinase A increases the rate of relaxation but not the rate of tension development in skinned rat cardiac muscle"Jpn J Physiol. 51. 427-433 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sata M, Nishimatsu H, Suzuki E, Sugiura S, Yoshizumi M, Ouchi Y, Hirata Y, Nagai R, Masataka Sata, Hiroaki Nishimatsu, Etsu Suzuki, Seiryo Sugiura, Masao Yoshizumi, Yasuyoshi Ouchi, Yasunobu Hirata, Ryozo Nagai: "Endothelial nitric oxide synthase is essential for the HMG-CoA reductase inhibitor cerivastatin to promote collateral growth in response to ischemia"FASEBJ. 15. U20-U33 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] WATANABE H, HISADA T, SUGIURA S, OKADA J, and FUKUNARI H: "Computer Simulation of Blood Flow, Left Ventricular Wall Motion and Their Interrelationship by Fluid-Structure Interaction Finite Element Method"JSME International Journal Series C. 45. 1003-1012 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasuda S, Sugiura S, Kobayakawa N, Fujita H, Yamashita H, Katoh K, Saeki Y, Kaneko H, Suda Y, Nagai R, Sugi H: "A novel method to study contraction characteristics of a single cardiac myocyte using carbon fibers coupled with a feedback system"Am J Physiol. 281. H144-H1446 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Saeki Y, Takigiku K, Iwamoto H, Yasuda S, Yamashita H, Sugiura S, Sugi H: "Protein Kinase A increases the rate of relaxation but not the rate of tension development in skinned rat cardiac muscle"Jpn J Physiol. 21. 427-433 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sata M, Nishimatsu H, Suzuki E, Sugiura S, Yoshizumi M, Ouchi Y, Hirata Y, Nagai R: "Endothelial nitric oxide synthase is essential for the HMG-CoA reductase inhibitor cerivastatin to promote collateral growth in response to ischemia"FASEB J. 15. U20-U30 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] WATANABE H, HISADA T, SUGIURA S, OKADA J, FUKUNARI H: "Computer Simulation of Blood Flow, Left Ventricular Wall Motion and Their Interrelationship by Fluid-Structure Interaction Finite Element Method"JSME International Journal Series C,. 45. 1003-1012 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yasuda S: "Anovel method to study contraction characteristics of a single cardiac myocyte using carbon fibers coupled with a feedback system"Am J Physiol. 281. H1442-H1446 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Saeki Y: "Protein kinase A increases the rate of relaxation but not the rate of tension development in skinned rat cardiac muscle"Jpn J Physiol. 51. 427-433 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sata M: "Acute and chronic smooth muscle cell apoptosis following mechanical vascular injury can occur independently ofthe Fas-death pathway"Arteriosclerosis, Thrombosis, and Vascular Biology. 21. 1733-1737 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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