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Modulation of ventricular spiral-wave reentry by cardiac ion channel blockade

Research Project

Project/Area Number 13670702
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionResearch Institute of Environmental Medicine, Nagoya University

Principal Investigator

HONJO Haruo  Nagoya University, Research Institute of Environmental Medicine, Associate Professor, 環境医学研究所, 助教授 (70262912)

Co-Investigator(Kenkyū-buntansha) SAKUMA Ichiro  The University of Tokyo, Graduate School of Frontier Sciences, Professor, 大学院・新領域創成科学研究科, 教授 (50178597)
KODAMA Itsuo  Nagoya University, Research Institute of Environmental Medicine, Professor, 環境医学研究所, 教授 (30124720)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsspiral reentry / optical mapping / ventricular tachycardia / antiarrhythmic drug / anisotropic conduction / curvature effect / excitable gap / 活動電位 / 坑不整脈薬 / スパイラルリエントリー / ブロックライン
Research Abstract

It is widely accepted that functional reentry plays an important role in the initiation and maintenance of ventricular tachycardia/fibrillation (VT/VF). Various concepts including "leading circle", anisotropic reentry and spiral-type excitation have been proposed to explain the functional reentry in the heart but its precise mechanisms remain to be elucidated. We investigated dynamics and pharmacological modulation cf VT by high-resolution optical mapping. Two-dimensional subepicardial ventricular myocardial sheet (〜1 mm in thickness) of the Langendorff-perfused rabbit heart was made by cryoablation of the endocardial side of the left ventricle. The heart was stained with di-4-ANEPPS (2 μM), and fluorescence images of theventricular myocardium were obtained via a high-speed video camera (temporal and spatial resolutions of〜1.3 ms and 〜0.1 mm, respectively). VT induced by cross-field stimulation was often the result of single reentry around a "Z"-shaped functional line of block. The cen … More tral segment of the block line, which was independent of the myocardial fiber direction, was associated with an abrupt phase-shift of the action potential. Small amplitude double potentials without electrical diastole were obtained at the central block line suggesting "leading circle"-type electrotonic interaction. The two terminal segments were caused by localized conduction delay around pivot points of the rotation and these lines were always parallel to the myocardial fiber direction. Source-to-sink imbalance created by wavefront curvature combined with tissue anisotropy may be responsible for the localized conduction delay around the pivot paints. Blockade of Na^+ channels by pilsicainide, disopyramide and cibenzoline extended the terminal block lines through the enhancement of the localized local conduction delay and caused appreciable electrical diastole (excitable gap) in the circuit except around pivot points. These results suggest that functional reentry in the two-dimensional ventricular myocardium is determined by wavefront-tail interaction and source-sink imbalance caused by wavefront curvature and tissue anisotropy. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Mitsuru Yamamoto et al.: "Sustained monomorphic ventricular tachycardia in the two-dimensional left ventricular epicardial sheets of rabbit hearts perfused in-vitro"Environmental Medicine. 45. 92-94 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Motoki Nihei et al.: "High-resolution optical mapping system with high-speed digital video camera"Environmental Medicine. 45. 103-105 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Motoki Nihei et al.: "Na^+ channel bockade causes a prolongation of electrical diastole during spiral-type reentry in the ventricle"Environmental Medicine. 46. 103-105 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 本荘 晴朗 他: "心室スパイラルエントリーの成立機構と薬物作用"心臓. 33. 449-455 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 佐久間 一郎 他: "高輝度発光ダイオードと高速度ビデオカメラを用いる心臓膜電位マッピングシステム"心臓. 33. 439-448 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 児玉 逸雄 他: "心室のスパイラル・リエントリー"心臓. 34. 918-925 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 柴田 仁太郎 他: "心室筋レベルでの心室細動と電気的除細動"ICUとCCU. 26. 233-240 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 柴田 仁太郎 他: "心室細動と除細動のメカニズム"ICUとCCU. 26. 661-665 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Akira Mishima et al.: "A high-resolution optical mapping and analysis system of action potentials during ventricular arrhythmias"Proceedings of the Third China-Japan-Korea Joint Symposium on Medical Informatics. 53-55 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Mitsuru Yamamoto et al.: "Sustained monomorphic ventricular tachycardia in the two-dimensional left ventricular epicardial sheets of rabbit hearts perfused in-vitro"Environmental Medicine. 45. 92-94 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Motoki Nihei et al.: "High-resolution optical mapping system with high-speed digital video camera"Environmental Medicine. 45. 103-105 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Motoki Nihei et al.: "Na+ channel blockade causes a prolongation of electrical diastole during spiral-type reentry in the ventricle"Environmental Medicine. 46 in press. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Motoki Nihei et al.: "Na^+ channel blockade causes a prolongation of electrical diastole during spiral-type reentry in the ventricle"Environmental Medicine. 46. 103-106 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 児玉 逸雄 他: "心室のスパイラル・リエントリー"心臓. 34. 918-925 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 山本 充 他: "心室スパイラル・リエントリーに対するI群坑不整脈薬の作用"Progress in Medicine. 22. 2563-2570 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 柴田 仁太郎 他: "心室細動と除細動のメカニズム"ICUとCCU. 26. 601-665 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 柴田 仁太郎 他: "心室細動と除細動のメカニズム"ICUとCCU. 26. 233-240 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Mitsuru Yamamoto et al.: "Sustained monomorphic ventricular tachycardia in the two-dimensional left ventricular epicardial sheets of rabbit hearts perfused in vitro"Environmental Medicine. 45. 92-94 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Motoki Nihei et al.: "High-resolution optical mapping system with high-speed digital video camera"Environmental Medicine. 45. 103-105 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 佐久間一郎 他: "高輝度発光ダイオードと高速度ビデオカメラを用いる心臓膜電位マッピングシステム"心臓. 33. 439-448 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 本荘晴朗 他: "心室スパイラルリエントリーの成立機構と薬物制御"心臓. 33. 449-455 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Haruo Honjo et al.: "Blockade of I_<Kr> promotes termination of spiral-type reentrant arrhythmia in the ventricle"Japanese Circulation Journal. 65 (Suppl.I-A). 459 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mitsuru Yamamoto et al.: "Effects of disoryramide on spiral dynamics during ventricular tachycardia"Cardiovascular Drugs and Therapy. 15 (Suppl.1). 45 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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