| Project/Area Number |
13670792
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nagoya University |
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Principal Investigator |
KOJIMA Seiji Nagoya University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (20313992)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Hiroshi Nagoya University, University Hospital, Assistant Professor, 医学部附属病院, 講師 (30303621)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2002: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Hepatitis / Aplastic Anemia / Apoptosis / Fas antigen / Hepatitis E / HLA / E型肝炎ウィルス / パルボB19ウィルス |
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| Research Abstract |
Hepatitis-associated aplastic anemia (H-AA) is a varient of acquired AA, in which hepatitis precedes AA by a period of weeks to months. Both etiology of hepatitis and a mechanism of bone marrow failure are unknown. Although hepatitis E virus (HEV) was studied by HEVIgG and HEVIgM in 16 patients with H-AA, it was not detected in all of them. Based on our result, it is concluded that HEV is not a candidate virus for H-AA. We studied HLA antigen in 30pts. with H-AA, 60pts. with idiopathic AA and 250 normal controls. The frequencies of DR9 antigen was significantly increased in the pts. with H-AA, compared to pts, with idiopathic AA or normal controls (relative risk : 4.1). In pts with HAA, absolute numbers of total lymphocytes, especially CD4 lymphocytes are markedly decreased. We analyzed the expression of Fas antigen on peripheral lymphocytes and BM CD34+cells of pts. with AA. Higher expression of Fas antigen on peripheral lymphocytes and BM CD34+cells was found in pts. with H-AA than in pts. with idiopathic AA or normal controls. Expression of Fas-ligand was also increased on peripheral and BM CD+3 lymphocytes. These results suggest that increased programmed cell death though Fas/Fas ligand system in HLA restricted individuals is a possible mechanism of bone marrow failure in pts. with H-AA.
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