Indirect immunofluorescence using anti MLL gene protein
| Project/Area Number |
13670809
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
UEDA Kazuhiro Hiroshima University Graduate School of Biomedical Sciences Professor, 大学院・医歯薬学総合研究科, 教授 (30112189)
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| Co-Investigator(Kenkyū-buntansha) |
TASHIRO Satoshi Hiroshima University Graduate School of Biomedical Sciences Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (20243610)
NISHIMURA Shin-ichiro Hiroshima University Medical Hospital Assistant Professor, 医学部附属病院, 講師 (00228222)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | MLL gene / indirect immunofluorescence / infant leukemia |
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| Research Abstract |
First, we prepared poly-clonal antibody to MLL gene protein, but this poly-clonal antibody was not specific for MLL gene protein by Western blotting analysis. So, other anti MLL gene protein antibody (provided by Dr.Seto, Aichi Cancer Institute) was used for further analysis. By immunofluorescence using this antibody, MLL gene and MLL chimeric gene was located at nucleus of leukemic cells. However, we could not detect the difference of distribution between MLL *ne and MLL chimeric gene protein on the nucleus of the leukemic cell. Quantitative analysis of the protein was also difficult by immunohistochemistry using this antibody. New mono-clonal antibody, which detect the difference between MLL gene and MLL chimeric gene protein, should be synthesized.
Although we analyzed few clinical samples, rapid diagnosis of infant leukemia by immunofluorescence using anti MLL gene protein antibody could not be established. For diagnosis of infant leukemia with chromosomal translocations, multiplex PCR analysis may be superior than immunohistochemistry. In the clinical study of childhood acute lymphoblastic leukemia of JACLS : Japan Association of Childhood Leukemia Study, multiplex PCR analysis will be underwent at all patients with denove acute lymphoblastic leukemia.
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Report
(3 results)
Research Products
(17 results)
