| Project/Area Number |
13670825
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Nagoya City University |
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Principal Investigator |
BAN Kyoko (2002) Nagoya City University, Graduate School of Medical Sciences Research Associate., 大学院・医学研究科, 助手 (90285213)
杉山 幸八郎 (2001) 名古屋市立大学, 医学部, 講師 (60117827)
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| Co-Investigator(Kenkyū-buntansha) |
坂 京子 名古屋市立大学, 医学部, 助手 (90285213)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | TT Virus / SEN Virus-D / SEN Virus-H / Infection Route / Hepatitis / Mother-to-Infant Transmission / SENV |
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| Research Abstract |
The pathogenicity of TT virus in childhood was investigated.
1. Aassessment of genotype in the non-A to C hepatic dysfunction group revealed a higher prevalence of genotype 1 than of all other genotypes. This result differed significantly from that of the control group. Overrepresentation of genotype 1 suggests that this type of TTV strain is associated with the development of hepatic dysfunction of unknown etiology in Japanese children.
2. The rates for detection of serum TTV in 12 serum HCV-positive mothers and their infants were tested. Serum TTV DNA was not detected in any infant at 1 month of age, but was detected for the first time between 1.5 and 8 months after birth. Positivity persisted thereafter throughout the follow-up period. In seven randomly selected mother-infant pairs, intrahost TTV heterogeneity was lower in infants than in mothers. One of seven mother-infant pairs showed a high degree of similarity (98.7-100%) in all clones, while in four infants, all nucleotide sequences differed by >10% from those of their mothers. However, the degree of homology in the two mother-infant pairs was 89-98.7% in family 2 and 88.1-99.4% in family 5. In the present study, with only one exception, it was shown that TTV from infants is not identical to TTV from mothers.
3. To clarify the pathogenesis of SEN virus in childhood, we investigated the detection rates of serum SEN virus type D and H among 100 Japanese children with or without liver disease. Children with liver disease were divided into fulminant hepatitis, acute hepatitis, and chronic hepatitis group. No significant difference was found among each group, except between control group and fulminant hepatitis group for SEN virus-D.
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