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DEVELOPMENT OF THE NEW TECHNOLOGY FOR TISSUE ENGINEERING OF CULTURED SKIN USING DEVELOPMENTAL AND REGENERATIVE MOLECULAR SIGNALING.

Research Project

Project/Area Number 13670864
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionAKITA UNIVERSITY

Principal Investigator

TAKASHI Kameda  AKITA UNIVERSITY, School of Medicine, RESEARCH ASSOCIATE, 医学部, 助手 (00301119)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsShh / cultured epithelium / feeder cell / stem cell / hair regeneration / cell linage / 幹細胞 / 再生医工学 / 培養表皮 / Wnt-3 / 分子発生学
Research Abstract

In this project, we tried to improve the tissue engineering technology for production of cultured skin using developmental and regenerative molecular mechanism. We developed a new technology to accelerate the formation of the cultured epithelium by using Sonic hedgehog expressing feeder cells. This result indicates that the application of the knowledge from the molecular biology is useful to develop the tissue engineering (T. Kameda, Tissue Eng, 2001). We also analyzed the nature of epidermal stem cells in hair bulge or basal layer of epidermis. Elimination of the label-retaining cells in the bulge by photo-induced cell killing method, which we developed, caused a defect on hair renewal (T. Kameda, Genes Cells, 2002). This result support the idea that the label-retaining cells in the bulge is the hair stem cell. And, we analyzed the distribution pattern of the epidermal stem cells in basal layer of mouse ear epidermis by an in vitro and in vivo analysis using retrovirus vector, and proposed new hypothesis for the epidermal renewal unit (T. Kameda, Exp Cell Res, 2003).

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] 亀田 隆: "Analysis of the cellular heterogeneity in the basal layer of mouse ear epidermis: an approach from partial decomposition in vitro and retroviral cell marking in vivo"Exp Cell Res. 283. 167-183 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 亀田 隆: "Targeted elimination of the follicular label-retaining cells by photo-induced cell killing caused a defect on follicular renewal on mice"Genes Cells. 7. 923-931 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 永井宏和: "Differentiation of liver epithelial (stem like) cells into hepatocytes induced by coculture with hepatic stellate cells"Biochem Biophys Res Commun. 293. 1420-1425 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 小池千加: "Introduction of wild-type patched gene suppresses the oncogenic potential of human squamous cell carcinoma cell lines including A431"Oncogene. 21. 2670-2678 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 亀田 隆: "Acceleration of the formation of cultured epithelium using Sonic hedgehog expressing feeder cells"Tissue Eng. 7. 545-555 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 筏義人: "再生医療工学の最先端"シーエムシー出版. 265 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kameda: "Analysis of the cellular heterogeneity in the basal layer of mouse ear epidermis: an approach from partial decomposition in vitro and retroviral cell marking in vivo."Exp Cell Res. 283. 167-183 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kameda: "Targeted elimination of the follicular label-retaining cells by photo-induced cell killing caused a defect on follicular renewal on mice."Genes Cells. 7. 923-931 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Nagai: "Differentiation of liver epithelial (stem like) cells into hepatocytes induced by coculture with hepatic stellate cells."Biochem Biophys Res Commun. 293. 1420-1425 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] C.Koike: "Introduction of wild-type patched gene suppresses the oncogenic potential of human squamous cell carcinoma cell lines including A431."Oncogene. 21. 2670-2678 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Kameda: "Acceleration of the formation of cultured epithelium using Sonic hedgehog expressing feeder cells."Tissue Eng. 7. 545-555 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.IKADA: "The newest point of regenerative medicine"CMC Press. 265 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 亀田 隆: "Analysis of cellular heterogeneity in the basal layer of mouse ear epidermis: an approach from partial decomposition in vitro and retroviral cell marking in vivo"Exp.Cell Res.. 283・2. 167-183 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 亀田 隆: "Targeted elimination of the follicular label-retaining cells by photo-induced cell killing caused a defect on follicular renewal on mice"Genes.Cells. 7・9. 923-931 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 永井宏和: "Differentiation of liver epithelial (stem like) cells into hepatocytes induced by coculture with hepatic stellate cells"Biochem.Biophys.Res.Commun.. 293・5. 1420-1425 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 小池千加: "Introduction of wild-type patched gene suppresses the oncogenic potential of human squamous cell carcinoma cell lines including A431"Oncogene. 21・17. 2670-2678 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 筏 義人: "再生医工学の最先端"シーエムケー出版. 265 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 亀田 隆: "Acceleration of the formation of cultured epithelium using the Sonic hedgehog expressing feeder cells"Tissue Engineering. 7・5. 545-555 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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