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Anakysis of GATA-3 function in proliferation and differentiation of kerationocytes

Research Project

Project/Area Number 13670865
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionUniversity of Tsukuba

Principal Investigator

KAWACHI Yasuhiro  Inst. of Clinical Medicine, Assistant Professor, 臨床医学系, 講師 (00272196)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsGATA-3 / Keratinocytes / 表皮 / ロリクリン / keratinocytes
Research Abstract

In epidermal keratinocytes, we demonstrated that GATA-3 was more abundant in differentiated keratinocytes than undifferentiated keratinocytes, suggesting that GATA-3 might contribute to differntiatioirspecific gene regulation. We previously showed that c-Fos and Sp1 activate the loricrin promoter activity in differentiated keratinocytes while c-Jun and Sp3 suppress it in undifferentiated keratinocytes. Thus, we tested the cooperative tarnsactivation of loricrin promoter activity by GATA-3, c-Fos and Sp1. Transactivation experiments presented here provide evidence that GATA-3, c-Fos and Sp1 can activate the loricrin promoter transcription in a synergistic manner even though the GATA-3 binding motif is deleted from the promoter. These results strongly suggest the interaction among those proteins. Thus, we examined the physical binding among GATA-3, AP-1 and Sp1/Sp3. In vitro binding by means of a glutathione S-transferase pull down assay showed that c-Jun/c-Fos could directly associate with GATA-3. However, binding affinity of GATA-3 with Sp1 was much less than that with c-Fos, These results suggest that GATA-3 can interact with c-Fos directly while GATA-3 may interact with Sp1 via other transcription factors or cofactors.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Waikel RL, Kawachi Y et al.: "Deregulation expression of c-Myc depletes epidermal stem cells"Nature Genetics. 28. 165-168 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ichikawa E, Kawachi Y et al.: "Hereditary complement(C9) deficiency associated with dermatomyositis"British Journal of Dermatology. 144. 1080-1083 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koch PJ, Kawachi Y et al.: "Lessons from loricrin-deficient mouse : compensatory mechanisms maintaining skin barrier function in the absence of a major cornified envelope protein"Journal of Cell Biology. 151. 389-400 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kotsuji T, Kawachi Y et al.: "PDGF-BB induces MAP kinase phosphorylation and VEGF expression in neurofibroma-derived cultured cells from patients with NF-1"Journal of Dermatology. 29. 713-717 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawachi Y et al.: "Expression of angiogenic factors in neurofibromas"Experimental Dermatology. 11. 1-6 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Koch PJ, de Viragh PA, Scharer E, Bundman D, Longley MA, Bickenbach J, Kawachi Y, Suga Y, Zhou Z, Huber M, Hohl D, Kartasova T, Jarnik M, Steven AC, Roop DR.: "Lessons from loricrin-deficient mice : compensatory mechanisms maintaining skin barrier function in the absence of a major cornified envelope protein"Journal of Cell Biology. 151. 389-400 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Fujisawa H, Ishii Y, Tateishi T, Kawachi Y, Otsuka F, Amagai M, Komai A, Hashimoto T.: "Pemphigoid nodularis with IgA autoantibodies against the intracellular domain of desmoglein 1"British Journal of Dermatology. 141. 143-147 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Waikel RL, Kawachi Y, Waikel PA, Wang XJ, Roop DR.: "Deregulated expression of c-Myc depletes epidermal stem cells"Nature Genetics. 28. 165-168 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Ichikawa E, Furuta J, Kawachi Y, Imakado S, Otsuka F.: "Hereditary complement 9 deficiency associated with dermatomyositis"British Journal of Dermatology. 144. 1080-1083 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kotsuji-Maruyama T, Imakado S, Kawachi Y, Otsuka F.: "PDGF-BB induces MAP kinase phosphorylation and VEGF expression in neurofibroma-derived cultured cells from patients with neurofibromatosis-1"Journal of Dermatology. 29. 713-717 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawachi Y, Xu X, Ichikawa E, Imakado S, Otsuka F: "Expression of angiogenic factors in neurofibromas"Experimental Dermatology. 11. 1-6 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kotsuji T.Kawachi Y et al.: "PDGF-BB induces MAP kinase phosphorylation and VEGF expression in neurofibroma-derived cultured cells from patients with NF-1"Journal of Dermatology. 29. 713-717 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kawachi Y et al.: "Expression of angiogenic factors in neurofibromas"Experiental Dermatology. 11. 1-6 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Waikel RL., Kawachi Y. et al.: "Deregulated expression of c-Myc depletes epidermal sterm cells"Nature Genetics. 28(2). 165-168 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ichikawa E., Kawachi Y. et al.: "Hereditary complement (C9) deficiency associated with dermatomyositis"British Journal of Dermatology. 144(5). 1080-1083 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Koch PJ., Kawachi Y. et al.: "Lessons from loricrin-deficient mouse : compensatory mechanisms maintaining skin barrier fanction in the absence of a major cornified envelope protein"Journal of Cell Biology. 151(2). 389-400 (2000)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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