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THE ANALYSIS OF CHROMOSOMAL GAINS AND LOSSES IN PRIMARY MALIGNANTMELANOMA-COMPARISON WITH SPITZ NEVUS-

Research Project

Project/Area Number 13670875
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionSHINSHU UNIVERSITY

Principal Investigator

MATSUMOTO Kazuhiko  SHINSHU UNIVERSITY HOSPITAL, ASSISTANT PROFESSOR, 医学部附属病院, 講師 (40165882)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2002: ¥400,000 (Direct Cost: ¥400,000)
KeywordsMELANOMA / SPITZ NEVUS / MICRODISSECIION / COMPARATIVE GENOMIC HYBRIDIZATION
Research Abstract

SPITZ NEVUS IS A BENIGN NEOPLASM OF MELANOCYTES THAT CAN BE DIFFICULT OR IMPOSSIBLE TO DISTINGUISH FROM MELANOMA BY CLINICAL AND HISTOPATHOLOGIC EXAMINATION. WE TRIED TO FIND CHROMOSOMAL GAIN AND LOSSES BY COMPARATIVE GENOMIC HYBRIDIZATION (CGH) IN FORMALIN-FIXED, PARAFFIN ENMBEDDED TISSUES FROM MELANOMA, AND COMPARE WITH THAT FROM SPITZ NEVUS. HOWEVER, THE SIZE OF DNA EXTRACTED FROM FORMARIN-FIXED, PARAFFIN-EMBEDDED TISSUE IS TOO SMALL FOR CGH. THEREFORE, WE FIRST TRIED TO ESTABLISH THE CGH METHOD USING MELANOMA CELL LINE, MMG1. REFERENCE DNA WAS EXTRATED FROM NORMAL FEMALE PERIPHERAL BLOOD LYMPHOCYTES. WE GOT THE FOLLOWING RESULTS.
1. EXTRACTION OF DNA : DNA EXTRACTOR WB KIT (WAKO, OSAKA) CAN BE USED FOR THE EXTRACTION FROM THE CELL LINES.
2. LABELING TIME : ONE AND A HALF HOUR FOR MMG1 CELLS. THREE HOURS FOR PERIPHERAL BLOOD LYMPHOCYTES.
3. PRECIPITATION TIME WITH ETHANOL : -80℃, ONE HOUR
4. HYBRIDIZATION TIME: 37℃, 3 DAYS
WE HAVE ESTABLISHED THE CGH METHOD IN OUR LABORATORY THE FINAL PROBLEM IS THE DNA EXTRACTION FROM FORMARIN-FIXED, PARAFFIN-EMBEDDED TISSUES. WE WILL SOLVE THE PROBLEM SOON AND WE CAN FIND CHROMOSOMAL GAIN AND LOSSES BY COMPARATIVE GENOMIC HYBRIDIZATION (CGH) IN FORMALIN-FIXED, PARAFFIN ENMBEDDED TISSUES FROM MELANOMA AND SPITZ NEVUS.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Kageshita T: "Growth inhibition of human malignant melanoma transfected with the human interferon-β gene by means of cationic liposomes"Melanoma Research. 11. 337-342 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kageshita T: "Loss of β-catenin expression associated with disease progression in malignant melanoma"British Journal of Dermatology. 145. 210-216 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Oguchi M: "Expression of lamins depends on epidermal differentiation and transformation"British Journal of Dermatology. 147. 853-858 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 松本和彦: "左拇趾球部に生じた小型の悪性黒色腫"Visual Dermatology. 1. 40-41 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 福沢正男: "白斑を合併し、原発巣の部分退縮を示した悪性黒色腫の1例"臨床皮膚科. 56. 245-248 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] KAGESHITA T: "GROWTH INHIBITION OF HUMAN MALIGNANT MELANOMA TRANSFECIED WITH THE HUMAN INTERFERON-β GENE BY MEANS OF CATIONIC LIPOSOMES"MELANOMA RESEARCH. 11. 337-342 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] KAGESHITA T: "LOSS OF β-CATHENIN EXPRESSION ASSOCIATED WITH DISEASE PROGRESSION IN MALIGNANT MELANOMA"BRITISH JOURNAL OF DERMATOLOGY. 145. 210-216 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] OGUCHI M: "EXPRESSION OF LAMINS DEPENDS ON EPIDERMAL DIFFERENTIATION AND TRANSFORMATION"BRITISH JOURNAL OF DERMATOLOGY. 147. 853-858 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] MATSUMOTO K: "SMALL MALIGNANT MELANOMA ON THE BALL OF THE LEFT FOOT"VISUAL DERMATOLOGY. 1. 40-41 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] FUKUZAWA M: "A CASE OF MALIGNANT MELANOMA WITH PARTIAL RFGRESSION OF PRIMARY LESION AND VITILIGO"RINNSHO HIFUKA. 56. 245-248 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kageshita T: "Growth inhibition of human malignant melanoma transfected with the human interferon-β gene by means of cationic liposomes"Melanoma Research. 11. 337-342 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kageshita T: "Loss of β-catenin expression associated with disease progression in malignant melanoma"British Journal of Dermatology. 145. 210-216 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Oguchi M: "Expression of lamins depends on epidermal differentiation and transformation"British Journal of Dermatology. 147. 853-858 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 松本和彦: "左拇趾球部に生じた小型の悪性黒色腫"Visual Dermatology. 1. 40-41 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] 福沢正男: "白斑を合併し、原発巣の部分退縮を示した悪性黒色腫の1例"臨床皮膚科. 56. 245-248 (2002)

    • Related Report
      2002 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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