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Analysis of ionizing radiation effects on mitochondrial metabolisms of human cancer cells.

Research Project

Project/Area Number 13670961
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionDokkyo Medical University

Principal Investigator

FURUTA Masaya  Dokkyo University, School of Medicine, Vice-professor, 医学部, 助教授 (00261850)

Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2002: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2001: ¥900,000 (Direct Cost: ¥900,000)
Keywordsmitochondoria / ionizing radiation / tumor metabolism / sublethal damage / hyperthermia / 放射線治療 / 肺がん
Research Abstract

The changes in mitochondrial metabolisms of human cancer cells following x-ray irradiation were studied. The metabolisms were examined by (1) measuring cellular uptake of Tc-99m hexakis-2-methoxy-isobutyl-isonitrile (MIBI) and (2) measuring the mitochondrial membrane potentials by flow cytometry with a cyanine dye, 3,3'-dihexyloxacarbocyanine iodide (DiOC6(3)). A human lung cancer cell line, A549 was used for the study.
1.Tc-99m MIBI accumulation in the cancer cells increased at 1 hr following 3 Gy and 9 Gy irradiation by 10.5 +/-1.6% and 16.8 +/-5.6%, respectively, compared to control (p<0.01). Thereafter, once the cellular uptake returned to the control level, and increased again at 8 hr after 9 Gy irradiation by 14.6 +/-3.6% (p<0.05). This second peak of uptake was not observed with the cells irradiated by 3 Gy.
2.The mitochondrial membrane potentials increased immediately after 3 Gy and 9 Gy irradiated by 5.0 +/-2.1% and 5.6 +/-3.0%, respectively. At 8 and 12 hr following 9 Gy irradiation, the potentials decreased by 13.1 +/-2.1% and 15.9 +/-1.3%, respectively, although no significant changes in the potentials were noted with 3 Gy irradiated cells.
These findings suggested that the human cancer cells showed a transient increased mitochondrial metabolisms following irradiation, and gradual decreases.
3.Relationship between the mitochondrial metabolisms and cell cycle following irradiation was studied using by caffeine, an inhibitor of G2 block induced by x-ray radiation. With caffeine, the cell cycle distribution altered, however, it did not affected on the mitochondrial membrane potentials, suggesting the changes in the mitochondrial metabolisms following irradiation was independent of cell cycle.
4.When mild temperature hyperthermia (41℃, 1 hr) was administered before or after irradiation, the peak of mitochondrial membrane potentials noted immediately after irradiation was observed 0.5-1 hr after irradiation.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Masaya Furuta: "Monitoring mitochondrial metabolisms in irradiated human cancer cells with 99mTc-MIBI"Cancer Letters. (印刷中). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masaya Furuta: "Monitoring mitochondrial metabolisms in irradiated human cancer cells with 99mTc-MIBI"Cancer Letters. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Masaya Furuta, et al.: "Monitoring mitochondirial metabolisms in irradiated human cancer cells with ^<99m>Tc-MIBI"Cancer Letters. in press. (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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