| Project/Area Number |
13671005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Tottori University |
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Principal Investigator |
YOSHIOKA Shin-ichi Tottori University, Faculty of Medicine Associate professor, 医学部, 助教授 (00191544)
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| Co-Investigator(Kenkyū-buntansha) |
IMOTO Toshiaki Tottori University, Faculty of Medicine Associate professor, 医学部, 助教授 (10109639)
YOSIOKA Shin-ichi Tottori University, Faculty of Medicine Associate professor (00191544)
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| Project Period (FY) |
2001 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2001: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | amygdaloid kindling / seizure susceptibility / epileptogenesis / monosodium glutamate / methotrexate / immature / re-kindling / rat |
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| Research Abstract |
We investigated whether the primary and secondary epileptogenesis acquired in developing periods persists into adulthood using amygdaloid kindling rats. When the initial kindling was established at age 40 and 70 days, the number of stimulations required to induce the first stage 5 seizure at the contralateral as well as ipsilateral sites was significantly fewer in kindled rats than in non-stimulated controls. When the initial kindling was established at age 14 days the number of stimulations required to induce the first stage 5 seizure at neither ipsilateral or contralateral site in kindled rats was not significantly different from that in non-stimulated controls. When the initial kindling was established at age 28 days, the facilitative effect was observed at the ipsilateral site, but not at the contralateral site. These results suggest that both the primary and secondary epileptogenesis acquired at age 14 days is diminished after maturation, and the persistence of seizure susceptibility acquired in the developing periods is weaker in the primary than secondary site.
To clarify the mechanisms of the persistence of seizure susceptibility, we (MTX) treatments in rats modify the development of amygdaloid kindling in immature period and the persistence of seizure susceptibility during development. Both MSG and MTX treatments decreased significantly the number of stimulations required to reach the first stage 5 seizure in pups at age 14 days. When the rats were rekindled at age 70 days, the number of stimulations required to reinduce the first stage 5 seizure was significantly lower in rats treated with both MSG and MTX than that in control rats. These results suggest that the susceptibility to kindling-induced seizure in immature rats is enhanced with both MSG and MTX treatments, and that the enhanced seizure susceptibility acquired in immature period persists into maturity.
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