• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Expression of Ikaros isoforms in adult and childhood pre-B acute lymphoblastic leukemias.

Research Project

Project/Area Number 13671060
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionMie University

Principal Investigator

NISHII Kazuhiro  Mie University, Faculty of Medicine, Research Associate, 医学部附属病院, 助手 (50332713)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2001: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsacute leukemia / transcription factor / Ikaros / acute lymphoblastic leukemia / adult / childhood / molecular biology / cancer / 白血病 / 発癌機構 / リンパ性白血病 / 分子生分学
Research Abstract

Ikaros, a zinc finger transcription factor, is essential for lymphoid development. We examined the expression of Ikaros isoforms in 11 leukemic cell lines and adult acute lymphoblastic leukemia cells from 36 patients with B-precursor acute lymphoblastic leukemia (pre-B ALL) and nine with T-precursor acute lymphoblastic leukemia (pre-T ALL), using RT-PCR analysis. In one pre-B ALL cell line, INC cells, and primary leukemic cells from 16 patients with pre-B ALL, we found the predominant expression of a non-DNA-binding Ikaros isoform, Ik-6. However, Ik-6 was not detected in pre-T ALL cells. All of pre-B ALL cells expressing Ik-6 were CD10^+, whereas CD10^- pre-B ALL cells did not express Ik-6. The expression of Ik-6 was not related to karyotype abnormalities such as t(9 ; 22) and t(4 ; 11). Proteins from the cells that expressed Ik-6 alone failed to bind to the Ikaros protein-specific binding sequence in DNA. Ikaros proteins lacking the DNA binding sequences were detected in the cytoplasm … More but not in the nucleus of the cells. When INC and primary pre-B ALL cells that express Ik-6 alone were irradiated and cultured in the absence of serum, these cells produced functional Ikaros isoforms, lk-1 and Ik-2. Purified CD19^+ CD10^- and CD19^+ CD10^+ cells from normal human bone marrow did not express Ik-6. The predominant expression of Ik-6, which is the result of post-transcription dysregulation, is characteristic of adult pre-B ALL, especially CD10^+ pre-B ALL. We also determined if Ik6 was expressed in samples from 24 Japanese adults with AML, using RT-PCR ; 7 cases of M1, 5 of M2, 2 of M3, 5 with cessation of M4, and 5 with of M5. All AML samples expressed Ik1 and/or Ik2 but not Ik6. These findings indicate that Ik-6 is expressed in childhood AML but not in adult AML. Several reports demonstrated that Ik6 is frequently expressed in pre-B ALL of infants, young children, and adults, and in pre-T ALL of childhood. We reported that Ik-6 was detected in only adult pre-B ALL but not in adult pre-T ALL and AML.
Although the role of non-functional Ikaros isoforms in leukemogenic mechanisms is not fully understood, if should be kept in mine that Ik6 is expressed in a different manner among adult, childhood, and infant acute leukemias. Less

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Nishii K, Usui E, Katayama N, (+12).: "Characteristics of t(8;21) acute myeloid leukemia (AML) with additional chromosomal abnormality : concomitant trisomy 4 may constitute a distinctive subtype of t(8;21) AML"Leukemia. 17. 731-737 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakatsuka S-I, Takakuwa T, Tomita Y, Nishii K, (+5, 7^<th>).: "Hypermethylation of death-associated protein (DAP) kinase CpG island is frequent not only in B-cell but also in T-cell and natural killer (NK)/T-cell malignancies"Cancer Science. 94. 87-91 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Katayama N, Shiku H.: "Adult acute myeloid leukemia cells do not express non-functional Ikaros isoforms"Blood. 100. 3436-3437 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Katayama N, Miwa H, (+7).: "Non-DNA-binding Ikaros isoform expressed in adult B-precursor acute lymphoblastic leukemia"Leukemia. 16. 1285-1292 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masuya M, Nakatayama N, Nishii K, (+10, 10^<th>).: "Two independent clones in myelodysplastic syndrome following treatment of acute myeloid leukemia"International Journal of Hematology. 75. 182-186 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawakami K, Miyanishi S, Nishii K, (+4).: "A case of acute myeloid leukemia with t(7;11)(p15;p15) mimicking myeloid crisis of chronic myelogenous leukemia"International Journal of Hematology. 76. 80-83 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Mitani M, (+9).: "Successful treatment of invasive olumonary aspergillosis with G-CSF and M-CSF during long-term bone marrow suppression in hypoplastic leukemia"Rinshou Ketsueki. 43. 189-193 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Katayama N, Maeda H, (+6).: "Successful treatment of low dose splenic irradiation for massive splenomegaly in elderly Hairy Cell Leukemia patient"European Journal of Haematology. 67. 255-257 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Katayama N, Chen F, (+7).: "Aggressive neoplastic plasma cell growth with MLL gene rearrangement after high-dose therapy with autologous stem cell support for multiple myeloma"Bone Marrow Transplantation. 27. 555-558 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K., Usui E, Sakakura M, (+9).: "Additional translocation t(11;17)(q23;q21) in a patient with Philadelphia-positive mixed lineage antigen-expressing leukemia"Cancer Genetics and Cytogenetics. 126. 8-12 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakase K, KITA K, Nishii K, (+7, 4^<th>).: "Expression pattern of hybrid phenotype in adult acute lymphoblastic leukemia"Cancer Detection and Prevention. 25. 394-405 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Usui E, Katayama N, et al.: "Characteristics of t(8 ; 21) acute myeloid leukemia (AML) with additional chromosomal abnormality : Concomitant trisomy 4 may constitute a distictive subtype of t(8 ; 21) AML"Leukemia. 17. 731-737 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakatsuka S-I, Takakuwa T, Tomita Y, et al.: "Hypermethylation of death-associated protein (DAP) kinase CpG island is frequent not only in B-cell but also in T-cell and natural killer (NK)/T-cell malignancies"Cancer Science. 94. 87-91 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Katayama N, Shiku H.: "Adult acute myeloid leukemia cells do not express non-functional Ikaros isoforms"Blood. 100. 3436-3437 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Katayama N, Miwa H, et al.: "Non-DNA-binding Ikaros isoform expressed in adult B-precursor acute lymphoblastic leukemia"Leukemia. 16. 1285-1292 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Masuya M, Katayama N, Nishii K, et al.: "Two independent clones in myelodysplastic syndrome following treatment of acute myeloid leukemia"International Journal of Hematology. 75. 182-186 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kawakami K, Miyanishi S, Nishii K, et al.: "A Case of acute myeloid leukemia with t(7 ; 11)(p15 ; p15) mimicking myeloid crisis of chronic myelogenous leukemia"International Journal of Hematology. 76. 80-83 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Mitani M, et al.: "Successful treatment of invasive pulmonary aspergillosis with G-CSF and M-CSF during long-term bone marrow suppression in hypoplastic leukemia"Rinshou Ketsueki. 43. 189-193 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Katayama N, Maeda H, et al.: "Successful treatment of low dose splenic irradiation for massive splenomegaly in elderly Hairy Cell Leukemia patient"European Journal of Haematology. 67. 255-257 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Katayam N, Chen F, et al.: "Aggressive neoplastic plasma cell growth with MLL gene rearrangement after high-dose therapy with autologous stem cell support for multiple myeloma"Bone Marrow Transplantation. 27. 555-558 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Usui E, Sakakura M, et al.: "Additional translocation t(11 ; 17)(q23 ; p21) in a patient with Philadelphia-positive mixed lineage antigen-expressing leukemia"Cancer Genetics and Cytogenetics. 126. 8-12 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nakase K, Kita K, Nishii K, et al.: "Expression pattern of hybrid phenotype in adult acute lymphoblastic leukemia"Cancer Detection and Prevention. 25. 394-405 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nishii K, Usui E, Katayama N, (+12): "Characteristics of t(8;21) acute myeloid leukemia (AML) with additional chromosomal abnormality : Concomitant trisomy 4 may constitute a distinctive subtype of t(8;21) AML"Leukemia. (In press).

    • Related Report
      2002 Annual Research Report
  • [Publications] Nakatsuka S-I, Takakuwa T, Tomita Y, Nishii K, (+5, 7^<th>): "Hypermethylation of death-associated protein (DAP) kinase CpG island is frequent not only in B-cell but also in T-cell and natural killer (NK)/T-cell malignancies"Cancer Sci. 94. 87-91 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishii K, Katayama N, Shiku H.: "Adult acute myeloid leukemia cells do not express nonfunctional Ikaros isoforms"Blood. 100. 3436-3437 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishii K, Katayama N, Miwa H, (+7): "Non-DNA-binding Ikaros isoform expressed in adult B-precursor acute lymphoblastic leukemia"Leukemia. 16. 1285-1292 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishii K, Katayama N, Maeda H, (+6): "Successful treatment of low dose splenic irradiation for massive splenomegaly in elderly Hairy cell Leukemia patient"Eur J Haematol. 67. 255-257 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishii K, Usui E, Sakakura M, (+9): "Additional translocation t(11;17)(q23;q21) in a patient with Philadelphia-positive mixed lineage antigen-expressing leukemia"Cancer Genet Cytogenet. 126. 8-12 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Nishii K, et al.: "Non-DNA-binding Ikaros isoform expressed in adult B-precursor acute lymphoblastic leukemia"Leukemia. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishii K, et al.: "Successful treatment of low dose splenic irrodiation for massive splenomegaly in elderly Hairy Cell Leukemia patient"Eur J Haematol. 67. 255-257 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakase K, Kita K, Miwa H, Nishii K, et al.: "Expression pattern of hybrid phenotype in adult acute lymphoblastic leukemia"Cancer Detect Prev.. 25. 394-405 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishii K, et al.: "Aggressive neoplastic plasma cell growth with MLL gene rearrangement after high-dose therapy with autologaus stem cell suppovt for multiple myelona"Bone Marrow Transplant. 27. 555-558 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishii K, et al.: "Additional trarslocation +(11:17)(q23:q21)in a patient with Philadelphia-positive mixed lineage antigen-expressing leukemia"Cancer Genet Cytogenet. 126. 8-12 (2001)

    • Related Report
      2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi