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Study of the pathogenesis of Alport's syndrome and therapeutic approach to inhibit the progression of the clinical course.

Research Project

Project/Area Number 13671112
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionMie University

Principal Investigator

NOMURA Shinsuke  Mie University, School of Medicine, Associate Professor, 医学部附属病院, 助教授 (20198625)

Co-Investigator(Kenkyū-buntansha) NAITO Ichiro  Shigei Medical Institute, Department of Ultrastructured Pathology, Chief Director, 超微病理部門, 部長(研究職)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsAlport's syndrome / type IV collagen / glomerular basement membrane / Cyclosporine A / 遺伝子性糸球体腎炎 / IV型コラーゲンα / 遺伝性糸球体腎炎 / 進行性腎障害
Research Abstract

1)Early GBM lesion in Alport-mice
Observation of lesions in developping glomeruli using COL4A3-/-mouse as a human Alport's syndrome (AS) model has revealed that the most characteristic change in such period is the GBM outpockets observed through an electron microscope. Even during the period before the lamellation and splitting in the GBM is evident, the outpockets were observed in the mice more frequently and widely than those from wild type mice. It had been thought that outpockets are formed in the process of expansion of the basement membranes of developing glomeruli. However, the relationship between formation of outpockets and the changes such as lamellation and splitting appearing subsequently in the course of development should be studied.
2) Therapeutic approach for inhibiting the progression of Alport syndrome
Although several researchers have reported on human subjects who responded favorably to cyclosporine A (CyA), the reason why this immunosuppressant is effective in AS remains unclear. We administered CyA (3 mg/kg) to COL4A3-/-mice for only two weeks subcutaneouslly to evaluate its therapeutic effect and study the action mechanism. In the early treatment group in which CyA was started from 2 weeks of age. glomerulosclerosis and interstitial fibrosis at 8 weeks of age were remarkably inhibited. Immunohistologically. TGF-beta expression in glomerular mesangium was inhibited. In the late treatment group in which started 4weeks of age, the results were. These findings support the empirical, reports that CyA is effective for treatment of human AS. Although CyA is a costful and can bring significant adverse reactions, it should be considered as one of the therapeutic drugs.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Naito I., Ninomiya Y., Nomura S.: "Immunohistochemical diagnosis of Alport's syndrome in paraffin-embedded renal sections"Med Electron Microsc. 36・1. 1-7 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harvey SJ, Naito I, et al.: "Transfer of α5(IV) Collagen Chain Gene to Smooth Muscle Restores in Vivo Expression of the α6(IV) Chain in a Canine Model of Alport Syndrome"Am J Pathol. 162・3. 873-885 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harvey SJ, Naito, et al.: "The Inner Ear of Dogs with Alport nephritis provides clues to the pathogenesis of hearing loss in X-Linked Alport syndrome"Am J Pathol. 159・3. 1097-1104 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Borza DB, Naito I et al.: "The NC1 domain of collagen IV encodes a novel network composed of the α1, α2, α5, and α6 chains in smooth muscle basement membranes"J Biol Chem. 276・30. 28532-28540 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Naito I, Nomura S, et al.: "Immunohistochemical diagnosis of Alport's syndrome in peraffin embedded renal sections"Med Electron Microsc. 36(1). 1-7 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harvey SJ, Naito I, et al.: "Transfer of α5(IV) collagen chain gene to smooth muscle restores in vivo expression of α6(IV) collagen chain in a canine model of Alport syndrome"Am J Pathol. 162(3). 873-885 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Harvey SJ, Naito I, et al.: "The inner ear of dogs with X-linked nephritis provides clues to the pathogenesis of hearing loss in X-linked Alport syndrome"Am J.Pathol. 159(3). 1097-1104 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Borza DB, Naito I, et al.: "The NCl domain of collagen IV encodes a novel network composed of the α1, α2, α5 and α6 chains in smooth muscle basement membrane"J Biol Chem. 276(30). 28532-28540 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Naito I, Ninomiya Y, Nomura S: "Immunohistochemical diagnosis of Alport's syndrome in paraffin-embedded renal sections : antigen retrieval with autoclave heating"Med Electron Microsc. 36・1. 1-7 (2003)

    • Related Report
      2002 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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