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Molecular mechanism of spontaneous remission from renal injury

Research Project

Project/Area Number 13671118
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Kidney internal medicine
Research InstitutionNagasaki University

Principal Investigator

MIYAZAKI Masanobu  Nagasaki University, Hospital Medicine and Dentistry, Assistant Professor, 医学部・歯学部附属病院, 講師 (10246100)

Co-Investigator(Kenkyū-buntansha) KONHO Shigeru  Nagasaki University, Postgraduate School of Medicine, Professor, 大学院・医歯薬学総合研究科, 教授 (80136647)
Project Period (FY) 2001 – 2003
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2003: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2002: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsangiotensin II / aldosterone / angiogenesis / glomerulonephritis / VEGF / CYP11B2 / HSP47 / Ets-1 / 腎炎 / 再生 / マトリックスメタロプロテナーゼ / 骨髄細胞
Research Abstract

In the present study, we examined the role of angiotensin II in the process of spontaneous remission of glomerular injury in experimental mesangial proliferative glomerulonephritis and tested the expression of mRNA for CYP11B, the key enzyme of aldosterone synthesis in human renal biopsy specimens using in situ hybridization. We induced reversal mesangial proliferative glomerulonephritis by Habu-snake venom injection. Compared with wild type mice, the delay of remission from glomerular injury, including expansion of mesangial area and reduced number of glomerular capillary wall, was observed in mice without angiotensin II receptor type 1. Treatment of VEGF (vascular endothelial cell growth factor) promoted restoration of glomerularr injury in the model. In the second study, CYP11B2 mRNA was expressed in glomerular cells, such as mesangial cells, glomerular epithelial and endothelial cells, and infiltrating cells and atrophic tubular cells in the interstitium. Moreover, these expressions of CYP 11 B2 mRNA were significantly correlated with the degree of their tissue injury. Our results suggested that angiotensin II is important in the reconstitution of glomerular capillary and that aldosterone produced in human renal tissue was involved in renal tissue damage.

Report

(4 results)
  • 2003 Annual Research Report   Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Miyazaki M, Nishio T, Abe K, Furusu A, Koji T, Kohno S: "Regulation of renal extracellular matrix metabolism"Contrib Nephrol. 139. 141-155 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ashizawa M.Miyazaki M, Abe K, et al.: "Detection of nuclear factor-kB in IgA nephropathy using Southwestern histocheroistry."Am J Kid Dis. 42(1). 76-86 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishino T, Miyazkai M, Abe K, et al.: "Antisense oligonucleotides against collagen-binding stress protein HSP 47 suppress peritoneal fibrosis in rats"Kidney Int. 64(3). 887-896 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mishima Y, Miyazkai M, Abe K, et al.: "Enhanced expression of heat shock protein 47 in rat model of peritoneal fibrosis."Perit Dial Int. 23(1). 14-22 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Horita Y.Hayashida K, Fukuchi, K, et al.: "Acetazolaraide assisted Tc-99m MAG3 renography to assess renal blood flow reserve."Ann Nucl Med. 17(2). 139-144 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyazaki M, Nishio T, Abe K, Furusu A, Koji T, Kohno. S: "Regulation of renal extracellular matrix metabolism"Contrib Nephrol. 139. 141-155 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ashizawa M, Miyazaki M, Abe K, et al.: "Detection of nuclear factor-kB in IgA nephropathy using Southwestern histochemistry."Am J Kid Dis. 42(1). 76-86 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishino T, Miyazkai M, Abe K, et al.: "Antisense oligonucleotides against collagen-binding stress protein HSP47 suppress peritoneal fibrosis in rats"Kidney Int. 64(3). 887-896 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Mishima Y, Miyazkai M, Abe K, et al.: "Enhanced expression of heat shock protein 47 in rat model of peritoneal fibrosis."Pent Dial Int. 23(1). 14-22 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Horita Y, Hayashida K, Fukuchi, K, et al.: "Acetazolamide assisted Tc-99m MAG3 renography to assess renal blood flow reserve."Ann Nucl Med. 17(2). 139-144 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Miyazaki M, Nishio T, Abe K, Furusu A, Koji T, Kohno S: "Regulation of renal extracellular matrix metabolism"Contrib Nephrol. 139. 141-155 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ashizawa M, Miyazaki M, Abe K, et al.: "Detection of nuclear factor-kB in IgA nephropathy using Southwestern histochemistry"Am J Kid Dis. 42(1). 76-86 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nishino T, Miyazkai M, Abe K, et al.: "Antisense oligonucleotides against collagen-binding stress protein HSP47 suppress peritoneal fibrosis in rats"Kidney Int. 64(3). 887-896 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Mishima Y, Miyazkai M, Abe K, et al.: "Enhanced expression of heat shock protein 47 in rat model of peritoneal fibrosis"Perit Dial Int. 23(1). 14-22 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Horita Y, Hayashida K, Fukuchi, K, et al.: "Acetazolamide assisted Tc-99m MAG3 renography to assess renal blood flow reserve"Ann Nucl Med. 17(2). 139-144 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Miyazaki M: "Regulation of renal extracellular matrix metabolism"Contrib Nephrol. (印刷中).

    • Related Report
      2002 Annual Research Report
  • [Publications] Ota T: "Expression of monocyte chemoattractant protein-1 in proximal tubular epithelial cells in a rat model of progressive kidney failure"J Lab Clin Med. 140. 43-51 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Abe K: "Enhanced expression of complement C5a receptor mRNA in human diseased kidney assessed by in situ hybridization"Kidney Int. 60(1). 137-146 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Abe K: "Intraglomerular complement C3 synthesis and its activation in IgA nephropathy"Nephron. 87(3). 231-239 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Abe K, Miyazaki M, Koji K et al.: "Intraglomerular complement C3 synthesis and its activation in IgA nephropathy"Nephron. 87(3). 231-239 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Abe K, Miyazaki M, Koji T, et al.: "Enhanced expression of complement C5a receptor mRNA in human diseased kidney assessed by in situ hybridization"Kidney International. 60(1). 137-146 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nishino T, Miyazaki M, Kohno S: "Regulation of renal extracellular matrix metabolism"Renal Fibrosis edited by Takashi Taguchi, New Delhi, Research Signport. (印刷中). (2002)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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