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Mechanism of action of ghrelin

Research Project

Project/Area Number 13671148
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionThe University of Tokyo

Principal Investigator

TAKANO Koji  The University of Tokyo, Faculty of Medicine, research associate, 医学部附属病院, 助手 (20236243)

Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 2002: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2001: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsGhrelin / Grow hormone / Channel / alectrophysiology / secretion / Ghrelin / channel / electro physiology / secretion / ghrelin(グレリン) / 成長ホルモン / イオンチャネル / 電気生理学 / 非選択性腸イオン電流 / 細胞内カルシウム濃度 / ホルモン分泌
Research Abstract

The mechanism of ghrelin-induced excitation of human GH-secreting cells was investigated using perforated- whole cell clamp technique and measurement of intracellular Ca^<2+> concentration [Ca^<2+>]I. Ghrelin depolarized GH-secreting human pituitary adenoma cells and increased action potential frequency. The depolarization was induced by a Na^+ influx through nonselective cation channels. The signal transduction of ghrelin-induced activation of the nonselective cation current involved protein kinase C. Ghrelin increased intracellular Ca^<2+> concentration of these cells depending mainly on the Ca^<2+> influx through the voltage-gated Ca^<2+> channels. This Ghrelin-induced increase in [Ca^<2+>]I was abolished in Na^+-free extracellular solution but was not abolished by tetrodotoxin, indicating that the ghrelin-induced cation current and depolarization are important in ghrelin-induced [Ca^<2+>]I increase. Ghrelin increased GH secretion dependency on the voltage-gated Ca^<2+> influx. In non-adenoma human GH-secreting cells, ghrelin-induced activation of a nonselective cation current and depolarization was also observed. These data indicate that ghrelin stimulates GH secretion by excitating these cells and increasing Ca^<2+> influx through the voltage-gated Ca^<2+> channels in human GH-secreting cells.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Sandrini F et al.: "PRKAR1A, one of the Carney Complex genes"J. Med. Genet. 39. e78-e88 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura Y et al.: "A Kindred of familial acromegaly without evidence for linkage to MEN-1 locus"Endocr. J. 49. 425-431 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K Takano.K et al.: "Ionic mechanism of action of ghrelin"Proc. of Endocine. Soc. 524 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K Takano K et al.: "Mechanism of action of ghrelin"Proc. 6th Pituitary Congress. 87 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano, K., J. Yasufuku-Takano, A, Teramoto, and T. Fujita.: "Ionic mechanism of action of ghrelin on human GH-secreting cells"Proc. Endoc. Soc.. 524 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takano, K., J. Yasufuku-Tkano, A. Teramoto, and T. Fujita.: "Mechanism of action of ghrelin on human somatotroph adenoma cells"Proc. 6^<th> Pituitary Congress.. 87. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sandrini F, Kirschner LS, Bei-T, Farmakidis C, Yasufuku-Takano J, Takano K: "Prezant TR, Marx SJ, Farrell WE, Clayton RN, Groussin L, Bertherat J, Stratakis CA. PRKAR1A, one of the Carney complex genes, and its locus (17q22-24) are rarely altered in pituitary tumours outside the Carney complex"J Med Genet.. 39(12). e78 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Tamura Y, Ishibashi S, Gotoda T, Yasufuku-Takano J, Takano K, Ueki K, Yamashita S, Iizuka Y, Yahagi N, Shionoiri F, Okazaki H, Ohashi K, Osuga J, Harada K, Shimano H, Fujita T, Yamada N, Kimura S.: "A kindred of familial acromegaly without evidence for linkage to MEN-1 locus"Endocr J.. 49(4). 425-431 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Sandrini, F et al.: "PKAR1A, one of the Carney complex genes"J.Med Genet.. 39(12). e75-e85 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Tamura, Y et al.: "A Kindred of familial acromegaly without evidence of ・・・"Endocrine J. 49(4). 425-431 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takano, K et al.: "Iionic mechanism of action shrelin on human GH-secretins cells"Prac.Endocrine Society. 524 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Takano, K. et al.: "Mechanism of action of ghrelin on human somatotroph adenoma cells"Proc.6^<th> Pituitary Congress. 87 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Koji Takano et al.: "Ghrelin excites human GH secreting cells"Proceedings of the 83rd Endocine Society's Annual Meeting. 234 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Koji Takano et al.: "Ionic Mechanism of Ghrelin-Induced GH secretion"Proceedings of the 7^<th> International Pitaitary Congress. 60 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Toshio Ichikawa et al.: "Estrogenic Imparities in Plastic Labwares"Nature Biotechnology. 19. 812 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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