• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Gene therapy for induction of immunological tolerance in organ allografts

Research Project

Project/Area Number 13671253
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionSHOWA University

Principal Investigator

KATO Hirohisa  SHOWA University, Medicine, Assistant Professor, 医学部, 助手 (10286784)

Co-Investigator(Kenkyū-buntansha) KUSANO MITSUO  SHOWA University, Medicine, Chief Professor, 医学部, 教授 (70091569)
MURAKAMI MASAHIKO  SHOWA University, Medicine, Associate Professor, 医学部, 助教授 (70255727)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2002: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsORGAN TRANSPLANTATION / GENE TRANSFER / TOLERANCE
Research Abstract

(Background) Gene transfer approach has the potential to introduce immunosuppressive molecules directly into the allograft, which in turn should limit systemic side effects of conventional immunosuppressive therapy to recipients. (Aim) To analyze the efficacy and putative mechanisms of adenoviral-mediated IL4 gene transfer in rat kidney transplant model. (Material & Method) Renal allografts (BN rat) were perfused in-situ with Adex1CAmIL4 or AxCALacZ (RIKEN DNA Bank, respectively) incubated at 4C for 1.5, 24, 48, 72h (ex-vivo), or/and transplanted to LEW recipients. Adenovirus vector was perfused with 4C saline or UW solution, and renal grafts were preserved in 4C saline (1.5h) or UW solution (24-72h). (Results) 1. β-gal was detected after 24h preservation, but not 1.5h in ex-vivo model. After transplantation, there was mo staining of β-gal in renal allograft at day2/day7. 2. In ex-vivo preservation, IL4 was expressed in all of renal grafts (1.5h-72h) with Adex1CAmIL4 perfusion, even though it was not detected in control group perfused with cold saline in any preserved term. 3. Histological damage of renal allografts maintained for 24h after Adex1CAmIL4 gene transfer were diminished as compared with 1.5h preserved grafts at day7 after transplantation. (Conclusion) 24h preservation was prefer to induce immunosuppressive effects of Adenovirus-mediated IL4 gene therapy as compared. with 1.5h maintaining. It seems that appropriate expression of IL4 might be important at the early phase (day0-2) after transplantation. Further study will be necessary to inquire "optimal way" of gene transfer in transplant setting.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report

URL: 

Published: 2001-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi