Biochyperhemical evaluation of isolated porcine liver system perfused with oxygenated blood by ECMO
| Project/Area Number |
13671262
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kanazawa Medical University |
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Principal Investigator |
IKAWA Hiromiti Kanazawa Medical University, Professor, 医学部, 教授 (20124935)
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| Co-Investigator(Kenkyū-buntansha) |
MASUYAMA Hiroaki Kanazawa Medical University, Assistant, 医学部, 助手 (90255480)
OKAMOTO Sinya Kanazawa Medical University, Assistant Professor, 医学部, 講師 (00329377)
岡島 英明 金沢医科大学, 医学部, 助手 (20308604)
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| Project Period (FY) |
2001 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2003: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Liver transplantation / perfusion preservation / hyperglyicemia |
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| Research Abstract |
Aim : The optimal condition of liver perfusion was investigated in order to prolong the preservation time of liver graft and also to avoid primary non-function or re-perfusion injury.
Materials and Methods : Twenty one porcine livers were purfused with oxygenated blood by ECMO, using venous-venous bypass via portal vein to infrahepatic IVC. After supra IVC and hepatic artery were ligated, thliver was harvested and kept on perfusing in 37℃ lactate Ringer solution.
Results : Since some amount of bile juice was excreted throughout the experiment, biological activity of the liver grafts seem to be maintained essentially by meaning of perfusion with oxygenated blood for more than 12 hours. However, it was made clear that the oxygenated perfusion via portal vein be responsible for severe metabolic and biochemical problems. They were hyponatremia, metabolic asidosis and hyperglycemia which were resistant to insulin administration.
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Report
(4 results)
Research Products
(6 results)
