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Efficacy of Double Filtration Plasmapheretic Cross Circulation with a High Permeability Membrane using Whole Liver on Porcine Fulminant Hepatic Failure Model

Research Project

Project/Area Number 13671272
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionHirosaki University

Principal Investigator

TOTSUKA Eishi  Shool of Medicine, Hirosaki University Instructor, 医学部, 助手 (70281920)

Co-Investigator(Kenkyū-buntansha) NARUMI Shunji  University Hospital, Hirosaki University Instructor, 医学部附属病院, 助手 (90250612)
HAKAMADA Kenichi  University Hospital, Hirosaki University Assistant Professor, 医学部附属病院, 講師 (30271802)
SASAKI Matsuo  Shool of Medicine, Hirosaki University Professor, 医学部, 教授 (10005077)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2002: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsBioartificial liver / Plasmapheresis / Semipermeable membrane / Fulminant hepatic failure / 摘出肝異種交叉灌流 / ブタ / 交差灌流
Research Abstract

In the use of bioartificial liver devices, preparation of sufficient liver cell mass to provide adequate metabolic support, reduction of xenogeneic immune reaction, and avoidance of viral transmission are required. We developed a plasmapheresis using semipermeable membrane combined with whole liver perfusion (PMWLP) system, which offered a large number of functional liver cells, an immunoprotection, and a barrier to the viral transmission. The effect of the PMWLP system in porcine FHF model was investigated. Porcine FHF model was established by the intraportal administration of alpha-amanitin and lipopolysaccharide. Nine hours after the drugs injection, the FHF pigs were connected to the PMWLP system for 6 hours (n=5). In control pigs (n=10), the circuit was not connected to the porcine whole liver. Animal survival was significantly longer in the PMWLP treated pigs. Although the control pigs developed hypotension progressively, the PMWLP treated pigs demonstrated a cardiovascular stabilization. The PMWLP treatment significantly suppressed the elevation of liver enzymes, total bile acid, ammonia, and metionine. The degrees of hepatocyte necrosis and neutrophil infiltration were remarkably lessened in the PMWLP treated pigs. The PMWLP system prolonged animal survival, prevented cardiovascular instability, attenuated liver injury, enhanced bile acid metabolism, and decreased the hepatic encephalopathy related agents.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Totsuka E, Itoh S, Suzuki K, et al.: "Usefulness of redox to lerance test in evaluating fatty liver"Hepato-Gastroenterol. 48. 184-187 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nara M, Hakamada K, Totsuka E, et al.: "Efficacy of double filtration plasmapheretic cross-circulation using a high permeability membrane between totally hepatectomized dogs and donor pigs"Transplantation. 72. 1736-1742 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takiguchi M, Totsuka E, Umehara M, et al.: "Attenuation of canine hepatic warm ischemia-reperfusion injury associated with neutrophil infiltration by cytokine suppressive anti-inflammatory agent, ER 167653"Hirosaki Med J. 53. 26-32 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nara M, Hakamada K, Totsuka E, et al.: "Xenogeneic bioartificial liver support by double filtration plasmapheretic cross circulation using a high performance semipermeable membrane"Hirosaki Med J. 53. 104-110 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Totsuka E, Fung JJ, Lee MC, et al.: "Influence of cold ischemia time and graft transoprt distance on postoperative outcome in human liver transplantation"Surg Today. 32. 792-799 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takiguchi M, Totsuka E, Umehara M, et al.: "Attenuation of microcirculatory disturbance after liver ischemia by newly synthesized inflammatory cytokine suppressor, FR 167653"Hepato-gastroenterol. 50. 789-793 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 十束英志, Fung JJ, 梅原実, 他: "心停止及び心停止後蘇生ドナーからの肝移植の経験"今日の移植. 15. 100-104 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nozaki T, Totsuka E, Takiguchi M, et al.: "Attenuation of canine hepatic warm ischemia/reperfusion injury by nitric oxide donor, FK409"Hirosaki Med J. (印刷中). (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Totsuka E, Itoh S, Suzuki K, et al.: "Usefulness of redox tolerance test in evaluating fatty liver"Hepato-Gastroenterol. 48. 184-187 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nara M, Hakamada K, Totsuka E, et al.: "Efficacy of double filtration plasmapheretic cross-circulation using a high permeability membrane between totally hepatectomized dogs and donor pigs"Transplantation. 72. 1736-1742 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takiguchi M, Totsuka E, Umehara M, et al.: "Attenuation of canine hepatic warm ischemia-reperfusion injury associated with neutrophil infiltration by cytokine suppressive anti-inflammatory agent, ER167653"Hirosaki Med J. 53. 26-32 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nara M, Hakamada K, Totsuka E, et al.: "Xenogeneic bioartificial liver support by double filtration plasmapheretic cross circulation using a high performance semipermeable membrane"Hirosaki Med J. 53. 104-110 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Totsuka E, Fung JJ, Lee MC, et al.: "Influence of cold ischemia time and graft transoprt distance on postoperative outcome in human liver transplantation"Surg Today. 32. 792-799 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takiguchi M, Totsuka E, Umehara M, et al.: "Attenuation of microcirculatory disturbance after liver ischemia by newly synthesized inflammatory cytokine suppressor, FR167653"Hepato-gastroenterol. 50. 789-793 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nozaki T, Totsuka E, Takiguchi M, et al.: "Attenuation of canine hepatic warm ischemia/reperfusion injury by nitric oxide donor, FK409"Hirosaki Med J. In Press. (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Nara M.: "Efficacy of double filtration plasmapheretic cross-circulation using a high permeability membrane between totally hepatectomized dogs and donor pigs"Transplantation. 72(11). 1736-1742 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2002-04-01   Modified: 2016-04-21  

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