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Cytochrome P450 and Cyclooxygenease-2 expression in liver tissue surrounding hepatocellur carcinoma : Evaluation of environmental state of multicentric hepatocarcinogenesis

Research Project

Project/Area Number 13671283
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionCHIBA UNIVERSITY

Principal Investigator

ITO Hiroshi  Chiba University, Graduate School of Medicine, Associate Professor, 大学院・医学研究院, 助教授 (00232463)

Co-Investigator(Kenkyū-buntansha) YOSHIDOME Hiroyuki  Chiba University, University Hospital, Assistant, 医学部附属病院, 助手 (10312935)
ISHIKURA Hiroshi  Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (70222982)
MIYAZAKI Masaru  Chiba University, Graduate School of Medicine, Professor, 大学院・医学研究院, 教授 (70166156)
OHTSUKA Masayuki  Chiba University, University Hospital, Assistant, 医学部附属病院, 助手 (90334185)
Project Period (FY) 2001 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2002: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2001: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsHepatocellular Carcinoma / Carcinogenesis / Oxidative Stress / Cytochrome P450 / Cyclooxygenase-2 / Multicentric Occurrence
Research Abstract

BACKGROUND. Hepatocellular carcinoma (HCC) usually originates in chronic hepatitis or liver cirrhosis. Cyclooxygenase-2 (COX-2), a key enzyme in the conversion of arachidonic acid to prostaglandins, and cytochrome P450 (CYP), xenobiotics metabolizing enzymes, are said to be involved in carcinogenesis. In this study, we explored if COX and CYP expression in injured liver tissue was involved in hepatocarcinogenesis, especially multicentric occurrence of HCC.
METHODS. The expression of cyclooxygenase-2 (COX-2) and cytochrome P450 (CYP) in liver tissues from surgical specimens were immunohistochemically examined, and malondialdehyde (MDA) level in liver tissue was also determined.
RESULTS. COX-2 expression in liver tissue surrounding tumor was significantly higher in cases with HCC than in cases with normal liver, and the expression rate of COX-2 grew higher in proportion to degree of fibrosis. There was no difference between cases with HCC and those with normal liver as regards CYP isoform expression in liver tissue surrounding tumors. With all CYP isoforms, the co-expression rate of COX-2 and CYP tended to be higher in cases with HCC than in cases with normal liver, and the co-expression rate of COX-2 and CYP2E1 was significantly higher in the multicentric occurrence (MO) group than in the non-multicentric occurrence (non-MO) group. Moreover, the MDA volume was significantly higher in the MO group (0.633 ± 0.473 μg/mg protein) than in the non-MO group (0.303 ± 0.257, p<0.05).
CONCLUSIONS. Our data suggest that COX-2 and CYP may influence the hepatocarcinogenesis, especially in their multicentric occurrence.

Report

(3 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Shiobara Masayuki: "Enhanced polyadenosine diphosphate-ribosylation in cirrhotic liver and carcinoma tissue in patients with hepatocellular carcinoma"Journal of Gastroentelogy-Hepatology. 16. 338-344 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimizu Hiroaki: "Vascular endothelial growth factor secreted by replicating hepatocytes induce sinusoidal endothelial cell proliferation during regeneration after partial hepatectomy in rats"Journal of Hepatology. 34. 683-689 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kataoka Masaaki: "Effect of cold-ischemia time on C-X-C chemokine expression and neutrophil accumulation in the graft liver after orthotopic liver transplantation in rats"Transplantation. 73. 1730-1735 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shiobara M, Miyazaki M, Ito H, Togawa A, Nakajima N, Nomura F, Morinaga N, Noda M: "Enhanced polyadenosine diphosphate-ribosylation in cirrhotic liver and carcinoma tissue in patients with hepatocellular carcinoma"Journal of Gastroenterol. Hepatology. 16. 338-344 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shimizu H, Miyazaki M, Wakabayashi Y, Mitsuhashi N, Kato A, Ito H, Nakagawa K, Yoshidome H, Kataoka M, Nakajima N: "Vascular endothelial growth factor secreted by replicating hepatocytes induce sinusoidal endothelial cell proliferation during regeneration after partial hepatectomy in rats"Journal of Hepatology. 34. 683-689 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Kataoka M, Shimizu H, Mitsuhashi N, Ohtsuka M, Wakabayashi Y, Ito H, Kimura F, Nakagawa K, Yoshidome H, Shimizu Y, Miyazaki M: "Effect of cold ischemia time on C-X-C chemokine expression and neutrophil accumilation in the graft live after orthotopic liver transplantation in rats"Transplantation. 73. 1730-1735 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shiobara Masayuki: "Enhanced polyadenosine diphosphate-ribosylation in cirrhotic liver and carcinoma tissue in patients with hepatocellular carcinoma"Journal of Gastroentelogy-Hepatology. 16. 338-344 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shimizu Hiroaki: "Vascular endothelial growth factor secreted by replicating hepatocytes induce sinusoidal endothelial cell proliferation during regeneration after partial hepatectomy in rats"Journal of Hepatology. 34. 683-689 (2001)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kataoka Masaaki: "Effect of cold-ischemia time on C-X-C chemokine expresssion and neutrophil accumulation in the graft liver after orthotopic liver taransplantation in rats"Transplantation. 73. 1730-1735 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shiobara Masayuki: "Enhanced polyadenosine diphosphate-ribosylation in cirrhotic liver and carcinoma tissue in patients with hepatocellular carcinoma"Journal of Gastroentelogy-Hepatology. 16. 338-344 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shimizu Hiroaki: "Vascular endothelial growth factor secreted by replicating hepatocytes induce sinusoidal endothelial cell proliferation during regeneration after partial hepatectomy in rats"Journal of Hepatology. 34. 683-689 (2001)

    • Related Report
      2001 Annual Research Report

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Published: 2001-04-01   Modified: 2016-04-21  

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